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- PDB-7zhc: Moss spermine/spermidine acetyl transferase (PpSSAT) in complex w... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7zhc | ||||||
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Title | Moss spermine/spermidine acetyl transferase (PpSSAT) in complex with AcetylCoA and polyethylen glycol | ||||||
![]() | N-acetyltransferase domain-containing protein | ||||||
![]() | TRANSFERASE / SSAT / COENZYME A / ACYLTRANSFERASE | ||||||
Function / homology | N-acetyltransferase activity / Acetyltransferase (GNAT) family / Gcn5-related N-acetyltransferase (GNAT) domain profile. / GNAT domain / Acyl-CoA N-acyltransferase / ACETYL COENZYME *A / N-acetyltransferase domain-containing protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Morera, S. / Kopecny, D. / Vigouroux, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Biochemical and structural basis of polyamine, lysine and ornithine acetylation catalyzed by spermine/spermidine N-acetyl transferase in moss and maize. Authors: Belicek, J. / Luptakova, E. / Kopecny, D. / Frommel, J. / Vigouroux, A. / Cavar Zeljkovic, S. / Jagic, F. / Briozzo, P. / Kopecny, D.J. / Tarkowski, P. / Nisler, J. / De Diego, N. / Morera, S. / Kopecna, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 178.4 KB | Display | ![]() |
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PDB format | ![]() | 140.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 19.3 KB | Display | |
Data in CIF | ![]() | 26.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7zktC ![]() 2beiS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26103.910 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: A0A2K1KKM6 #2: Chemical | #3: Chemical | ChemComp-GOL / | #4: Chemical | ChemComp-1PE / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.53 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / Details: 10% PEG 8000, 10% PEG 1000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 26, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99187 Å / Relative weight: 1 |
Reflection | Resolution: 1.819→48.89 Å / Num. obs: 23866 / % possible obs: 92.1 % / Redundancy: 6.4 % / Biso Wilson estimate: 35.59 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.102 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 1.819→2.05 Å / Rmerge(I) obs: 1.138 / Num. unique obs: 1194 / CC1/2: 0.497 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2BEI Resolution: 1.819→48.89 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.928 / SU R Cruickshank DPI: 0.237 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.238 / SU Rfree Blow DPI: 0.181 / SU Rfree Cruickshank DPI: 0.183
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Displacement parameters | Biso max: 81.17 Å2 / Biso mean: 42.44 Å2 / Biso min: 19.39 Å2
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Refine analyze | Luzzati coordinate error obs: 0.26 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.819→48.89 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.82→1.98 Å / Rfactor Rfree error: 0 / Total num. of bins used: 51
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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