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- PDB-8c10: Biochemical and structural characterisation of an alkaline family... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8c10 | ||||||
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Title | Biochemical and structural characterisation of an alkaline family GH5 cellulase from a shipworm symbiont | ||||||
![]() | GH5 Cellulase | ||||||
![]() | HYDROLASE / GH family 5 / shipworm / symbiont / enzymatic hydrolysis. | ||||||
Function / homology | : ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Leiros, I. / Vaaje-Kolstad, G. | ||||||
Funding support | 1items
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![]() | ![]() Title: Biochemical and structural characterisation of a family GH5 cellulase from endosymbiont of shipworm P. megotara. Authors: Junghare, M. / Manavalan, T. / Fredriksen, L. / Leiros, I. / Altermark, B. / Eijsink, V.G.H. / Vaaje-Kolstad, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 150.4 KB | Display | ![]() |
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PDB format | ![]() | 115.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.8 MB | Display | ![]() |
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Full document | ![]() | 2.8 MB | Display | |
Data in XML | ![]() | 17.8 KB | Display | |
Data in CIF | ![]() | 27.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 35278.766 Da / Num. of mol.: 1 / Mutation: A25M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 5 types, 357 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ChemComp-TRS / | #4: Chemical | #5: Chemical | ChemComp-K / | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.41 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5 25 % w/v PEG 3350 Temp details: room temperature |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 1, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91842 Å / Relative weight: 1 |
Reflection | Resolution: 1→45.04 Å / Num. obs: 143241 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.6 % / Biso Wilson estimate: 6.32 Å2 / Rmerge(I) obs: 0.077 / Net I/σ(I): 11.5 |
Reflection shell | Resolution: 1→1.02 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.609 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 5490 / % possible all: 77.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 10.485 Å2
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Refinement step | Cycle: 1 / Resolution: 1→53.83 Å
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Refine LS restraints |
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