+Open data
-Basic information
Entry | Database: PDB / ID: 7wsp | ||||||
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Title | Structure of a membrane protein M | ||||||
Components |
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Keywords | IMMUNE SYSTEM / membrane | ||||||
Function / homology | Function and homology information hexameric IgM immunoglobulin complex / IgM B cell receptor complex / pentameric IgM immunoglobulin complex / B cell receptor complex / pre-B cell allelic exclusion / IgM immunoglobulin complex / CD22 mediated BCR regulation / B cell proliferation / B cell activation / immunoglobulin complex, circulating ...hexameric IgM immunoglobulin complex / IgM B cell receptor complex / pentameric IgM immunoglobulin complex / B cell receptor complex / pre-B cell allelic exclusion / IgM immunoglobulin complex / CD22 mediated BCR regulation / B cell proliferation / B cell activation / immunoglobulin complex, circulating / complement activation, classical pathway / multivesicular body / B cell differentiation / antigen binding / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Cell surface interactions at the vascular wall / B cell receptor signaling pathway / transmembrane signaling receptor activity / antibacterial humoral response / defense response to Gram-negative bacterium / adaptive immune response / blood microparticle / Potential therapeutics for SARS / immune response / membrane raft / external side of plasma membrane / innate immune response / cell surface / signal transduction / extracellular space / extracellular exosome / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.09 Å | ||||||
Authors | Ma, X. / Zhu, Y. / Chen, Y. / Huang, Z. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Structure of a membrane protein M Authors: Ma, X. / Zhu, Y. / Chen, Y. / Huang, Z. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7wsp.cif.gz | 185.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7wsp.ent.gz | 146.8 KB | Display | PDB format |
PDBx/mmJSON format | 7wsp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7wsp_validation.pdf.gz | 788.9 KB | Display | wwPDB validaton report |
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Full document | 7wsp_full_validation.pdf.gz | 800.1 KB | Display | |
Data in XML | 7wsp_validation.xml.gz | 32.8 KB | Display | |
Data in CIF | 7wsp_validation.cif.gz | 49.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ws/7wsp ftp://data.pdbj.org/pub/pdb/validation_reports/ws/7wsp | HTTPS FTP |
-Related structure data
Related structure data | 32763MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 15504.718 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CD79A, IGA, MB1 / Production host: Homo sapiens (human) / References: UniProt: P11912 |
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#2: Protein | Mass: 16143.744 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CD79B, B29, IGB / Production host: Homo sapiens (human) / References: UniProt: P40259 |
#3: Protein | Mass: 40470.355 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IGHM / Production host: Homo sapiens (human) / References: UniProt: P01871 |
#4: Protein | Mass: 40599.535 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IGHM / Production host: Homo sapiens (human) / References: UniProt: P01871 |
Has protein modification | Y |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: complex / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: NITROGEN |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 1.5 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 4.09 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 47428 / Symmetry type: POINT |