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Open data
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Basic information
Entry | Database: PDB / ID: 7w8h | ||||||
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Title | Sweet taste protein Brazzein mutant - D29K | ||||||
![]() | (Defensin-like protein) x 2 | ||||||
![]() | PLANT PROTEIN / Sweet taste protein / artficial sweetener | ||||||
Function / homology | Knottin, scorpion toxin-like superfamily / defense response to fungus / killing of cells of another organism / defense response to bacterium / extracellular region / Defensin-like protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, T. / Yoon, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Sweet taste protein Brazzein mutant - D29K Authors: Kim, T. / Yoon, T. #2: Journal: FEBS Lett / Year: 1994 Title: Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. Authors: Ming, D. / Hellekant, G. #1: Journal: FEBS Lett / Year: 2003 Title: Critical regions for the sweetness of brazzein. Authors: Jin, Z. / Danilova, V. / Assadi-Porter, F.M. / Aceti, D.J. / Markley, J.L. / Hellekant, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127.8 KB | Display | ![]() |
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PDB format | ![]() | 81.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4heqS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 6525.517 Da / Num. of mol.: 7 / Mutation: D29K Source method: isolated from a genetically manipulated source Details: Sweet tasting Protein Brazzein mutant D29K / Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | | Mass: 6511.425 Da / Num. of mol.: 1 / Mutation: D29K Source method: isolated from a genetically manipulated source Details: Sweet tasting Protein Brazzein mutant D29K / Source: (gene. exp.) ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.61 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 4.5 / Details: 0-1.5M NaCl 1M Na-Acetate pH4.5 / PH range: 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Jul 22, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 1.501→27.32 Å / Num. obs: 87242 / % possible obs: 99.72 % / Redundancy: 9.8 % / Biso Wilson estimate: 21.0709049292 Å2 / CC1/2: 0.996 / CC star: 0.999 / Net I/σ(I): 17.34 |
Reflection shell | Resolution: 1.501→3.26 Å / Num. unique obs: 8676 / CC1/2: 0.996 / CC star: 0.999 / Rpim(I) all: 0.094 / Rrim(I) all: 0.302 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4heq Resolution: 1.50100444366→27.3145642247 Å / SU ML: 0.199638708669 / Cross valid method: FREE R-VALUE / σ(F): 1.96224378477 / Phase error: 29.5769340776
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.4584594636 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.50100444366→27.3145642247 Å
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Refine LS restraints |
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LS refinement shell |
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