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- PDB-7z26: Crystal structure of YTHDF2 YTH domain in complex with m6A RNA -

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Basic information

Entry
Database: PDB / ID: 7z26
TitleCrystal structure of YTHDF2 YTH domain in complex with m6A RNA
Components
  • RNA (5'-R(P*(6MZ)P*CP*U)-3')
  • YTH domain-containing family protein 2
KeywordsRNA BINDING PROTEIN / epitranscriptomic reader / m6A reader
Function / homology
Function and homology information


C5-methylcytidine-containing RNA reader activity / organelle assembly / spermatogonial cell division / regulation of meiotic cell cycle process involved in oocyte maturation / positive regulation of cap-independent translational initiation / regulation of rRNA processing / gamete generation / endothelial to hematopoietic transition / embryonic morphogenesis / N6-methyladenosine-containing RNA reader activity ...C5-methylcytidine-containing RNA reader activity / organelle assembly / spermatogonial cell division / regulation of meiotic cell cycle process involved in oocyte maturation / positive regulation of cap-independent translational initiation / regulation of rRNA processing / gamete generation / endothelial to hematopoietic transition / embryonic morphogenesis / N6-methyladenosine-containing RNA reader activity / negative regulation of stem cell differentiation / oocyte maturation / regulation of hematopoietic stem cell differentiation / negative regulation of type I interferon-mediated signaling pathway / hematopoietic stem cell proliferation / mRNA destabilization / mRNA catabolic process / humoral immune response / negative regulation of Notch signaling pathway / regulation of neurogenesis / regulation of cell adhesion / regulation of mRNA stability / stress granule assembly / P-body / centriolar satellite / cytoplasmic ribonucleoprotein granule / cytoplasmic stress granule / innate immune response / mRNA binding / RNA binding / nucleus / cytoplasm / cytosol
Similarity search - Function
YTH domain containing protein / YTH domain / YT521-B-like domain / YTH domain profile.
Similarity search - Domain/homology
RNA / YTH domain-containing family protein 2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å
AuthorsNai, F. / Nachawati, R. / Li, Y. / Caflisch, A.
Funding support Switzerland, 1items
OrganizationGrant numberCountry
Swiss National Science Foundation310030B-189363 Switzerland
CitationJournal: Acs Med.Chem.Lett. / Year: 2022
Title: Fragment Ligands of the m 6 A-RNA Reader YTHDF2.
Authors: Nai, F. / Nachawati, R. / Zalesak, F. / Wang, X. / Li, Y. / Caflisch, A.
History
DepositionFeb 25, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 16, 2022Provider: repository / Type: Initial release
Revision 1.1Sep 28, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Jun 14, 2023Group: Derived calculations / Category: pdbx_struct_assembly / pdbx_struct_assembly_gen
Revision 1.3Feb 7, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: YTH domain-containing family protein 2
B: YTH domain-containing family protein 2
D: RNA (5'-R(P*(6MZ)P*CP*U)-3')
E: RNA (5'-R(P*(6MZ)P*CP*U)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)42,2207
Polymers41,9364
Non-polymers2843
Water3,153175
1
A: YTH domain-containing family protein 2
D: RNA (5'-R(P*(6MZ)P*CP*U)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)21,0643
Polymers20,9682
Non-polymers961
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: YTH domain-containing family protein 2
E: RNA (5'-R(P*(6MZ)P*CP*U)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)21,1564
Polymers20,9682
Non-polymers1882
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)80.690, 80.690, 114.270
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number170
Space group name H-MP65
Space group name HallP65
Symmetry operation#1: x,y,z
#2: x-y,x,z+5/6
#3: y,-x+y,z+1/6
#4: -y,x-y,z+2/3
#5: -x+y,-x,z+1/3
#6: -x,-y,z+1/2

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Components

#1: Protein YTH domain-containing family protein 2 / CLL-associated antigen KW-14 / High-glucose-regulated protein 8 / Renal carcinoma antigen NY-REN-2


Mass: 19367.848 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: YTHDF2, HGRG8 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9Y5A9
#2: RNA chain RNA (5'-R(P*(6MZ)P*CP*U)-3')


Mass: 1600.035 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#3: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#4: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 175 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.91 Å3/Da / Density % sol: 57.7 %
Crystal growTemperature: 277.15 K / Method: vapor diffusion, hanging drop
Details: 2.025 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.2

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Aug 28, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.9→40.34 Å / Num. obs: 33384 / % possible obs: 99.9 % / Redundancy: 9.06 % / Biso Wilson estimate: 28.61 Å2 / CC1/2: 0.999 / Net I/σ(I): 20.97
Reflection shellResolution: 1.9→2.01 Å / Num. unique obs: 5365 / CC1/2: 0.878

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Processing

Software
NameVersionClassification
PHENIX1.20_4459refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4RDN

4rdn


Resolution: 1.9→40.34 Å / SU ML: 0.2171 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 23.9331
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2302 1667 5 %
Rwork0.1973 31649 -
obs0.1989 33316 99.89 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 31.72 Å2
Refinement stepCycle: LAST / Resolution: 1.9→40.34 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2346 127 16 176 2665
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00832605
X-RAY DIFFRACTIONf_angle_d1.0583553
X-RAY DIFFRACTIONf_chiral_restr0.0635375
X-RAY DIFFRACTIONf_plane_restr0.0074436
X-RAY DIFFRACTIONf_dihedral_angle_d12.4218389
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.9-1.950.32451380.27422608X-RAY DIFFRACTION99.28
1.95-2.020.28361380.26012634X-RAY DIFFRACTION99.93
2.02-2.090.27191400.23592640X-RAY DIFFRACTION100
2.09-2.170.26361370.21952614X-RAY DIFFRACTION99.89
2.17-2.270.22021380.21112641X-RAY DIFFRACTION99.93
2.27-2.390.2681380.2032625X-RAY DIFFRACTION99.93
2.39-2.540.24631390.20612646X-RAY DIFFRACTION99.96
2.54-2.740.24281370.20842613X-RAY DIFFRACTION99.96
2.74-3.010.24131400.20552649X-RAY DIFFRACTION100
3.01-3.450.22561390.18562644X-RAY DIFFRACTION100
3.45-4.340.18521410.16792657X-RAY DIFFRACTION100
4.34-40.340.22371420.18932678X-RAY DIFFRACTION99.89

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