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Open data
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Basic information
Entry | Database: PDB / ID: 7w68 | ||||||
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Title | human single hexameric Mcm2-7 complex | ||||||
![]() | (DNA replication licensing factor ...) x 6 | ||||||
![]() | REPLICATION / complex | ||||||
Function / homology | ![]() Switching of origins to a post-replicative state / Unwinding of DNA / nuclear origin of replication recognition complex / alpha DNA polymerase:primase complex / mitotic DNA replication / CMG complex / single-stranded 3'-5' DNA helicase activity / regulation of phosphorylation / MCM complex / mitotic DNA replication initiation ...Switching of origins to a post-replicative state / Unwinding of DNA / nuclear origin of replication recognition complex / alpha DNA polymerase:primase complex / mitotic DNA replication / CMG complex / single-stranded 3'-5' DNA helicase activity / regulation of phosphorylation / MCM complex / mitotic DNA replication initiation / double-strand break repair via break-induced replication / regulation of DNA-templated DNA replication initiation / single-stranded DNA helicase activity / DNA strand elongation involved in DNA replication / cochlea development / DNA unwinding involved in DNA replication / 3'-5' DNA helicase activity / DNA replication origin binding / Activation of the pre-replicative complex / DNA replication initiation / cellular response to interleukin-4 / Activation of ATR in response to replication stress / Assembly of the pre-replicative complex / Orc1 removal from chromatin / nucleosome assembly / cellular response to xenobiotic stimulus / single-stranded DNA binding / histone binding / DNA helicase / DNA replication / cell population proliferation / chromosome, telomeric region / cell cycle / centrosome / apoptotic process / DNA damage response / chromatin / perinuclear region of cytoplasm / enzyme binding / ATP hydrolysis activity / DNA binding / nucleoplasm / ATP binding / identical protein binding / membrane / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.4 Å | ||||||
![]() | Xu, N.N. / Lin, Q.P. / Liu, C.D. / Tian, H.L. / Xiang, Y. / Zhu, G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: human single hexameric Mcm2-7 complex Authors: Zhu, G. / Xiang, Y. / Xu, N.N. / Lin, Q.P. / Liu, C.D. / Tian, H.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 614.3 KB | Display | ![]() |
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PDB format | ![]() | 474 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 840.9 KB | Display | ![]() |
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Full document | ![]() | 980.5 KB | Display | |
Data in XML | ![]() | 110 KB | Display | |
Data in CIF | ![]() | 161.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 32326MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
-DNA replication licensing factor ... , 6 types, 6 molecules ABCDEF
#1: Protein | Mass: 102034.102 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Protein | Mass: 91110.852 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#3: Protein | Mass: 96684.852 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#4: Protein | Mass: 82406.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#5: Protein | Mass: 93010.273 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#6: Protein | Mass: 81411.875 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: CELL / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: minichromosome maintenance 2-7 / Type: COMPLEX / Entity ID: all / Source: NATURAL |
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Molecular weight | Value: 570 kDa/nm / Experimental value: YES |
Source (natural) | Organism: ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: NITROGEN / Humidity: 100 % / Chamber temperature: 8 K |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: DIFFRACTION / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
Software | Name: PHENIX / Version: dev_3311: / Classification: refinement |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3D reconstruction | Resolution: 4.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 186918 / Symmetry type: POINT |