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- PDB-7zp0: Crystal structure of CusS histidine kinase catalytic core from Es... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7zp0 | ||||||
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Title | Crystal structure of CusS histidine kinase catalytic core from Escherichia coli | ||||||
![]() | Sensor protein | ||||||
![]() | TRANSFERASE / histidine kinase / two-component signal transduction system / copper resistance | ||||||
Function / homology | ![]() histidine kinase / phosphorelay sensor kinase activity / membrane => GO:0016020 / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli 'BL21-GoldpLysS AG' | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cociurovscaia, A. / Bujacz, G. / Pietrzyk-Brzezinska, A. | ||||||
Funding support | 1items
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![]() | ![]() Title: Crystal structure of the Escherichia coli CusS kinase core. Authors: Cociurovscaia, A. / Bujacz, G. / Pietrzyk-Brzezinska, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 533 KB | Display | ![]() |
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PDB format | ![]() | 439.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 484.4 KB | Display | ![]() |
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Full document | ![]() | 498.9 KB | Display | |
Data in XML | ![]() | 60.1 KB | Display | |
Data in CIF | ![]() | 88.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2c2aS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23953.525 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: First four amino acid residues at the N-terminus (GAMA) were retained after tag cleavage. The protein represents the fragment of CusS involving the residues 268-480. Source: (gene. exp.) ![]() ![]() Strain: DE3 / Gene: cusS, CWS33_25405, GNZ05_00640, NCTC8985_02504 Production host: ![]() ![]() References: UniProt: A0A2J1D710, histidine kinase #2: Chemical | ChemComp-EDO / #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.74 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 7.5 % Tacsimate pH 7.0, 0.1 M HEPES pH 7.0, 10 % mmePEG 2000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jun 11, 2021 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9184 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.398→36.628 Å / Num. obs: 240118 / % possible obs: 98.9 % / Redundancy: 6.75 % / Biso Wilson estimate: 20.24 Å2 / CC1/2: 0.99 / Net I/σ(I): 15.48 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2c2a Resolution: 1.398→36.628 Å / SU ML: 0.18 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 23.89 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 200.88 Å2 / Biso mean: 36.8444 Å2 / Biso min: 11.68 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.398→36.628 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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