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- PDB-8b0v: Crystal structure of C-terminal domain of Pseudomonas aeruginosa ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8b0v | ||||||
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Title | Crystal structure of C-terminal domain of Pseudomonas aeruginosa LexA G91D mutant | ||||||
![]() | LexA repressor | ||||||
![]() | TRANSCRIPTION / transcriptional regulator / serine-lysine protease | ||||||
Function / homology | ![]() repressor LexA / SOS response / DNA replication / serine-type endopeptidase activity / DNA repair / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / proteolysis / DNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vascon, F. / De Felice, S. / Chinellato, M. / Maso, L. / Cendron, L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural investigations on the SOS response in Pseudomonas aeruginosa Authors: Vascon, F. / De Felice, S. / Gasparotto, M. / Huber, S.T. / Catalano, C. / Chinellato, M. / Grinzato, A. / Filippini, F. / Maso, L. / Jakobi, A. / Cendron, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 64.3 KB | Display | ![]() |
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PDB format | ![]() | 46.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 459.4 KB | Display | ![]() |
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Full document | ![]() | 461.5 KB | Display | |
Data in XML | ![]() | 12.3 KB | Display | |
Data in CIF | ![]() | 15.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1jhfS ![]() 15038 ![]() 7zzm S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13849.001 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.29 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2M CaCl2, 0.1M MES pH 6.0, 20%w/v PEG 6000, 2.5 mM Tb-Xo4 (Crystallophore 1) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 10, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→38.92 Å / Num. obs: 25087 / % possible obs: 99.8 % / Redundancy: 5.1 % / CC1/2: 0.998 / Rmerge(I) obs: 0.072 / Net I/σ(I): 9.2 |
Reflection shell | Resolution: 1.7→1.73 Å / Rmerge(I) obs: 1.072 / Num. unique obs: 1331 / CC1/2: 0.721 / % possible all: 99.95 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 1JHF Resolution: 1.7→38.92 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 88.53 Å2 / Biso mean: 33.6902 Å2 / Biso min: 16.91 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→38.92 Å
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LS refinement shell | Resolution: 1.7→1.73 Å /
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