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Open data
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Basic information
Entry | Database: PDB / ID: 8btj | ||||||
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Title | Murine cytomegalovirus protein M35 | ||||||
![]() | Protein M35 | ||||||
![]() | STRUCTURAL PROTEIN / CMV / M35 / virus protein / NFKB mediated transcription | ||||||
Function / homology | Herpesvirus phosphoprotein 85 (HHV6-7 U14/HCMV UL25) / Herpesvirus phosphoprotein 85 (HHV6-7 U14/HCMV UL25) / host cell nucleus / (R,R)-2,3-BUTANEDIOL / MALONATE ION / Protein M35![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schmelz, S. / Van den Heuvel, J. / Blankenfeldt, W. | ||||||
Funding support | 1items
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![]() | ![]() Title: The Cytomegalovirus M35 Protein Directly Binds to the Interferon-beta Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes. Authors: Schwanke, H. / Goncalves Magalhaes, V. / Schmelz, S. / Wyler, E. / Hennig, T. / Gunther, T. / Grundhoff, A. / Dolken, L. / Landthaler, M. / van Ham, M. / Jansch, L. / Bussow, K. / van den ...Authors: Schwanke, H. / Goncalves Magalhaes, V. / Schmelz, S. / Wyler, E. / Hennig, T. / Gunther, T. / Grundhoff, A. / Dolken, L. / Landthaler, M. / van Ham, M. / Jansch, L. / Bussow, K. / van den Heuvel, J. / Blankenfeldt, W. / Friedel, C.C. / Erhard, F. / Brinkmann, M.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 395.8 KB | Display | ![]() |
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PDB format | ![]() | 263.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50834.816 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | ChemComp-BU3 / ( #3: Chemical | ChemComp-MLI / | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.2 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 1.1M Na2 Malon, 0.1 M HEPES pH 7.0, 0.5 % (v/v) Jeffamine ED-2001 protein conc: 3.6mg/ml cryoprotectant: 12 (v/v) (2R,3R) -(-)-2,3-Butanediol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 12, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 1.94→66.52 Å / Num. obs: 50243 / % possible obs: 92 % / Redundancy: 10.6 % / Biso Wilson estimate: 29.79 Å2 / CC1/2: 0.999 / Rpim(I) all: 0.047 / Net I/σ(I): 11.6 |
Reflection shell | Resolution: 1.94→2.151 Å / Num. unique obs: 2513 / CC1/2: 0.581 / Rpim(I) all: 0.488 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.96 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.94→57.95 Å
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Refine LS restraints |
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LS refinement shell |
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