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- PDB-7yoi: Crystal structure of I88L single mutant of O-acetylserine sulfhyd... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7yoi | ||||||
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Title | Crystal structure of I88L single mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high-affinity inhibitory peptide from serine acetyltransferase of Salmonella typhimurium at 2.14 A | ||||||
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![]() | TRANSFERASE/TRANSFERASE INHIBITOR / Transferase / single mutant / O-acetyl-L-serine sulfhydrylase / Haemophilus influenzae / TRANSFERASE-TRANSFERASE INHIBITOR complex | ||||||
Function / homology | ![]() cysteine synthase / cystathionine beta-synthase activity / cysteine synthase activity / L-cysteine desulfhydrase activity / cysteine biosynthetic process from serine / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kumar, N. / Rahisuddin, R. / Saini, N. / Singh, A.K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of I88L single mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high-affinity inhibitory peptide from serine acetyltransferase of Salmonella ...Title: Crystal structure of I88L single mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high-affinity inhibitory peptide from serine acetyltransferase of Salmonella typhimurium at 2.14 A Authors: Kumar, N. / Rahisuddin, R. / Saini, N. / Singh, A.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.8 KB | Display | ![]() |
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PDB format | ![]() | 52.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.1 KB | Display | ![]() |
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Full document | ![]() | 444 KB | Display | |
Data in XML | ![]() | 13.7 KB | Display | |
Data in CIF | ![]() | 18.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7yohC ![]() 7yomC ![]() 1y7lS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33651.465 Da / Num. of mol.: 1 / Mutation: I88L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: cysK, HI_1103 / Production host: ![]() ![]() |
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#2: Protein/peptide | Mass: 900.930 Da / Num. of mol.: 1 / Source method: obtained synthetically Source: (synth.) ![]() |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.8 % |
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Crystal grow | Temperature: 278 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.1M HEPES, 1.3M Sodium Citrate / PH range: 7.3-7.9 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Sep 10, 2012 |
Radiation | Monochromator: Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54179 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→41.454 Å / Num. obs: 15711 / % possible obs: 97.64 % / Redundancy: 14.6 % / CC1/2: 0.873 / CC star: 0.965 / Net I/σ(I): 73.27 |
Reflection shell | Resolution: 2.14→2.217 Å / Num. unique obs: 1218 / CC1/2: 0.77 / % possible all: 76.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1Y7L Resolution: 2.14→41.454 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.902 / SU B: 8.74 / SU ML: 0.226 / Cross valid method: FREE R-VALUE / ESU R: 0.324 / ESU R Free: 0.239 Details: Hydrogens have been used if present in the input file
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.896 Å2
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Refinement step | Cycle: LAST / Resolution: 2.14→41.454 Å
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Refine LS restraints |
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LS refinement shell |
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