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- PDB-7xlb: Cryo-EM structure of human pannexin 2 -

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Basic information

Entry
Database: PDB / ID: 7xlb
TitleCryo-EM structure of human pannexin 2
ComponentsPannexin-2
KeywordsMEMBRANE PROTEIN / ATP channel / Calcium channel / heptamer
Function / homology
Function and homology information


Electric Transmission Across Gap Junctions / wide pore channel activity / positive regulation of interleukin-1 production / gap junction channel activity / monoatomic cation transport / response to ischemia / cell-cell signaling / monoatomic ion transmembrane transport / Golgi membrane / endoplasmic reticulum membrane ...Electric Transmission Across Gap Junctions / wide pore channel activity / positive regulation of interleukin-1 production / gap junction channel activity / monoatomic cation transport / response to ischemia / cell-cell signaling / monoatomic ion transmembrane transport / Golgi membrane / endoplasmic reticulum membrane / protein-containing complex binding / structural molecule activity / plasma membrane
Similarity search - Function
Pannexin / Innexin / Innexin / Pannexin family profile.
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.44 Å
AuthorsHang, Z. / Huawei, Z. / Daping, W.
Funding support China, 3items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31900046 China
National Natural Science Foundation of China (NSFC)81972085 China
National Natural Science Foundation of China (NSFC)82172465 China
CitationJournal: Nat Commun / Year: 2023
Title: Cryo-EM structure of human heptameric pannexin 2 channel
Authors: Zhang, H. / Wang, S. / Zhang, Z. / Hou, M. / Du, C. / Zhao, Z. / Vogel, H. / Li, Z. / Yan, K. / Zhang, X. / Lu, J. / Liang, Y. / Yuan, S. / Wang, D. / Zhang, H.
History
DepositionApr 21, 2022Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Mar 15, 2023Provider: repository / Type: Initial release
Revision 1.1Nov 6, 2024Group: Data collection / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / em_admin / pdbx_entry_details / pdbx_modification_feature
Item: _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
E: Pannexin-2
A: Pannexin-2
B: Pannexin-2
C: Pannexin-2
D: Pannexin-2
F: Pannexin-2
G: Pannexin-2


Theoretical massNumber of molelcules
Total (without water)324,1117
Polymers324,1117
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein
Pannexin-2


Mass: 46301.543 Da / Num. of mol.: 7
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PANX2 / Cell line (production host): 293F / Production host: Homo sapiens (human) / References: UniProt: Q96RD6
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Heptamer of human pannexin 2 channel / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightValue: 0.323 MDa / Experimental value: NO
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human) / Cell: 293F
Buffer solutionpH: 7.6
Buffer component
IDConc.NameFormulaBuffer-ID
150 mM2-Amino-2-hydroxymethyl-1,3-propanediolTris1
2150 mMsodium chlorideNaCl1
30.02 %Glyco-diosgeninGDN1
SpecimenConc.: 10 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)
Image scansMovie frames/image: 32

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Processing

Software
NameVersionClassificationNB
phenix.real_space_refine1.20.1_4487refinement
PHENIX1.20.1_4487refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.44 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 108265 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 17.88 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.003319530
ELECTRON MICROSCOPYf_angle_d0.613526432
ELECTRON MICROSCOPYf_chiral_restr0.03843045
ELECTRON MICROSCOPYf_plane_restr0.01063325
ELECTRON MICROSCOPYf_dihedral_angle_d5.15142569

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