+Open data
-Basic information
Entry | Database: PDB / ID: 8a7z | ||||||
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Title | NMR structure of holo-acp | ||||||
Components | Hybrid polyketide synthase-non ribosomal peptide synthetase | ||||||
Keywords | TRANSFERASE / Acyl carrier protein | ||||||
Function / homology | Function and homology information toxin biosynthetic process / amide biosynthetic process / : / DIM/DIP cell wall layer assembly / fatty acid synthase activity / phosphopantetheine binding / 3-oxoacyl-[acyl-carrier-protein] synthase activity / antibiotic biosynthetic process / fatty acid biosynthetic process / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Streptomyces virginiae (bacteria) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Collin, S. / Weissman, K.J. / Chagot, B. / Gruez, A. | ||||||
Funding support | France, 1items
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Citation | Journal: Nat Commun / Year: 2023 Title: Decrypting the programming of beta-methylation in virginiamycin M biosynthesis. Authors: Collin, S. / Cox, R.J. / Paris, C. / Jacob, C. / Chagot, B. / Weissman, K.J. / Gruez, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8a7z.cif.gz | 498.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8a7z.ent.gz | 420.1 KB | Display | PDB format |
PDBx/mmJSON format | 8a7z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a7/8a7z ftp://data.pdbj.org/pub/pdb/validation_reports/a7/8a7z | HTTPS FTP |
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-Related structure data
Related structure data | 8ahqC 8ahzC 8aigC 8allC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8882.927 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces virginiae (bacteria) / Gene: virA / Plasmid: pBG102 / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: A4PHN0 |
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#2: Chemical | ChemComp-PNS / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 1.0 mM [U-13C; U-15N] acp5a-holo, 100 mM sodium phosphate, 0.5 mM TCEP, 90% H2O/10% D2O Label: sample1 / Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Details: 100 mM NaP / Ionic strength: 100 mM / Label: condition_1 / pH: 6 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 4 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 20 |