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- PDB-8b5e: Exploring the ligand binding and conformational dynamics of recep... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8b5e | ||||||
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Title | Exploring the ligand binding and conformational dynamics of receptor domain 1 of the ABC transporter GlnPQ | ||||||
![]() | ABC transporter permease subunit | ||||||
![]() | TRANSPORT PROTEIN / Transporter / membrane protein | ||||||
Function / homology | ![]() peptide transport / ligand-gated monoatomic ion channel activity / ATP-binding cassette (ABC) transporter complex / protein transport Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Whittaker, J. / Guskov, A. | ||||||
Funding support | 1items
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![]() | ![]() Title: Exploring the ligand binding and conformational dynamics of receptor domain 1 of the ABC transporter GlnPQ Authors: Whittaker, J. / Guskov, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 370 KB | Display | ![]() |
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PDB format | ![]() | 304.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 21.5 KB | Display | |
Data in CIF | ![]() | 31.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8b5dC ![]() 6h30S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 24519.641 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: glnP, AMHIJAGA_02474, GII02_01815 / Production host: ![]() ![]() |
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-Non-polymers , 6 types, 271 molecules ![](data/chem/img/ARG.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ChemComp-NA / | #4: Chemical | ChemComp-GOL / | #5: Chemical | ChemComp-PEG / #6: Chemical | ChemComp-PGE / | #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.16 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 100 mM NaCl 50 mM TRIS 10 % MPD 15 % PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 22, 2020 |
Radiation | Monochromator: Cu / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→53.98 Å / Num. obs: 50688 / % possible obs: 99 % / Redundancy: 6 % / CC1/2: 0.96 / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.6→3.5 Å / Num. unique obs: 49269 / CC1/2: 0.94 / % possible all: 93 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6H30 Resolution: 1.6→38.66 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso max: 112.37 Å2 / Biso mean: 27.05 Å2 / Biso min: 12.75 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→38.66 Å
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