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Open data
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Basic information
Entry | Database: PDB / ID: 7xmw | ||||||
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Title | Crystal structure of anti-CRISPR protein AcrVIA2 | ||||||
![]() | AcrVIA2 | ||||||
![]() | ANTIVIRAL PROTEIN / a bacteria antiviral protein | ||||||
Function / homology | SELENIUM ATOM / Uncharacterized protein![]() | ||||||
Biological species | Leptotrichia wadei | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yan, X. / Li, X. / Song, G. | ||||||
Funding support | 1items
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![]() | ![]() Title: Structure of AcrVIA2 and its binding mechanism to CRISPR-Cas13a. Authors: Song, G. / Li, X. / Wang, Z. / Dong, C. / Xie, X. / Yan, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.3 KB | Display | ![]() |
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PDB format | ![]() | 74.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 780.7 KB | Display | ![]() |
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Full document | ![]() | 784.7 KB | Display | |
Data in XML | ![]() | 8.5 KB | Display | |
Data in CIF | ![]() | 10.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 8352.190 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: F0279 / Gene: HMPREF9015_01064 / Production host: ![]() ![]() #2: Chemical | #3: Chemical | #4: Chemical | ChemComp-SE / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.76 Å3/Da / Density % sol: 74.17 % |
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Crystal grow | Temperature: 291.15 K / Method: liquid diffusion / Details: TRIS hydrochloride, ammonium phosphate monobasic |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 24, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 2.59→41.13 Å / Num. obs: 9880 / % possible obs: 92.89 % / Redundancy: 2 % / Biso Wilson estimate: 84.03 Å2 / CC1/2: 0.999 / Net I/σ(I): 51.14 |
Reflection shell | Resolution: 2.59→2.683 Å / Num. unique obs: 535 / CC1/2: 0.733 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 95.43 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.59→41.13 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 32.6340708388 Å / Origin y: 35.9876254129 Å / Origin z: 11.8229401988 Å
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Refinement TLS group | Selection details: all |