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- PDB-7ya2: Crystal structure of capsular polysaccharide synthesis enzyme Cap... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7ya2 | ||||||
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Title | Crystal structure of capsular polysaccharide synthesis enzyme CapG from Staphylococcus aureus | ||||||
![]() | Capsular polysaccharide synthesis enzyme CapG | ||||||
![]() | ISOMERASE / 2-epimerase | ||||||
Function / homology | UDP-N-acetylglucosamine 2-epimerase WecB-like / UDP-N-acetylglucosamine 2-epimerase domain / UDP-N-acetylglucosamine 2-epimerase / UDP-N-acetylglucosamine 2-epimerase activity / Capsular polysaccharide synthesis enzyme CapG![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chen, Y. / Wang, Y.C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of the capsular polysaccharide-synthesis enzyme CapG from Staphylococcus aureus. Authors: Tien, N. / Ho, C.Y. / Lai, S.J. / Lin, Y.C. / Yang, C.S. / Wang, Y.C. / Huang, W.C. / Chen, Y. / Chang, J.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 444.3 KB | Display | ![]() |
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PDB format | ![]() | 363.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 484.4 KB | Display | ![]() |
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Full document | ![]() | 563 KB | Display | |
Data in XML | ![]() | 80.2 KB | Display | |
Data in CIF | ![]() | 106.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4hwgS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 42905.785 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.48 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.2 M ammonium citrate tribasic pH 7.2, 18 % w/v polyethylene glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 13, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→30 Å / Num. obs: 56178 / % possible obs: 99.4 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.126 / Net I/σ(I): 9.675 |
Reflection shell | Resolution: 3.2→3.31 Å / Redundancy: 3.4 % / Num. unique obs: 5591 / CC1/2: 0.591 / CC star: 0.862 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4HWG Resolution: 3.2→25.57 Å / Cross valid method: THROUGHOUT Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 74.66 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2→25.57 Å
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Refine LS restraints |
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