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Open data
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Basic information
Entry | Database: PDB / ID: 7x1i | |||||||||
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Title | Cryo-EM structure of human BTR1 in the outward-facing state. | |||||||||
![]() | Isoform 1 of Solute carrier family 4 member 11 | |||||||||
![]() | MEMBRANE PROTEIN / SLC4A11 / BTR1 / SLC transporter | |||||||||
Function / homology | ![]() borate transport / active borate transmembrane transporter activity / regulation of mesenchymal stem cell differentiation / fluid transport / water transmembrane transporter activity / monoatomic ion homeostasis / intracellular monoatomic cation homeostasis / cellular hypotonic response / proton channel activity / solute:inorganic anion antiporter activity ...borate transport / active borate transmembrane transporter activity / regulation of mesenchymal stem cell differentiation / fluid transport / water transmembrane transporter activity / monoatomic ion homeostasis / intracellular monoatomic cation homeostasis / cellular hypotonic response / proton channel activity / solute:inorganic anion antiporter activity / symporter activity / bicarbonate transport / bicarbonate transmembrane transporter activity / monoatomic anion transport / vesicle membrane / sodium channel activity / sodium ion transport / proton transmembrane transporter activity / transmembrane transporter activity / proton transmembrane transport / regulation of mitochondrial membrane potential / transmembrane transport / cellular response to oxidative stress / basolateral plasma membrane / protein dimerization activity / apical plasma membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.94 Å | |||||||||
![]() | Yin, Y. / Lu, Y. / Zuo, P. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into the conformational changes of BTR1/SLC4A11 in complex with PIP. Authors: Yishuo Lu / Peng Zuo / Hongyi Chen / Hui Shan / Weize Wang / Zonglin Dai / He Xu / Yayu Chen / Ling Liang / Dian Ding / Yan Jin / Yuxin Yin / ![]() Abstract: BTR1 (SLC4A11) is a NH stimulated H (OH) transporter belonging to the SLC4 family. Dysfunction of BTR1 leads to diseases such as congenital hereditary endothelial dystrophy (CHED) and Fuchs ...BTR1 (SLC4A11) is a NH stimulated H (OH) transporter belonging to the SLC4 family. Dysfunction of BTR1 leads to diseases such as congenital hereditary endothelial dystrophy (CHED) and Fuchs endothelial corneal dystrophy (FECD). However, the mechanistic basis of BTR1 activation by alkaline pH, transport activity regulation and pathogenic mutations remains elusive. Here, we present cryo-EM structures of human BTR1 in the outward-facing state in complex with its activating ligands PIP and the inward-facing state with the pathogenic R125H mutation. We reveal that PIP binds at the interface between the transmembrane domain and the N-terminal cytosolic domain of BTR1. Disruption of either the PIP binding site or protonation of PIP phosphate groups by acidic pH can transform BTR1 into an inward-facing conformation. Our results provide insights into the mechanisms of how the transport activity and conformation changes of BTR1 are regulated by PIP binding and interaction of TMD and NTD. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 252.9 KB | Display | ![]() |
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PDB format | ![]() | 199.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 32942MC ![]() 7x1gC ![]() 7x1hC ![]() 7x1jC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 99684.438 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Solute carrier family 4 member 11, isoform B / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 0.199 MDa / Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
EM software | Name: cryoSPARC / Version: 3.1.0 / Category: 3D reconstruction |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3D reconstruction | Resolution: 2.94 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 92740 / Symmetry type: POINT |