+Open data
-Basic information
Entry | Database: PDB / ID: 8brg | ||||||
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Title | Crystal structure of She4 | ||||||
Components | KLLA0E16699p | ||||||
Keywords | CHAPERONE / UNC-45 / UCS / myosin | ||||||
Function / homology | UNC-45/Cro1/She4, central domain / Myosin-binding striated muscle assembly central / chaperone-mediated protein folding / Hsp90 protein binding / Armadillo-like helical / Armadillo-type fold / cytoplasm / KLLA0E16699p Function and homology information | ||||||
Biological species | Kluyveromyces lactis (yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Gudino, R. / Arnese, R. / Meinhart, A. / Clausen, T. | ||||||
Funding support | Austria, 1items
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Citation | Journal: Nat Commun / Year: 2024 Title: UNC-45 assisted myosin folding depends on a conserved FX 3 HY motif implicated in Freeman Sheldon Syndrome. Authors: Vogel, A. / Arnese, R. / Gudino Carrillo, R.M. / Sehr, D. / Deszcz, L. / Bylicki, A. / Meinhart, A. / Clausen, T. #1: Journal: bioRxiv / Year: 2022 Title: UNC-45 assisted myosin folding depends on a conserved FX3HY motif implicated in Freeman Sheldon Syndrome Authors: Vogel, A. / Arnese, R. / Gudino, R. / Sehr, D. / Bylicki, A. / Meinhart, A. / Clausen, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8brg.cif.gz | 296.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8brg.ent.gz | 214.1 KB | Display | PDB format |
PDBx/mmJSON format | 8brg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8brg_validation.pdf.gz | 434.9 KB | Display | wwPDB validaton report |
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Full document | 8brg_full_validation.pdf.gz | 454.1 KB | Display | |
Data in XML | 8brg_validation.xml.gz | 25 KB | Display | |
Data in CIF | 8brg_validation.cif.gz | 33.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/br/8brg ftp://data.pdbj.org/pub/pdb/validation_reports/br/8brg | HTTPS FTP |
-Related structure data
Related structure data | 8brhC 5mzuS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 75856.875 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: She4 / Source: (gene. exp.) Kluyveromyces lactis (yeast) / Gene: KLLA0_E16699g / Production host: Escherichia coli (E. coli) / References: UniProt: Q6CMY7 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 50.07 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.1 M MOPS/Hepes pH 7.5, 10% PEG 8000,20% Ethylene glycol, 0.03 mM CaCl2 and 0.03 mM MgCl2 |
-Data collection
Diffraction | Mean temperature: 110 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, DESY / Beamline: P11 / Wavelength: 0.976261 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 6, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976261 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→19.83 Å / Num. obs: 29424 / % possible obs: 99.74 % / Redundancy: 19.7 % / Biso Wilson estimate: 58.65 Å2 / CC1/2: 1 / Net I/σ(I): 19.71 |
Reflection shell | Resolution: 2.4→2.486 Å / Num. unique obs: 2908 / CC1/2: 0.589 / % possible all: 99.97 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5MZU Resolution: 2.4→19.83 Å / SU ML: 0.3009 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 27.4881 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 81.29 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→19.83 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION / Auth asym-ID: A / Label asym-ID: A
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