PDB-8bcq: N-terminal domain of Plasmodium berghei glutamyl-tRNA synthetase ...

ID or keywords:

Entry

Database: PDB / ID: 8bcq

Title

N-terminal domain of Plasmodium berghei glutamyl-tRNA synthetase (native crystal structure)

Components

Glutamate--tRNA ligase

Keywords

RNA BINDING PROTEIN /

aminoacyl-tRNA synthetase /

tRNA /

protein complex /

Cell surface protein / multi-aminoacyl-tRNA synthetase complex (MARS-MSC) / GST-like domain / aminoacyl-tRNA synthetase-interacting multifunctional proteins (AIMP)

Function / homology

Function and homology information

glutamate-tRNA ligase /

glutamate-tRNA ligase activity / glutamyl-tRNA aminoacylation /

ATP binding /

cytoplasm
Similarity search - Function

Biological species

Plasmodium berghei ANKA (eukaryote)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.7 Å

Authors

Benas, P. / Jaramillo Ponce, J.R. / Frugier, M. / Sauter, C.

Funding support

France, 3items

Organization	Grant number	Country
Agence Nationale de la Recherche (ANR)	ANR-10-IDEX-0002	France
Agence Nationale de la Recherche (ANR)	ANR 20-SFRI-0012	France
Agence Nationale de la Recherche (ANR)	ANR-17-EURE-0023	France

Citation

Journal: Protein Sci. / Year: 2023
Title: Solution X-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes.
Authors: Jaramillo Ponce, J.R. / Theobald-Dietrich, A. / Benas, P. / Paulus, C. / Sauter, C. / Frugier, M.

History

Deposition	Oct 17, 2022	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Jan 25, 2023	Provider: repository / Type: Initial release
Revision 1.1	Feb 8, 2023	Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.title
Revision 1.2	Feb 7, 2024	Group: Data collection / Category: chem_comp_atom / chem_comp_bond

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8bcq.cif.gz	527.7 KB	Display	PDBx/mmCIF format
PDB format	pdb8bcq.ent.gz	369 KB	Display	PDB format
PDBx/mmJSON format	8bcq.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/bc/8bcq ftp://data.pdbj.org/pub/pdb/validation_reports/bc/8bcq	HTTPS FTP

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Related structure data

Related structure data	8bhdSC S: Starting model for refinement C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search
Other databases	SASBDB: SASDQ37N-terminal GST-like domain of Plasmodium berghei cytosolic glutamyl-tRNA synthetase SASBDB: SASDQ47N-terminal GST-like domain of Plasmodium berghei tRNA import protein SASBDB: SASDQ57The scaffold of Plasmodium berghei multi aminoacyl-tRNA synthetase complexes SASBDB: SASDQ67Plasmodium berghei multi aminoacyl-tRNA synthetase complex, Q-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and glutaminyl-tRNA synthetase SASBDB: SASDQ77Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (8) #212 - Aug 2017 Glutathione Transferases similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1)

Deposited unit

A: Glutamate--tRNA ligase

B: Glutamate--tRNA ligase

C: Glutamate--tRNA ligase

D: Glutamate--tRNA ligase

E: Glutamate--tRNA ligase

hetero molecules

151 kDa, 5 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Glutamate--tRNA ligase

hetero molecules

A: Glutamate--tRNA ligase

hetero molecules

defined by author&software
Evidence: gel filtration, see primary citation, light scattering, see primary citation, SAXS, see primary citation
61.3 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Glutamate--tRNA ligase

C: Glutamate--tRNA ligase

hetero molecules

defined by author&software
Evidence: gel filtration, see primary citation, light scattering, see primary citation
60.7 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

D: Glutamate--tRNA ligase

E: Glutamate--tRNA ligase

hetero molecules

defined by author&software
Evidence: gel filtration, see primary citation, light scattering, see primary citation
59.4 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

A: Glutamate--tRNA ligase

B: Glutamate--tRNA ligase

C: Glutamate--tRNA ligase

D: Glutamate--tRNA ligase

hetero molecules

defined by author
121 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

5

A: Glutamate--tRNA ligase

B: Glutamate--tRNA ligase

C: Glutamate--tRNA ligase

D: Glutamate--tRNA ligase

E: Glutamate--tRNA ligase

hetero molecules

A: Glutamate--tRNA ligase

hetero molecules

defined by author
181 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	129.979, 88.683, 169.284
Angle α, β, γ (deg.)	90.000, 106.130, 90.000
Int Tables number	5
Space group name H-M	C121
Space group name Hall	C2y
Symmetry operation	#1: x,y,z #2: -x,y,-z #3: x+1/2,y+1/2,z #4: -x+1/2,y+1/2,-z

Components on special symmetry positions

ID	Model	Components
1	1	A-401- HOH

#1: Protein	Glutamate--tRNA ligase Mass: 29647.863 Da / Num. of mol.: 5 / Mutation: A2, B2, C2, D2, E2: insertion (cloning artifact) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium berghei ANKA (eukaryote) / Strain: Anka / Gene: PBANKA_1362000 / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: A0A509AR09, glutamate-tRNA ligase
#2: Chemical	ChemComp-SO4 / SULFATE ION / Sulfate Mass: 96.063 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: SO₄
#3: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol Mass: 92.094 Da / Num. of mol.: 19 / Source method: obtained synthetically / Formula: C₃H₈O₃
#4: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.16 Å³/Da / Density % sol: 61.1 % Description: Small and fragile crystals initially diffracting only at 5 angstroms were obtained. Their diffraction limit was further improved up to 2.7 angstroms by microseeding.
Crystal grow	Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: Protein concentration: 6 to 9 mg/mL 0.1 M HEPS-NaOH pH 7.5, 1.4-1.5 M ammonium sulfate, 20% (v/v) glycerol and using microseeding. Temp details: use of incubators

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.97857 Å
Detector	Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 11, 2020 / Details: Kirkpatrick-Baez mirrors
Radiation	Monochromator: channel cut / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97857 Å / Relative weight: 1
Reflection	Resolution: 2.7→47.16 Å / Num. obs: 50722 / % possible obs: 99.69 % / Redundancy: 7 % / B_iso Wilson estimate: 82.29 Å² / CC_1/2: 0.999 / CC star: 1 / R_merge(I) obs: 0.07188 / R_pim(I) all: 0.02919 / R_rim(I) all: 0.07767 / Net I/σ(I): 14.81
Reflection shell	Resolution: 2.703→2.799 Å / Redundancy: 6.9 % / R_merge(I) obs: 1.181 / Mean I/σ(I) obs: 1.47 / Num. unique obs: 4911 / CC_1/2: 0.759 / CC star: 0.929 / R_pim(I) all: 0.4809 / R_rim(I) all: 1.277 / % possible all: 97.73

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Processing

Software

Name	Version	Classification
MxCuBE		data collection
XDS		data reduction
XSCALE		data scaling
PHENIX	dev_4701	phasing
PHENIX	dev_4701	refinement

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 8BHD

8bhd

Resolution: 2.7→47.16 Å / SU ML: 0.4038 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 26.4257
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2336	2535	5 %
R_work	0.1993	48153	-
obs	0.201	50688	99.71 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 121.4 Å²

Refinement step

Cycle: LAST / Resolution: 2.7→47.16 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	8433	0	154	16	8603

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0015	8753
X-RAY DIFFRACTION	f_angle_d	0.3761	11789
X-RAY DIFFRACTION	f_chiral_restr	0.0389	1287
X-RAY DIFFRACTION	f_plane_restr	0.0023	1443
X-RAY DIFFRACTION	f_dihedral_angle_d	11.726	3202

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.7-2.75	0.4248	126	0.4279	2518	X-RAY DIFFRACTION	95.87
2.75-2.81	0.3703	144	0.3297	2709	X-RAY DIFFRACTION	100
2.81-2.87	0.2726	149	0.2934	2615	X-RAY DIFFRACTION	99.93
2.87-2.94	0.3128	146	0.2681	2667	X-RAY DIFFRACTION	99.93
2.94-3.01	0.2613	144	0.2463	2667	X-RAY DIFFRACTION	99.89
3.01-3.09	0.262	129	0.2381	2682	X-RAY DIFFRACTION	99.96
3.09-3.18	0.3095	140	0.2473	2664	X-RAY DIFFRACTION	100
3.18-3.29	0.3234	139	0.2678	2694	X-RAY DIFFRACTION	99.82
3.29-3.4	0.2887	139	0.2312	2659	X-RAY DIFFRACTION	99.96
3.4-3.54	0.2409	140	0.2199	2680	X-RAY DIFFRACTION	100
3.54-3.7	0.2302	143	0.197	2673	X-RAY DIFFRACTION	99.96
3.7-3.9	0.2444	138	0.1846	2686	X-RAY DIFFRACTION	99.96
3.9-4.14	0.2068	145	0.1886	2692	X-RAY DIFFRACTION	100
4.14-4.46	0.1867	143	0.1671	2672	X-RAY DIFFRACTION	100
4.46-4.91	0.1879	138	0.164	2716	X-RAY DIFFRACTION	100
4.91-5.62	0.225	144	0.1841	2694	X-RAY DIFFRACTION	99.96
5.62-7.07	0.2496	142	0.2153	2706	X-RAY DIFFRACTION	100
7.08-47.16	0.2104	146	0.171	2759	X-RAY DIFFRACTION	99.49

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.84574803751	0.401834615205	-0.398223059258	0.966506300121	0.0735932460347	3.5572524801	0.159525326831	-0.181362955269	-0.285955472785	-0.0296112625606	-0.103595594776	0.0322539840075	0.205473763786	0.245784913949	-2.06370133532E-5	0.57860481603	-0.0420505281362	-0.0590749576602	0.498248004744	0.103892336024	0.772909106102	43.0695275833	21.2771911362	94.2548005406
2	3.41100367779	-0.646124347187	-0.243807002381	1.48141588141	0.0173298955042	0.610969744743	0.137268736969	-0.223813537782	0.16883706868	-0.00567599046405	-0.0702986149739	0.00596215240506	-0.136880947739	-0.188461615942	6.18814366074E-5	0.584231909795	-0.0844856210818	0.0667652909931	0.529792802534	0.00700123343555	0.633099982706	27.0632131059	30.9449783734	92.9841777925
3	0.244892425256	-0.611846645066	0.268426286325	2.23792931773	-0.177029103886	2.21012170519	0.14874343408	-0.165436600416	-0.14941880365	-0.00880536267243	-0.0992754965802	0.333787172581	0.248919630565	-0.048717894744	3.03804508432E-6	0.56655043692	0.0585518603526	-0.00943360759579	0.567275331381	-0.00739346639277	0.744848010574	11.5313729342	39.2961629373	69.7746879857
4	0.35891454206	0.103102308631	-0.06711503211	-0.00263844332015	0.0163705990305	-0.0130243047151	0.558217931823	0.0271130401979	0.746091363903	-0.695655724733	-0.470384970749	0.287516533207	-0.330578806787	-0.385412153984	-0.000692284472071	0.740339661557	0.258455861453	-0.149857113495	0.822391072645	-0.0105997142465	1.13664673123	-0.566329822352	52.5482826638	57.2515668829
5	0.391012236035	-0.454334331175	0.0723809411939	0.267338048572	0.40641439716	0.739219389154	-0.0120192895977	0.647682650761	0.317816856577	-0.55775352936	0.0951002047807	-0.556135080245	-0.224763609962	0.900401735636	5.45158723726E-5	0.578568438593	0.107285946826	0.0537775250378	0.672777911683	0.0299389064593	0.969379058057	17.075583706	60.9729910836	64.3002547402
6	0.713554796339	-1.37910853248	-0.803290416624	2.56211620239	1.27505068801	0.895675424205	-0.26711719139	-0.258653103045	0.438349720527	0.381647247769	0.0871636927616	-0.188482610154	-0.104810376125	-0.00307716594313	2.19994142353E-5	0.590209554825	0.0887969452789	0.0087838037728	0.617828355062	-0.0320400422041	0.815534047204	10.4712406198	59.27625883	76.5956651965
7	0.0982103522925	0.239062923409	-0.439093644949	0.756859252242	-0.64396421014	0.924078755537	0.256717794205	1.05316718024	0.836076194239	-0.906757721216	-0.0406540929114	0.104488903752	-0.513631580722	-0.165590925041	0.00220888243652	1.06352448307	0.260368743734	0.0771835959137	1.04727885895	0.297114365536	1.01658382084	15.485455765	56.3633522918	40.83109475
8	1.13710094281	-0.0315684257959	-0.84913539551	0.209379618369	-0.0493872012106	0.494614489655	0.364406596189	0.914605104979	0.111983056787	-0.0688168849278	-0.366847679908	0.692773134435	0.19441797988	-0.131019804421	-2.70174195786E-5	0.789868964479	0.242844070601	-0.0816159254226	0.944754867825	0.0482814361062	0.878926807301	10.5992165263	46.2370122018	49.6327166714
9	1.54627130279	-0.248707497644	-0.912946946749	1.00969719093	0.470427960295	1.90504548915	0.427431468112	0.161019919016	0.0787450637992	-0.228906765635	-0.162133920561	-0.188683248527	0.275632192407	0.338392902456	0.0323956546443	0.7683340711	0.33959392434	0.0686690329257	0.916675278946	0.0133328680675	0.609010095099	28.4093800016	40.8020754742	44.9519691276
10	1.20955815298	0.694021216484	-0.314342411108	0.552424456278	-0.470135751744	0.00751851443076	0.185837513217	-0.067179134209	0.0577701441333	0.604243828237	0.297790026291	0.181247624991	0.488693888112	-0.164302190663	5.52718095825E-5	1.61992484557	-0.0437047959148	-0.244901468576	1.12357023485	0.084808923656	1.0099421232	3.17838019562	11.287238665	32.0856332727
11	1.46033337452	1.16719172519	-0.28902033423	1.17310187926	-0.418936314492	0.235107088177	0.374921290798	0.981285256463	0.743693531051	-0.299266230107	0.416829854999	0.212937682297	1.13370299948	1.06119995321	0.0770762551741	1.47799143501	0.281491099894	-0.161040641969	1.42710702877	-0.0322048063171	1.0263636208	16.5167215544	23.7987930082	25.1377495831
12	0.82247927569	0.134814327707	-0.244854589801	0.902143900334	0.984860358375	1.17680440248	0.476554125252	0.324342663741	-0.175920685003	0.214881396424	-0.246893551434	-0.729617845135	0.884767451524	0.628666350105	0.00125527659737	1.64312234607	0.362455008547	-0.24259253586	1.08193622487	-0.0653817441971	0.721260232807	22.0751534169	14.7719834989	41.0431730135
13	-0.0594742684146	-0.00322300174136	-0.00429201804973	0.0218889528669	-0.0192932885414	-0.0666220652674	0.777730452924	0.815518965807	0.131872006475	-0.611880431328	0.25099809986	-0.892889805999	-0.544959550327	0.130805277843	0.000165706569784	1.63854099838	0.354110786745	-0.0930212788165	2.11130369689	-0.377421325961	1.21261319626	28.1242709754	18.1045678619	8.40216649683
14	2.44868484154	1.28360737527	-0.797435415712	0.649873065955	-0.386023046819	0.218557052879	0.10095409117	0.704529802077	0.931777601026	0.0380256107423	0.742037741202	-0.0282540343897	0.38031715887	0.749211162206	0.160828572916	2.11453332585	0.222828580119	-0.323408698937	1.94127669249	-0.117946629263	1.04049775247	14.7315761582	22.6370642788	2.41283149159
15	0.119370174322	0.231244442518	0.259397597674	0.287662683185	0.257321147625	0.0716086143837	-0.0494871096697	0.842739182424	-0.540327789973	0.435133338177	0.683045311185	0.774907165752	0.266003443393	0.427814328508	-0.000146198112282	1.8090289153	0.27018208349	-0.0590896569804	1.98154988236	0.0459036821896	1.31917558132	24.357581556	22.2288657788	6.74384910748
16	-0.00970800697835	0.0243801832454	-0.0445401757472	0.106570349542	0.0785997843347	0.09548066378	0.952429219961	-0.203887067887	0.355305408084	-0.904759890032	-0.546922053029	0.32592305073	0.143899746801	0.281015174501	4.69221249033E-5	1.68176105965	0.212650624086	-0.123067079421	1.68068100082	0.00642889820943	0.927630376342	21.9536231588	20.4050571235	15.4654142563
17	0.0115546658014	0.0114111809241	-0.0103377197658	-0.0712283440857	0.0451328417927	0.030776235438	-0.431861086487	0.643603204594	0.766992640544	-0.458004574893	-0.550267552897	-0.0782795382842	-0.246181313472	-0.502578480787	-0.000208594350792	1.70534344408	0.292999287642	-0.459653584447	1.52078760835	-0.0554132072191	1.08994613566	4.66648397939	20.0178075998	12.9357729752
18	0.0667504995478	0.0446867083605	-0.132471967306	0.0267432819933	-0.102649646455	0.0864578320389	0.000213994865002	0.0793876447181	-0.841555604008	0.693778715892	0.243375719778	0.604033236111	0.399880463463	-0.763767582965	0.000111495603738	2.19385787873	0.0919038377846	-0.389112924994	1.48839140741	-0.0355198232434	1.28276069185	6.25784183924	9.34555591855	17.4004561691
19	0.00731734858075	-0.0467395187073	0.00381093274475	0.0688178669495	-0.0526132438756	0.011564905395	-1.16477734431	0.0790124496125	-0.756646821789	-0.19085349564	1.1602686611	0.469452355638	-0.0386663858884	0.0314454780072	0.000152499709665	2.39662458893	0.364180932983	-0.246694120674	1.9730796892	-0.461121598748	1.41475916985	13.7787555863	-4.38487775524	9.92639064313
20	0.028121478921	0.0556297956761	0.040858019437	0.0449683785142	0.0364725187432	0.0153686988942	0.126398232254	-0.337766810432	-0.0964952553082	-0.183448153276	0.00206567666774	0.143673595952	0.790129024227	-0.38279069273	6.79084719417E-5	2.50705093989	0.0338696459452	-0.387779586033	1.62932122939	0.0203256000451	0.872448963902	1.37586510131	7.91617142021	7.19189687317
21	0.134194832466	0.0298062603063	-0.06838482865	0.0529852218243	-0.0505299640482	0.0111833280722	-0.620698346974	0.918334165139	-0.759766842037	0.413417020412	0.251373218105	-0.17727656912	-0.270459497941	-0.220415991503	-0.00450905015244	2.57186384106	0.291527362342	-0.338144441127	1.77130165851	-0.326829984509	1.12452146088	16.2405354135	3.8789769239	2.44136383528
22	-0.0108648693824	0.000335971849494	-0.00035520386929	-0.011227502198	0.00283455164395	-0.00241985847054	0.322748799671	-0.455508017113	0.273008593586	-0.106264031941	-0.472966797996	-0.280442116032	-0.0652196694342	0.0717997438486	-5.62011822769E-5	3.38633398465	-0.0599089216043	-0.639843230936	1.9741362229	-0.376529600341	1.52902094196	15.6352309443	-7.6289996925	-3.15598251817
23	1.09829803774	0.484277183682	-0.336623910793	0.258900978074	-0.128958906386	0.0709589874543	-0.842707045087	0.101653983899	-0.539473747147	0.194148912705	0.239364792591	-0.276227613601	-0.106880266447	0.392194654669	-0.0585012973259	2.54691613982	0.447660549298	-0.359798304891	2.10411023365	-0.477514892601	1.0297693163	7.65248802676	3.27228045965	-1.91699922128
24	1.86573420297	2.42415626692	0.214791040014	3.20719579162	0.288923494376	0.0151053688512	-0.493633823418	0.547896390082	1.05414589474	-0.210517749475	0.218364488398	1.20980797997	-0.076358478114	-0.551522395919	-0.00807257510144	1.97484143382	-0.201966516991	0.140151874276	2.53688029016	-0.111961907596	0.228893941058	20.8529239274	14.0693407289	-4.14465150555

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 5 through 116 )	A	A	5 - 116	1 - 86
2	2	chain 'A' and (resid 117 through 227 )	A	A	117 - 227	87 - 197
3	3	chain 'B' and (resid 5 through 99 )	B	B	5 - 99	1 - 75
4	4	chain 'B' and (resid 100 through 116 )	B	B	100 - 116	76 - 92
5	5	chain 'B' and (resid 117 through 153 )	B	B	117 - 153	93 - 129
6	6	chain 'B' and (resid 154 through 227 )	B	B	154 - 227	130 - 203
7	7	chain 'C' and (resid 6 through 66 )	C	C	6 - 66	1 - 52
8	8	chain 'C' and (resid 67 through 116 )	C	C	67 - 116	53 - 87
9	9	chain 'C' and (resid 117 through 227 )	C	C	117 - 227	88 - 198
10	10	chain 'D' and (resid 7 through 99 )	D	D	7 - 99	1 - 72
11	11	chain 'D' and (resid 100 through 134 )	D	D	100 - 134	73 - 104
12	12	chain 'D' and (resid 135 through 226 )	D	D	135 - 226	105 - 196
13	13	chain 'E' and (resid 7 through 17 )	E	E	7 - 17	1 - 11
14	14	chain 'E' and (resid 18 through 34 )	E	E	18 - 34	12 - 28
15	15	chain 'E' and (resid 35 through 60 )	E	E	35 - 60	29 - 47
16	16	chain 'E' and (resid 61 through 99 )	E	E	61 - 99	48 - 72
17	17	chain 'E' and (resid 100 through 115 )	E	E	100 - 115	73 - 82
18	18	chain 'E' and (resid 116 through 133 )	E	E	116 - 133	83 - 100
19	19	chain 'E' and (resid 134 through 155 )	E	E	134 - 155	101 - 122
20	20	chain 'E' and (resid 156 through 166 )	E	E	156 - 166	123 - 133
21	21	chain 'E' and (resid 167 through 184 )	E	E	167 - 184	134 - 151
22	22	chain 'E' and (resid 185 through 194 )	E	E	185 - 194	152 - 161
23	23	chain 'E' and (resid 195 through 207 )	E	E	195 - 207	162 - 174
24	24	chain 'E' and (resid 208 through 224 )	E	E	208 - 224	175 - 191

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