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- PDB-7zs1: Diheme cytochrome c Kustd1711 from Kuenenia stuttgartiensis, M292... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7zs1 | ||||||
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Title | Diheme cytochrome c Kustd1711 from Kuenenia stuttgartiensis, M292C mutant | ||||||
![]() | Hypothetical (Diheme) protein | ||||||
![]() | ELECTRON TRANSPORT / anaerobic ammonium oxidation | ||||||
Function / homology | ![]() electron transfer activity / heme binding / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Akram, M. / Barends, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Redox potential tuning by calcium ions in a novel c-type cytochrome from an anammox organism Authors: Akram, M. / Barends, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 91.7 KB | Display | ![]() |
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PDB format | ![]() | 55.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.5 MB | Display | ![]() |
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Full document | ![]() | 2.5 MB | Display | |
Data in XML | ![]() | 14.7 KB | Display | |
Data in CIF | ![]() | 20.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7zs0C ![]() 7zs2C C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 34267.113 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() | ||||||||
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#2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.2 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.2 M calcium acetate, 0.1 M imidazole/HCl pH 8.0 and 20% PEG 1K |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 1, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99988 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→50 Å / Num. obs: 35153 / % possible obs: 99.5 % / Redundancy: 4 % / Biso Wilson estimate: 30.11 Å2 / Rpim(I) all: 0.047 / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.7→1.74 Å / Mean I/σ(I) obs: 1.5 / Num. unique obs: 2567 / Rpim(I) all: 1.057 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: WT Structure Resolution: 1.7→47.57 Å / SU ML: 0.212 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 23.9863 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.11 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→47.57 Å
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Refine LS restraints |
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LS refinement shell |
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