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- PDB-7yjj: Human Cytosolic 10-formyltetrahydrofolate dehydrogenase and Gossy... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7yjj | ||||||
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Title | Human Cytosolic 10-formyltetrahydrofolate dehydrogenase and Gossypol complex | ||||||
![]() | Cytosolic 10-formyltetrahydrofolate dehydrogenase | ||||||
![]() | OXIDOREDUCTASE / Cytosolic 10-formyltetrahydrofolate dehydrogenase / Aldehyde dehydrogenase family 1 member L1 / inhibitor / Gossypol | ||||||
Function / homology | ![]() formyltetrahydrofolate dehydrogenase / formyltetrahydrofolate dehydrogenase activity / 10-formyltetrahydrofolate catabolic process / NADPH regeneration / Metabolism of folate and pterines / aldehyde dehydrogenase (NAD+) activity / biosynthetic process / one-carbon metabolic process / extracellular exosome / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 6.31 Å | ||||||
![]() | Han, C.W. / Lee, H.N. / Jeong, M.S. / Jang, S.B. | ||||||
Funding support | 1items
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![]() | ![]() Title: Human Cytosolic 10-formyltetrahydrofolate dehydrogenase and Gossypol complex Authors: Han, C.W. / Lee, H.N. / Jeong, M.S. / Jang, S.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 344.1 KB | Display | ![]() |
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PDB format | ![]() | 274.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 93.8 KB | Display | |
Data in CIF | ![]() | 138.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 33872MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 54439.246 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: O75891, formyltetrahydrofolate dehydrogenase #2: Chemical | ChemComp-GO3 / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Human Cytosolic 10-formyltetrahydrofolate dehydrogenase and Gossypol complex Type: COMPLEX Details: Protein (Human Cytosolic 10-formyltetrahydrofolate dehydrogenase) and chemical (Gossypol) complex Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 0.223 MDa / Experimental value: YES |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: NITROGEN |
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Electron microscopy imaging
Microscopy | Model: TFS GLACIOS |
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Electron gun | Electron source: ![]() |
Electron lens | Mode: DARK FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Average exposure time: 54.56 sec. / Electron dose: 39.55 e/Å2 / Film or detector model: FEI FALCON III (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 6.31 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 396292 / Symmetry type: POINT | ||||||||||||||||||||||||
Refinement | Stereochemistry target values: CDL v1.2 | ||||||||||||||||||||||||
Refine LS restraints |
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