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Open data
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Basic information
Entry | Database: PDB / ID: 7zq7 | ||||||
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Title | Structure of RpF-1 | ||||||
![]() | Crotonase/enoyl-CoA hydratase family protein | ||||||
![]() | LIPID TRANSPORT / Enoyl coA Hydratase / lipid / pathogenic regulation | ||||||
Function / homology | enoyl-CoA hydratase / Enoyl-CoA hydratase/isomerase / Enoyl-CoA hydratase/isomerase / ClpP/crotonase-like domain superfamily / lyase activity / Crotonase/enoyl-CoA hydratase family protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sanchez-Alba, L. / Reverter, D. / Conchillo, O. / Yero, D. / Daura, X. / Gibert, I. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of RpF-1 Authors: Sanchez-Alba, L. / Reverter, D. / Conchillo, O. / Yero, D. / Daura, X. / Gibert, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 311.3 KB | Display | ![]() |
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PDB format | ![]() | 254.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 486.1 KB | Display | ![]() |
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Full document | ![]() | 541.5 KB | Display | |
Data in XML | ![]() | 59.8 KB | Display | |
Data in CIF | ![]() | 80.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3m6nS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33910.848 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: rpfF, B9Y74_06275, DUW70_13565, FEO86_10500, FEO88_11330, FEO89_10240, FLFIOBJN_02527, HKK60_11705, WP1W18C01_20950 Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.21 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / Details: 12% 2-propanol, 100mM HEPES, 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 28, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 2.99→81.37 Å / Num. obs: 38043 / % possible obs: 99.5 % / Redundancy: 3.4 % / CC1/2: 0.97 / Net I/σ(I): 5.2 |
Reflection shell | Resolution: 2.99→3.047 Å / Num. unique obs: 1888 / CC1/2: 0.63 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3M6N Resolution: 3→81.37 Å / SU ML: 0.53 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 32.35 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 138.34 Å2 / Biso mean: 71.8003 Å2 / Biso min: 26.69 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3→81.37 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 13
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