+Open data
-Basic information
Entry | Database: PDB / ID: 7wkf | ||||||
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Title | Antimicrobial peptide-LaIT2 | ||||||
Components | Beta-KTx-like peptide LaIT2 | ||||||
Keywords | STRUCTURAL PROTEIN / CYANA 2.1 Scorpion Antimicrobial peptide | ||||||
Function / homology | Long chain scorpion toxin family / Potassium channel toxin / BetaSPN-type cysteine-stabilized alpha/beta (CS-alpha/beta) domain profile. / toxin activity / defense response to bacterium / extracellular region / Beta-KTx-like peptide LaIT2 Function and homology information | ||||||
Biological species | Liocheles australasiae (scorpion) | ||||||
Method | SOLUTION NMR / simulaed annealing | ||||||
Authors | Tamura, M. / Morita, H. / Ohki, S. | ||||||
Funding support | 1items
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Citation | Journal: Toxicon / Year: 2022 Title: Structural and functional studies of LaIT2, an antimicrobial and insecticidal peptide from Liocheles australasiae. Authors: Tamura, M. / Tatsushiro, C. / Morita, E.H. / Ohki, S. #1: Journal: Biosci. Biotechnol. Biochem / Year: 2009 Title: Purification and cDNA cloning of LaIT2, a novel insecticidal toxin from venom of the scorpion Liocheles australasiae Authors: Matsushita, M.N. / Miyashita, M.M. / Ichiki, I.Y. / Ogura, O.T. / Sakuradani, S.E. / Nakagawa, N.Y. / Shimizu, S.S. / Miyagawa, M.H. #2: Journal: J. Pept. Sci / Year: 2018 Title: Chemical synthesis of a two-domain scorpion toxin LaIT2 and its single-domain analogs to elucidate structural factors important for insecticidal and antimicrobial activities Authors: Juichi, H.J. / Ando, A.R. / Ishido, I.T. / Miyashita, M.M. / Ogura, O.T. / Sakuradani, S.E. / Nakagawa, N.Y. / Miyagawa, M.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7wkf.cif.gz | 415.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7wkf.ent.gz | 361.4 KB | Display | PDB format |
PDBx/mmJSON format | 7wkf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7wkf_validation.pdf.gz | 404.5 KB | Display | wwPDB validaton report |
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Full document | 7wkf_full_validation.pdf.gz | 503.2 KB | Display | |
Data in XML | 7wkf_validation.xml.gz | 18.2 KB | Display | |
Data in CIF | 7wkf_validation.cif.gz | 28.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wk/7wkf ftp://data.pdbj.org/pub/pdb/validation_reports/wk/7wkf | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6650.886 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Liocheles australasiae (scorpion) / Plasmid: pET-mod / Production host: Escherichia coli (E. coli) / References: UniProt: C7G3K3 |
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Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 mM / Label: LaIT2 / pH: 6.0 / Pressure: 1.013 Pa / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: simulaed annealing / Software ordinal: 5 | ||||||||||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |