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Yorodumi- PDB-7zok: A novel molecular switch controls assembly of bacterial focal adh... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7zok | ||||||
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Title | A novel molecular switch controls assembly of bacterial focal adhesions in response to changes in surface structure. | ||||||
Components | Adventurous gliding motility protein GltJ | ||||||
Keywords | CELL ADHESION / protein / motility / GYF domain / myxococcus xanthus / adventurous motility / gliding / focal adhesion complex / adhesion / regulatory domain / myxobacteria | ||||||
Function / homology | Zinc finger/thioredoxin putative / zinc-ribbon domain / GYF domain 2 / GYF domain 2 / Adventurous gliding motility protein GltJ Function and homology information | ||||||
Biological species | Myxococcus xanthus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Attia, B. / My, L. / Castaing, J.P. / Le Guenno, H. / Espinosa, L. / Schmidt, V. / Nouailler, M. / Bornet, O. / Mignot, T. / Elantak, L. | ||||||
Funding support | France, 1items
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Citation | Journal: To Be Published Title: A novel molecular switch controls assembly of bacterial focal adhesions in response to changes in surface structure. Authors: Attia, B. / My, L. / Castaing, J.P. / Le Guenno, H. / Espinosa, L. / Schmidt, V. / Nouailler, M. / Bornet, O. / Elantak, L. / Mignot, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7zok.cif.gz | 317 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7zok.ent.gz | 265.7 KB | Display | PDB format |
PDBx/mmJSON format | 7zok.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7zok_validation.pdf.gz | 405 KB | Display | wwPDB validaton report |
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Full document | 7zok_full_validation.pdf.gz | 511.6 KB | Display | |
Data in XML | 7zok_validation.xml.gz | 18.7 KB | Display | |
Data in CIF | 7zok_validation.cif.gz | 31.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zo/7zok ftp://data.pdbj.org/pub/pdb/validation_reports/zo/7zok | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5779.799 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Myxococcus xanthus (bacteria) / Gene: gltJ, HNV27_16755 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A7Y4JDV0 |
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#2: Chemical | ChemComp-ZN / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 0.45 mM [U-100% 13C; U-100% 15N] ZnR, 90% H2O/10% D2O Details: ZnR cytosolic domain of the GltJ protein / Label: 15N_13C / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.45 mM / Component: ZnR / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 10 mM / Ionic strength err: 0.1 / Label: c1 / pH: 7 / PH err: 0.1 / Pressure: 1 atm / Pressure err: 0.01 / Temperature: 303 K / Temperature err: 0.1 |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE II / Manufacturer: Bruker / Model: AVANCE II / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 4 | |||||||||||||||
NMR representative | Selection criteria: target function | |||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 20 / Conformers submitted total number: 20 |