+Open data
-Basic information
Entry | Database: PDB / ID: 7wzb | ||||||||||||
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Title | lipopolysaccharide assembly protein LapB (open) | ||||||||||||
Components | Lipopolysaccharide assembly protein B | ||||||||||||
Keywords | MEMBRANE PROTEIN / lipopolysaccharide assembly protein LapB (open) cytoplasmic soluble domain | ||||||||||||
Function / homology | Function and homology information lipopolysaccharide metabolic process / regulation of lipid biosynthetic process / transferase activity / iron ion binding / cell division / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||||||||
Authors | Yan, L. / Dong, H. / Li, H. / Liu, X. / Deng, Z. / Dong, C. / Zhang, Z. | ||||||||||||
Funding support | China, 3items
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Citation | Journal: To Be Published Title: lipopolysaccharide assembly protein LapB (open) Authors: Yan, L. / Dong, H. / Li, H. / Liu, X. / Deng, Z. / Dong, C. / Zhang, Z. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7wzb.cif.gz | 212.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7wzb.ent.gz | 165.9 KB | Display | PDB format |
PDBx/mmJSON format | 7wzb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7wzb_validation.pdf.gz | 598.7 KB | Display | wwPDB validaton report |
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Full document | 7wzb_full_validation.pdf.gz | 602 KB | Display | |
Data in XML | 7wzb_validation.xml.gz | 19.1 KB | Display | |
Data in CIF | 7wzb_validation.cif.gz | 25.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wz/7wzb ftp://data.pdbj.org/pub/pdb/validation_reports/wz/7wzb | HTTPS FTP |
-Related structure data
Related structure data | 4zlhS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 42383.277 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: lipopolysaccharide assembly protein B Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria) Strain: LT2 / Gene: lapB / Plasmid: pet28 / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q7CQG6 #2: Chemical | #3: Chemical | ChemComp-PGE / | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.92 % |
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Crystal grow | Temperature: 293 K / Method: evaporation / pH: 8 / Details: 21.25% Ethylene glycol 15% glycerol / PH range: 7-8 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9175 Å |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Apr 27, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9175 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→24.72 Å / Num. obs: 19835 / % possible obs: 97.03 % / Redundancy: 7.3 % / Biso Wilson estimate: 41.78 Å2 / CC1/2: 1 / CC star: 1 / Rmerge(I) obs: 0.04174 / Rpim(I) all: 0.01674 / Rrim(I) all: 0.04506 / Net I/σ(I): 27.93 |
Reflection shell | Resolution: 2.7→2.796 Å / Redundancy: 7.08 % / Rmerge(I) obs: 0.2306 / Mean I/σ(I) obs: 7.08 / Num. unique obs: 1537 / CC1/2: 0.991 / CC star: 0.998 / Rpim(I) all: 0.08943 / Rrim(I) all: 0.2476 / % possible all: 78.14 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4ZLH Resolution: 2.7→24.46 Å / Cross valid method: THROUGHOUT / Stereochemistry target values: ML
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Solvent computation | Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 148.98 Å2 / Biso mean: 73.9629 Å2 / Biso min: 37.84 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.7→24.46 Å
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LS refinement shell | Resolution: 2.7→2.796 Å / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Origin x: 13.8983 Å / Origin y: -17.2672 Å / Origin z: -9.0542 Å
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Refinement TLS group |
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