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Open data
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Basic information
Entry | Database: PDB / ID: 8ad6 | ||||||
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Title | Structure of DarB bound to c-di-AMP | ||||||
![]() | CBS domain-containing protein | ||||||
![]() | SIGNALING PROTEIN / c-di-AMP / potassium stress / ppGpp / Rel / stringent factor / CbpB | ||||||
Function / homology | CBS domain superfamily / Chem-2BA / CBS domain-containing protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Garcia-Pino, A. / Talavera, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of DarB bound to c-di-AMP Authors: Garcia-Pino, A. / Talavera, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.2 KB | Display | ![]() |
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PDB format | ![]() | 55.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 14.9 KB | Display | |
Data in CIF | ![]() | 21.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6yj8S S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16653.346 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() References: UniProt: A0A164SLA6 #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.54 % |
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Crystal grow | Temperature: 293.16 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 0.2 M Lithium sulfate 0.1 M MES 6.0 20 % w/v PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 26, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9801 Å / Relative weight: 1 |
Reflection | Resolution: 1.52→52.81 Å / Num. obs: 40317 / % possible obs: 88 % / Redundancy: 7.3 % / CC1/2: 0.999 / Rpim(I) all: 0.021 / Net I/σ(I): 19.2 |
Reflection shell | Resolution: 1.52→1.57 Å / Num. unique obs: 998 / CC1/2: 0.826 / Rpim(I) all: 0.251 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6YJ8 Resolution: 1.52→52.81 Å / Cross valid method: THROUGHOUT Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 22.73 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.52→52.81 Å
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Refine LS restraints |
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