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- PDB-8bvk: The crystal structure of O-glycoside cleaving beta-eliminase from... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8bvk | ||||||
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Title | The crystal structure of O-glycoside cleaving beta-eliminase from A. tumefaciens AtOGE | ||||||
![]() | Xylose isomerase | ||||||
![]() | HYDROLASE / O-glycoside cleaving beta-eliminase / A. tumefaciens / AtOGE | ||||||
Function / homology | Xylose isomerase-like, TIM barrel domain / Xylose isomerase-like TIM barrel / Xylose isomerase-like superfamily / isomerase activity / : / Xylose isomerase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kuhlmann, K. / Bitter, J. / Pfeiffer, M. / Nidetzky, B. / Pavkov-Keller, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Enzymatic beta-elimination in natural product O- and C-glycoside deglycosylation. Authors: Bitter, J. / Pfeiffer, M. / Borg, A.J.E. / Kuhlmann, K. / Pavkov-Keller, T. / Sanchez-Murcia, P.A. / Nidetzky, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127.2 KB | Display | ![]() |
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PDB format | ![]() | 77.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 20.3 KB | Display | |
Data in CIF | ![]() | 29.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27906.691 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.2 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion, sitting drop Details: 0.2 M ammonium chloridenitrate, containing 20% (w/v) PEG 3350; 10 mg/mL AtOGE1 in buffer C (10 mM HEPES buffer pH 7.0 containing 150 mM NaCl and 0.1 mM TCEP) and 0.04 mM MnCl2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 8, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 |
Reflection | Resolution: 1.995→46.84 Å / Num. obs: 55224 / % possible obs: 99.03 % / Redundancy: 12.5 % / Biso Wilson estimate: 28.81 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.0794 / Rpim(I) all: 0.0234 / Rrim(I) all: 0.0829 / Net I/σ(I): 20.3 |
Reflection shell | Resolution: 1.995→2.067 Å / Redundancy: 12 % / Rmerge(I) obs: 0.291 / Mean I/σ(I) obs: 6.24 / Num. unique obs: 2699 / CC1/2: 0.985 / CC star: 0.996 / Rpim(I) all: 0.0859 / Rrim(I) all: 0.3038 / % possible all: 91.99 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.22 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→46.84 Å
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Refine LS restraints |
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LS refinement shell |
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