+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 7zg6 | |||||||||||||||
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タイトル | TacA1 antitoxin | |||||||||||||||
要素 | DUF1778 domain-containing protein | |||||||||||||||
キーワード | ANTITOXIN / Salmonella / Toxin-antitoxin system / RHH domain | |||||||||||||||
機能・相同性 | Vibrio phage ICP1, Orf50 / Protein of unknown function (DUF1778) / Ribbon-helix-helix / regulation of DNA-templated transcription / DUF1778 domain-containing protein 機能・相同性情報 | |||||||||||||||
生物種 | Salmonella enterica subsp. enterica serovar Typhimurium (サルモネラ菌) | |||||||||||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 1.95 Å | |||||||||||||||
データ登録者 | Grabe, G.J. / Morgan, R.M.L. / Helaine, S. | |||||||||||||||
資金援助 | 英国, European Union, 4件
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引用 | ジャーナル: Nat.Struct.Mol.Biol. / 年: 2024 タイトル: Molecular stripping underpins derepression of a toxin-antitoxin system. 著者: Grabe, G.J. / Giorgio, R.T. / Wieczor, M. / Gollan, B. / Sargen, M. / Orozco, M. / Hare, S.A. / Helaine, S. | |||||||||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7zg6.cif.gz | 42.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7zg6.ent.gz | 29.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 7zg6.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 7zg6_validation.pdf.gz | 429.7 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 7zg6_full_validation.pdf.gz | 430.8 KB | 表示 | |
XML形式データ | 7zg6_validation.xml.gz | 6.7 KB | 表示 | |
CIF形式データ | 7zg6_validation.cif.gz | 8.3 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/zg/7zg6 ftp://data.pdbj.org/pub/pdb/validation_reports/zg/7zg6 | HTTPS FTP |
-関連構造データ
関連構造データ | 7zg5C 7ak7S S: 精密化の開始モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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Components on special symmetry positions |
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-要素
#1: タンパク質 | 分子量: 10429.851 Da / 分子数: 2 / 由来タイプ: 組換発現 由来: (組換発現) Salmonella enterica subsp. enterica serovar Typhimurium (サルモネラ菌) 遺伝子: A3104_05255, A3S30_03565, A3T81_04105, A3U32_05670, A3V03_13915, A3V89_00845, A3W57_03990, A3W75_07045, A3X15_00845, A3X55_00325, A3Y76_07970, A4N07_08005, A4O05_01325, A4O41_02030, A4R48_ ...遺伝子: A3104_05255, A3S30_03565, A3T81_04105, A3U32_05670, A3V03_13915, A3V89_00845, A3W57_03990, A3W75_07045, A3X15_00845, A3X55_00325, A3Y76_07970, A4N07_08005, A4O05_01325, A4O41_02030, A4R48_08935, A6D61_12035, AAA76_07485, AAB27_15580, AAB79_05115, AAC35_03625, ADQ28_10175, AF497_21965, AGM99_05600, AHN93_12130, AIT36_00965, AKH62_19105, AL144_05695, AL168_00235, AL184_01805, AQ530_13115, AU613_00250, AVA38_12925, AVC05_10875, AVL16_07530, AWT30_08430, AXX99_15530, B1265_05360, B1398_03145, B1642_07215, B1P38_10085, B2E31_12120, B4V59_11835, B4W90_05735, B6362_03215, B7Q27_05355, B8Y16_03500, B8Z46_06180, B9C90_03095, B9C96_10075, B9M14_05165, B9O84_11710, BBQ66_14315, BG493_00970, BIC00_08660, BIC13_03220, BK110_05555, BKM50_23075, BMS46_12650, BMU56_14435, BZ203_17650, BZZ88_00325, C5W43_16875, CA117_15910, CAC56_00970, CAC59_00965, CB102_05780, CB119_09125, CB198_11360, CB380_04130, CB535_00770, CB570_01825, CB646_00870, CBM67_04325, CBM76_05660, CBZ90_17945, CC339_13495, CC403_04170, CC453_00825, CC652_10795, CC971_08240, CCP17_05605, CDZ72_00830, CE70_05375, CED07_09525, CEQ70_11810, CFF58_15570, CFF59_19345, CHN22_12865, CIX60_11710, CPS79_04635, CQO33_02710, CSG22_15805, CVR97_16715, D4361_00880, D4387_02960, D4422_08010, D5823_11460, D5N86_07595, D5N95_00880, D5O82_10090, D5P17_09705, D5X47_03750, D5Y28_11875, D6422_07755, D6J79_03215, D8S24_00875, DLB93_00880, DLR28_04665, DMI89_02725, DMO92_11765, DN165_06005, DNB97_10970, DNM27_08060, DNZ37_00880, DO533_01630, DP680_00880, DPB42_14145, DPD91_14800, DPF41_20265, DPF68_06590, DPS76_07075, DQD22_06090, DQR44_03755, DRM14_09660, DRT38_10160, DRT61_12455, DRV05_07095, DSF94_03190, DSG41_05900, DTF68_09765, DU071_11260, DU223_00815, DU657_08780, DU879_06935, DUV75_05405, DWU22_19580, DY580_11365, DYM27_07925, E0935_00855, E1A11_05885, E6W45_05500, EBD14_00860, EBK21_06270, EC404_08070, EEQ30_12970, EER35_04045, EHB09_10535, EL822_04920, ELS01_06375, EPB30_06160, EQG93_08445, EVY71_04545, EW905_00790, F0D96_17675, F2P00_07215, F3Q97_08560, F3R12_09955, F9G02_14055, F9O44_13860, FE758_17770, FEM52_12105, FGZ46_08045, FJM64_04970, FQC24_05140, G0038_06515, G0040_06835, G0042_12205, G0045_10420, G0047_05575, G0048_09090, G0051_05645, G0052_10845, G0059_05920, G0061_08170, G0062_08755, G0063_08645, G0067_09250, G0069_08825, G0070_07845, G0071_07185, G0072_20045, G0074_10225, G0076_07480, G0077_09020, G0080_10875, G0084_11635, G0086_11145, G0087_09090, G0088_08175, G0089_05200, G0090_03750, G0094_03980, G0100_14430, G0101_08275, G0102_07975, G0111_07605, G0113_09075, G0117_05810, G0123_09090, G0124_08815, G0148_07850, G0157_08505, G0170_09825, G0A05_14075, G0A28_05465, G0A32_06825, G0A39_18140, G0A43_17520, G0A44_05550, G0A46_06310, G0A50_04840, G0A51_06360, G0A52_16360, G0A53_03430, G0A56_14045, G0A58_04545, G0A60_05590, G0A61_07875, G0A63_17195, G0A66_04925, G0A67_08445, G0A68_18030, G0A70_04840, G0A73_06565, G0A76_16870, G0A79_08455, G0A92_06600, G0A96_06095, G0A97_04965, G0B03_06365, G0B05_02820, G0B07_02725, G0B08_03810, G0B12_00880, G0B28_10240, G0B96_06335, G0C03_03190, G0C04_07190, G0C34_08565, G0E15_12925, G0E20_13295, G0G84_14085, G0J24_05055, G0J26_08460, G0J27_00095, G0J28_06230, G0J31_03640, G0J33_04725, G0J34_09505, G0J36_06530, G0J37_09245, G0J40_08070, G0J43_05190, G0J44_09360, G0J45_06495, G0J46_07005, G0J47_09125, G0J49_07265, G0J50_09965, G0J51_08105, G0J53_03235, G0J55_05945, G0J58_04230, G0J59_20595, G0J62_02515, G0J65_11725, G0J66_02900, G0J67_06095, G0J69_03030, G0J71_03030, G0J73_12265, G0J76_03040, G0J79_08505, G0J81_05720, G0J82_07345, G0J85_05720, G0J89_12195, G0J92_11440, G0J94_12605, G0J96_11010, G0J97_05440, G0K00_10905, G0K02_08285, G0K03_06860, G0K04_04250, G0K05_00240, G0K07_03030, G0K10_07455, G0K13_06135, G0K15_04670, G0K16_11380, G0K18_09305, G0K19_03650, G0K20_01175, G0K23_06850, G0K25_00860, G0K26_09485, G0K28_12265, G0K30_06250, G0K31_02845, G0K32_21510, G0K33_05630, G0K37_00860, G0K38_10400, G0K39_00335, G0K41_00085, G0K42_08445, G0K44_09515, G0K46_05720, G0K47_10440, G0K48_11600, G0K49_12390, G0K52_13585, G0K53_13740, G0K56_12050, G0K58_17230, G0K59_13125, G0K61_08780, G0K65_07070, G0K68_05005, G0K70_08325, G0K72_03975, G0K74_16835, G0K75_06960, G0K78_11500, G0K80_19145, G0K83_05585, G0K84_13785, G0K85_10750, G0K88_003091, G0K89_000478, G0K90_000620, G0K94_000430, G0K95_000292, G0L00_000826, G0L02_000147, G0L03_04555, G0L06_01490, G0L07_01390, G0L10_04810, G0L14_03745, G0L15_14085, G0L18_01845, G0L19_04630, G0L20_03085, G0L24_03300, G0L25_01965, G0L29_02895, G0L31_11005, G0L32_05770, G0L34_00205, G0L35_02995, G0L36_06695, G0L37_03740, G0L38_01885, G0L40_13160, G0L42_04645, G0L48_07890, G0L49_00765, G0L51_03265, G0L52_00205, G0L55_04440, G0L59_01970, G0L62_04765, G0L63_03260, G0L65_18350, G0L67_02995, G0L68_05120, G0L70_10470, G0L73_04820, G0L76_03925, G0L77_17435, G0L78_01720, G0L79_04450, G0L83_11890, G0L85_15975, G0L86_000812, G0L88_02390, G0L89_04205, G0L91_04730, G0L93_17950, G0L96_03385, G0L98_18590, G0M00_19130, G0M05_16255, G0M06_000797, G0M13_000386, G0M14_02960, G0M16_02050, G0M18_000979, G0M21_11905, G0M22_000387, G0M25_000386, G0M26_03060, G0M29_000386, G0M30_05720, G0M33_12160, G0M35_05655, G0M36_14210, G0M38_01790, G0M39_00205, G0M40_05845, G0M41_07030, G0M45_06700, G0M46_000387, G0M48_000386, G0M51_18885, G0M53_03230, G0M55_04405, G0M56_01960, G0M58_01960, G0M63_03225, G0M65_01595, G0M67_04405, G0N45_04990, G0N48_08870, G0N51_12275, G0N53_04100, G0N55_07635, G0N57_04235, G0N58_04670, G0N59_10615, G0N60_09690, G0N61_05575, G0N62_05150, G0N64_07260, G0N65_06200, G0N66_09410, G0N67_07280, G0N71_08275, G0N75_10630, G0N78_06745, G0N82_06845, G0N84_07815, G0N85_04100, G0N86_11270, G0N88_07695, G0N89_06295, G0N90_07775, G0N92_16350, G0N94_02910, G0N95_11325, G0N98_10685, G0N99_03385, G0O00_03935, G0O03_07580, G0O10_06805, G0O14_02520, G0O15_13895, G0O18_14755, G0O19_09410, G0O20_06675, G0O22_05735, G0O25_08960, G0O27_07635, G0O31_08180, G0O32_05755, G0O36_06595, G0O37_08385, G0O39_05570, G0O40_13490, G0O41_08050, G0O42_08170, G0O43_09115, G0O44_05530, G0O47_08630, G0O52_07975, G0O55_07350, G0O57_07915, G0O58_10050, G0O59_14250, G0O60_09400, G0O63_04985, G0O66_13570, G0O68_07395, G0O70_08825, G0O71_08830, G0O74_07800, G0O75_07515, G0O77_08670, G0O78_08440, G0O80_07565, G0O81_03470, G0O82_10280, G0O84_08825, G0O85_05775, G0O86_10380, G0O87_07970, G0O88_03850, G0O89_06015, G0O92_08935, G0O93_04240, G0O94_13365, G0O97_02240, G0O99_13400, G0P00_15000, G0P01_02695, G0P02_06645, G0P05_12915, G0P06_08355, G0P08_09050, G0P12_13305, G0P13_05680, G0P17_06760, G0P18_07640, G0P19_05475, G0P24_03400, G0P26_08830, G0P28_08900, G0P30_08560, G0P31_10875, G0P36_12775, G0P37_02110, G0P41_15205, G0P44_03655, G0P45_07105, G0P48_07965, G0P49_00335, G0P52_05645, G0P53_00330, G0P56_10010, G0P57_08830, G0P58_04990, G0P63_06775, G0P65_04880, G0P67_10125, G0P68_07255, G0P69_00325, G0P73_13720, G0P75_01825, G0P76_03245, G1N61_05970, G1N64_05885, G1N66_05875, G1N68_05880, G1N71_05895, G1N72_05885, G1N86_05895, G1N87_05895, G1N91_06610, G1O00_03055, G1O02_05890, G1O04_05895, G1O05_05885, G1O08_05875, G1O10_07975, G1O12_05895, G1O16_05890, G1O17_05965, G1O18_05890, G1O20_05895, G1O23_05895, G1O25_05875, G1O26_05895, G1O27_05955, G1O28_05970, G1O29_05955, G1O32_05875, G1O34_05965, G1O38_06300, G1O40_05895, G1O43_05880, G1O46_03050, G1O48_05970, G1O49_05965, G1O51_05890, G1O53_05950, G1O62_05890, G1O63_05880, G1O65_05970, G1O67_07430, G1O68_05895, G1O69_05895, G1O71_09050, G1O72_05970, G1O76_05970, G1O77_05895, G1O80_05965, G1O81_05890, G1O83_05960, G1O84_05970, G1O87_05970, G1O88_05970, G1O89_05895, G1O90_05970, G1O93_05960, G1O94_05955, G1O96_05965, G1P02_05955, G1P03_05995, G1P06_05950, G1P09_03685, G1P10_05895, G1P12_05970, G1P14_00860, G1P15_08090, G1P17_05970, G1P19_05965, G1P23_05965, G1P24_05885, G1P25_05970, G1P26_05890, G1P29_05975, G1P31_05950, G1P35_05895, G1P36_05890, G1P37_05920, G1P40_03125, G1P44_05890, G1P45_05960, G1P47_05965, G1P48_05970, G1P51_05970, G1P52_05920, G1P53_05920, G1P54_05970, G1P55_05965, G1P56_05950, G1P57_05970, G1P58_05955, G1P59_05970, G1P61_08375, G1P64_05970, G1P67_05965, G1P69_05875, G1P72_10265, G1P75_05895, G1P76_05880, G1P78_03700, G1P83_03715, G1P84_05890, G1P87_05960, G1P90_05715, G1P91_05955, G1Q03_05890, G1Q08_05185, G1Q67_05960, G1Q78_05595, G1Q81_05965, G1Q83_05970, G1Q84_05625, G1Q85_05925, G1Q86_05880, G1Q88_03055, G1Q90_05955, G1Q91_05895, G1Q93_04950, G1Q96_05860, G1Q98_05895, G1Q99_05955, G1R01_05875, G1R02_03055, G1R03_05965, G1R04_05970, G1R08_05945, G1R13_05450, G1R15_05045, G1R20_03845, G1R21_05650, G1R22_03130, G1R23_03685, G1R27_05870, G1R28_05970, G1R29_05965, G1R30_05970, G1R31_06165, G1R36_05970, G1R38_05890, G1R40_03130, G1R42_03055, G1R44_05965, G1R45_07650, G1R47_03700, G1R48_03395, G1R51_05880, G1R52_08035, G1R53_05950, G1R63_05970, G1R69_00860, G1R87_03685, G1R93_05880, G1S02_05970, G2203_08010, G2212_16670, G2218_10055, G2221_14440, G2279_12050, G2290_12650, G2793_13330, G2918_13520, G2951_06210, G3221_002687, G3230_003613, G3231_002106, G3247_003536, G3248_001297, G3254_000041, G3263_002896, G3270_003435, G3275_000730, G3312_001759, G3336_003551, G3357_001662, G3369_000598, G3433_000039, G3460_000171, G3464_002693, G3593_003627, G3A35_06005, G3V06_000996, G3V14_003115, G3V17_000446, G3V21_001922, G3V56_000559, G3V57_001798, G3X03_001804, G4189_001061, G4190_000739, G4192_000812, G4198_000464, G4201_002101, G4202_001475, G4A01_003277, G4A73_001924, G4A83_004288, G4A85_000794, G4A87_001128, G4B68_002273, G4B72_002691, G4B74_000681, G4C74_000731, G4D32_002863, G4D46_002458, G4F88_00970, G4F89_00970, G4F91_00970, G4F92_00970, G4G47_002693, G4G62_000410, G4G67_002618, G4G68_003383, G4G75_001677, G4G76_001466, G4G79_002670, G4G97_002413, G4H00_000793, G4H04_004572, G4H07_003669, G4H08_000532, G4H18_000731, G4H21_000731, G4H24_000730, G4H63_001252, G4I66_003375, G4J07_001211, G4J08_002078, G4J11_000370, G4J12_000193, G4J18_002023, G4J20_003006, G4J37_004094, G4J39_002351, G4J41_001527, G4J45_002735, G4J90_001712, G4K02_000287, G4K03_000466, G4O54_000644, G4O56_001570, G4O59_000812, G4O60_000681, G4O67_000089, G4O69_001284, G4P29_001084, G4P83_001851, G4P85_000793, G4P89_003493, G4P91_001061, G4P93_001762, G4Q12_003651, G4Q28_000730, G4Q31_000728, G4Q50_002142, G4Q52_001801, G4Q59_002806, G4Q60_000793, G4Q63_002240, G4Q67_002883, G4Q94_003297, G4R01_000792, G4R02_002284, G4R15_002694, G4R16_000737, G4W68_003700, G4W73_000555, G4W86_000984, G4W87_000730, G4W88_000730, G4W91_000171, G4Y10_003993, G9269_000834, G9302_000793, G9304_001305, G9305_003574, G9309_000405, G9313_000793, G9314_004791, G9367_001474, G9381_000651, G9C24_000382, G9C41_000996, G9C46_000850, G9C47_000681, G9C49_001054, G9C57_000728, G9C64_002283, G9G03_000497, G9G04_003111, G9G34_001926, G9G36_000577, G9G45_002412, G9G50_002044, G9G62_002044, G9W19_001373, G9W28_001671, G9W45_000065, G9W52_002689, G9W63_002268, G9W65_000728, G9W79_002140, G9W95_000731, G9W96_002672, G9X40_002429, GB021_12045, GB040_11900, GB055_08450, GB076_09505, GB106_05935, GB114_08425, GB120_05560, GB122_13965, GB131_01480, GB139_06875, GB171_04135, GB209_06840, GB221_07870, GB224_00855, GB238_15785, GB280_05645, GB321_07520, GB331_07235, GB339_13210, GB342_05095, GB368_12215, GB372_06145, GB416_14990, GB452_06040, GB459_14220, GB466_12015, GB505_11895, GB510_10150, GB551_04205, GB567_10895, GB645_16180, GBS44_03925, GBS58_06025, GBV53_06495, GBV54_10940, GBV60_02935, GBW03_15445, GBW44_14930, GBW52_04530, GBW76_13765, GBX12_15340, GBX20_14950, GBX46_08290, GBX55_07645, GBX64_03960, GBY13_11225, GBY23_15345, GBY73_06075, GBZ51_19885, GBZ55_00575, GCZ80_15175, GEZ01_02980, GJE27_05655, GJE28_04530, GNA88_002718, GNA97_002865, GNA99_002432, GNB28_002419, GNB36_000624, GNB86_001092, GNC11_000727, GNC19_001070, GNC45_001165, GNC75_002133, GNC95_001305, GT380_05455, GTH60_03850, GTH62_02950, GTH63_08330, GTH66_08435, GTH67_11860, GTH68_08760, GTH70_03975, GTH72_08690, GTH73_05575, GTH75_06305, GTH77_02135, GTH78_08775, GTH79_06410, GTH81_13490, GTH85_09070, GTH87_09400, GTH89_05040, GTH90_06140, GTH91_00195, GTH93_08825, GTH94_05825, GTH99_03645, GXC51_05525, GXC56_05525, GXG40_05525, GYI58_17275, GYI62_000736, GYI77_09250, GYJ04_02110, GYJ24_05825, GYJ27_19115, GYJ28_003979, GYJ30_10035, GYJ32_11340, GYJ53_05965, GYJ59_05485, GYJ60_05955, H8S97_18675, JJB80_18360, JJB81_18355, KP44_05620, NG06_06920, R035_07945, Z700_03565, ZV17_16945, ZV33_03590, ZX03_04645, ZY40_05085 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: A0A2J0RDY6 #2: 化合物 | ChemComp-MG / | #3: 水 | ChemComp-HOH / | 研究の焦点であるリガンドがあるか | N | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.8 Å3/Da / 溶媒含有率: 56.03 % |
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7 詳細: 1% Tryptone 1mM Sodium azide 50mM HEPES pH 7.0 12% PEG3350 |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: シンクロトロン / サイト: Diamond / ビームライン: I03 / 波長: 0.976 Å |
検出器 | タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL / 日付: 2017年3月24日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.976 Å / 相対比: 1 |
反射 | 解像度: 1.94→34.5 Å / Num. obs: 9080 / % possible obs: 99.3 % / 冗長度: 5 % / Biso Wilson estimate: 46.12 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.049 / Rpim(I) all: 0.024 / Rrim(I) all: 0.055 / Net I/σ(I): 14.3 |
反射 シェル | 解像度: 1.94→1.97 Å / 冗長度: 5.2 % / Rmerge(I) obs: 1.076 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 475 / CC1/2: 0.655 / Rpim(I) all: 0.52 / % possible all: 99.2 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 7AK7 解像度: 1.95→34.49 Å / SU ML: 0.3 / 交差検証法: THROUGHOUT / σ(F): 1.99 / 位相誤差: 36.19 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
原子変位パラメータ | Biso max: 111.28 Å2 / Biso mean: 56.4175 Å2 / Biso min: 32.3 Å2 | ||||||||||||||||||||||||||||
精密化ステップ | サイクル: final / 解像度: 1.95→34.49 Å
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LS精密化 シェル | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 3
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