+Open data
-Basic information
Entry | Database: PDB / ID: 7xwy | ||||||
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Title | Crystal structure of spFft3 N-terminal truncation | ||||||
Components | ATP-dependent helicase fft3 | ||||||
Keywords | DNA BINDING PROTEIN / DNA binding / remodeler / nucleosome / Fft3 | ||||||
Function / homology | Function and homology information attachment of telomeric heterochromatin to nuclear envelope / ATP-dependent H3-H4 histone complex chaperone activity / histone chaperone activity / transcription elongation-coupled chromatin remodeling / replication fork processing / ATP-dependent activity, acting on DNA / heterochromatin / helicase activity / DNA helicase / damaged DNA binding ...attachment of telomeric heterochromatin to nuclear envelope / ATP-dependent H3-H4 histone complex chaperone activity / histone chaperone activity / transcription elongation-coupled chromatin remodeling / replication fork processing / ATP-dependent activity, acting on DNA / heterochromatin / helicase activity / DNA helicase / damaged DNA binding / chromatin remodeling / chromatin binding / chromatin / ATP hydrolysis activity / DNA binding / ATP binding / nucleus Similarity search - Function | ||||||
Biological species | Schizosaccharomyces pombe 972h- (yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.25 Å | ||||||
Authors | Zhang, N. / Jiang, T. / Huo, Y. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Crystal structure of spFft3 N-terminal truncation Authors: Zhang, N. / Jiang, T. / Huo, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7xwy.cif.gz | 95.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7xwy.ent.gz | 70.4 KB | Display | PDB format |
PDBx/mmJSON format | 7xwy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xw/7xwy ftp://data.pdbj.org/pub/pdb/validation_reports/xw/7xwy | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 45042.922 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Schizosaccharomyces pombe 972h- (yeast) Strain: 972 / ATCC 24843 / Gene: fft3, snf2SR, SPAC25A8.01c / Production host: Escherichia coli (E. coli) / References: UniProt: O42861, DNA helicase |
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#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.89 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop Details: 0.2 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 30% w/v Polyethylene glycol monomethyl ether 5,000 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.97 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 13, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→38.132 Å / Num. obs: 33411 / % possible obs: 99.02 % / Redundancy: 6.6 % / CC1/2: 0.971 / Net I/σ(I): 9.2 |
Reflection shell | Resolution: 2.25→2.31 Å / Num. unique obs: 1220 / CC1/2: 0.905 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.25→38.132 Å / SU ML: 0.25 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 24.46 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 74.87 Å2 / Biso mean: 30.0479 Å2 / Biso min: 9.45 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.25→38.132 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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