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Yorodumi- PDB-8ab4: Crystal structure of MreB from Geobacillus stearothermophilus ATC... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8ab4 | ||||||
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Title | Crystal structure of MreB from Geobacillus stearothermophilus ATCC7953 in complex with GTP | ||||||
Components | Cell shape-determining protein MreB | ||||||
Keywords | STRUCTURAL PROTEIN / BACTERIAL ACTIN / BACTERIAL CYTOSKELETON | ||||||
Function / homology | Cell shape determining protein MreB / MreB/Mbl protein / cell morphogenesis / ATPase, nucleotide binding domain / regulation of cell shape / ATP binding / cytoplasm / GUANOSINE-5'-TRIPHOSPHATE / Cell shape-determining protein MreB Function and homology information | ||||||
Biological species | Geobacillus stearothermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.22 Å | ||||||
Authors | Li de la Sierra-Gallay, I. | ||||||
Funding support | France, 1items
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Citation | Journal: To Be Published Title: Polymerization cycle of an actin homolog MreB from a Gram-positive bacterium Authors: Mao, W. / Conilleau, C. / Li de la Sierra-Gallay, I. / Ah-Seng, Y. / van Tilbeurgh, H. / Renner, L. / Nessler, S. / Bertin, A. / Chastanet, A. / Carballido-Lopes, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ab4.cif.gz | 74.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ab4.ent.gz | 52.6 KB | Display | PDB format |
PDBx/mmJSON format | 8ab4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ab/8ab4 ftp://data.pdbj.org/pub/pdb/validation_reports/ab/8ab4 | HTTPS FTP |
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-Related structure data
Related structure data | 8aamC 7zptS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36445.008 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Geobacillus stearothermophilus (bacteria) Gene: mreB, B4109_2230, B4114_2117, GS458_2390 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A150MJ77 |
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#2: Chemical | ChemComp-GTP / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.84 Å3/Da / Density % sol: 33.06 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / pH: 5.5 / Details: PEG 300, 2-(N-morpholino)ethanesulfonic acid |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.978565 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 9, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978565 Å / Relative weight: 1 |
Reflection | Resolution: 2.22→47.27 Å / Num. obs: 13182 / % possible obs: 99.5 % / Redundancy: 6.9 % / Biso Wilson estimate: 53.43 Å2 / CC1/2: 1 / Rrim(I) all: 0.09 / Net I/σ(I): 12.74 |
Reflection shell | Resolution: 2.22→2.35 Å / Num. unique obs: 2073 / CC1/2: 0.6 / Rrim(I) all: 1.61 / % possible all: 98.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7ZPT Resolution: 2.22→42.83 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 33.37 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 113.8 Å2 / Biso mean: 64.0333 Å2 / Biso min: 40.23 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.22→42.83 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 5
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