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- PDB-7vw7: Crystal structure of the 2 ADP-AlF4-bound V1 complex -

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Basic information

Entry
Database: PDB / ID: 7vw7
TitleCrystal structure of the 2 ADP-AlF4-bound V1 complex
Components
  • (V-type sodium ATPase subunit ...) x 3
  • V-type sodium ATPase catalytic subunit A
KeywordsMOTOR PROTEIN / P-loop / Hydrolase / Na(+)-ATPase / ATP binding
Function / homology
Function and homology information


proton-transporting ATP synthase complex / proton motive force-driven plasma membrane ATP synthesis / H+-transporting two-sector ATPase / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / hydrolase activity / ATP binding
Similarity search - Function
ATPase, V1 complex, subunit F, bacterial/archaeal / ATPase, V1 complex, subunit D / ATPase, V1 complex, subunit F / ATPase, V1 complex, subunit F superfamily / ATP synthase subunit D / ATP synthase (F/14-kDa) subunit / V-type ATP synthase regulatory subunit B/beta / V-type ATP synthase catalytic alpha chain / ATPsynthase alpha/beta subunit, N-terminal extension / ATPsynthase alpha/beta subunit N-term extension ...ATPase, V1 complex, subunit F, bacterial/archaeal / ATPase, V1 complex, subunit D / ATPase, V1 complex, subunit F / ATPase, V1 complex, subunit F superfamily / ATP synthase subunit D / ATP synthase (F/14-kDa) subunit / V-type ATP synthase regulatory subunit B/beta / V-type ATP synthase catalytic alpha chain / ATPsynthase alpha/beta subunit, N-terminal extension / ATPsynthase alpha/beta subunit N-term extension / ATPase, F1/V1 complex, beta/alpha subunit, C-terminal / ATP synthase subunit alpha, N-terminal domain-like superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain / ATP synthase alpha/beta family, beta-barrel domain / ATPase, alpha/beta subunit, nucleotide-binding domain, active site / ATP synthase alpha and beta subunits signature. / ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain / ATP synthase alpha/beta family, nucleotide-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
ADENOSINE-5'-DIPHOSPHATE / TETRAFLUOROALUMINATE ION / V-type ATP synthase alpha chain / V-type ATP synthase subunit F / V-type ATP synthase beta chain / V-type ATP synthase subunit D
Similarity search - Component
Biological speciesEnterococcus hirae (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.818 Å
AuthorsSuzuki, K. / Shekhar, M. / Gupta, C. / Singharoy, A. / Murata, T.
Funding support Japan, 1items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS) Japan
CitationJournal: Acs Cent.Sci. / Year: 2022
Title: Revealing a Hidden Intermediate of Rotatory Catalysis with X-ray Crystallography and Molecular Simulations.
Authors: Shekhar, M. / Gupta, C. / Suzuki, K. / Chan, C.K. / Murata, T. / Singharoy, A.
History
DepositionNov 9, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 22, 2022Provider: repository / Type: Initial release
Revision 1.1Aug 17, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: V-type sodium ATPase catalytic subunit A
B: V-type sodium ATPase catalytic subunit A
C: V-type sodium ATPase catalytic subunit A
D: V-type sodium ATPase subunit B
E: V-type sodium ATPase subunit B
F: V-type sodium ATPase subunit B
G: V-type sodium ATPase subunit D
H: V-type sodium ATPase subunit NtpG (F)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)399,26515
Polymers398,0648
Non-polymers1,2017
Water181
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area36150 Å2
ΔGint-158 kcal/mol
Surface area121400 Å2
MethodPISA
Unit cell
Length a, b, c (Å)127.740, 128.870, 231.570
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 1 types, 3 molecules ABC

#1: Protein V-type sodium ATPase catalytic subunit A / V-ATPase subunit A


Mass: 67473.273 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria)
Gene: ntpA, atpA, BHG15_05195, E5348_07375, EB10_01559, GUI85_04285, NCTC12204_02059
Production host: Escherichia coli (E. coli) / Strain (production host): ntpA
References: UniProt: A0A1V8WY35, H+-transporting two-sector ATPase

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V-type sodium ATPase subunit ... , 3 types, 5 molecules DEFGH

#2: Protein V-type sodium ATPase subunit B / V-ATPase subunit B


Mass: 52424.312 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria)
Gene: ntpB, atpB, BHG15_05190, E5348_07370, EB10_01558, NCTC12204_02058
Production host: Escherichia coli (E. coli) / References: UniProt: A0A1V8XC32
#3: Protein V-type sodium ATPase subunit D


Mass: 25587.996 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria) / Gene: ntpD, I6I80_06820, NCTC12204_02057 / Production host: Escherichia coli (E. coli)
References: UniProt: A0A7Z9AX30, H+-transporting two-sector ATPase
#4: Protein V-type sodium ATPase subunit NtpG (F) / V-ATPase subunit F


Mass: 12783.129 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria)
Gene: ntpG, atpF, BHG15_05200, E5348_07380, EB10_01560, GUI85_04280, NCTC12204_02060
Production host: Escherichia coli (E. coli) / References: UniProt: A0A1V8XC17

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Non-polymers , 5 types, 8 molecules

#5: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / Adenosine diphosphate


Mass: 427.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: ADP, energy-carrying molecule*YM
#7: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#8: Chemical ChemComp-ALF / TETRAFLUOROALUMINATE ION


Mass: 102.975 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: AlF4 / Feature type: SUBJECT OF INVESTIGATION
#9: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.39 Å3/Da / Density % sol: 48.63 %
Crystal growTemperature: 296 K / Method: vapor diffusion, sitting drop / pH: 6.5 / Details: Bis Tris propane, NaF, PEG-3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 0.98 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 20, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.98 Å / Relative weight: 1
ReflectionResolution: 3.818→46.128 Å / Num. obs: 71426 / % possible obs: 99.5 % / Redundancy: 3.904 % / Biso Wilson estimate: 119.54 Å2 / CC1/2: 0.993 / Rmerge(I) obs: 0.172 / Rrim(I) all: 0.199 / Χ2: 0.908 / Net I/σ(I): 7.61
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
3.818-4.053.8810.9531.544443511565114490.5921.10599
4.05-4.333.9210.5562.624244710864108250.8330.64399.6
4.33-4.673.940.3084.483974310117100870.9380.35799.7
4.67-5.113.9340.2495.5436528930892850.9570.28899.8
5.11-5.713.9270.255.5432821837983580.9520.2999.7
5.71-6.573.9240.1897.1729192745474400.970.21999.8
6.57-8.023.890.0913.4424582633863190.9930.10599.7
8.02-11.193.8510.04126.418740487448660.9980.04799.8
11.19-46.1283.7060.03631.4310367285627970.9980.04297.9

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation9.28 Å46.13 Å
Translation9.28 Å46.13 Å

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Processing

Software
NameVersionClassification
XSCALEdata scaling
PHASER2.5.6phasing
PHENIX1.12-2829refinement
PDB_EXTRACT3.27data extraction
XDSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3VR5

3vr5


Resolution: 3.818→46.128 Å / SU ML: 0.55 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 29.06 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2671 3559 4.98 %
Rwork0.226 67847 -
obs0.2281 71406 99.56 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 309.41 Å2 / Biso mean: 148.6814 Å2 / Biso min: 67.79 Å2
Refinement stepCycle: final / Resolution: 3.818→46.128 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms24999 0 72 1 25072
Biso mean--118.58 135.53 -
Num. residues----3381
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00325492
X-RAY DIFFRACTIONf_angle_d0.50934668
X-RAY DIFFRACTIONf_chiral_restr0.0433981
X-RAY DIFFRACTIONf_plane_restr0.0044591
X-RAY DIFFRACTIONf_dihedral_angle_d12.69615341
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.818-3.87010.41491430.3529271098
3.8701-3.92540.37851410.3442266999
3.9254-3.98390.35381430.33082720100
3.9839-4.04610.43831450.3223269099
4.0461-4.11240.36011410.2992728100
4.1124-4.18330.33251410.2961269299
4.1833-4.25930.30411470.25722762100
4.2593-4.34120.25241430.2405267499
4.3412-4.42970.26861440.22122755100
4.4297-4.5260.22281480.2162684100
4.526-4.63110.24861390.21492705100
4.6311-4.74690.29251430.21152760100
4.7469-4.87510.3151440.21552686100
4.8751-5.01830.25411430.22532709100
5.0183-5.18010.2731470.24342744100
5.1801-5.3650.29291390.2512699100
5.365-5.57950.2821400.24052716100
5.5795-5.83290.27721450.23392732100
5.8329-6.13980.32061390.24292732100
6.1398-6.52350.26211390.22322711100
6.5235-7.02550.26591410.2172708100
7.0255-7.72960.24091380.21052722100
7.7296-8.84120.2051420.17012718100
8.8412-11.11320.17551400.1452723100
11.1132-46.1280.23191440.2121269899
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
1-0.04910.04960.04260.04350.08540.031-0.06290.17360.7239-0.0606-0.94230.1126-0.11530.3962-01.37120.4484-0.00461.4930.11121.1427-60.005810.6087-1.7732
20.09880.1416-0.12520.1386-0.08550.045-1.5467-0.2872-0.0397-0.38150.4469-0.5665-0.2393-1.0122-01.28880.2649-0.00221.3926-0.10721.7016-59.272213.44032.8062
30.016-0.08650.0370.0480.0292-0.05940.28280.2766-0.064-0.48340.03370.7144-0.50920.069101.50070.2596-0.11540.90910.01011.2113-46.789327.8368-0.1074
40.271-0.08490.2351-0.27560.08830.09260.36510.2611-0.1025-0.0048-0.452-0.0555-0.713-0.0823-01.88980.36370.00071.53240.27251.4688-41.478635.5459-8.4989
5-0.0698-0.12890.07890.07590.28310.20260.62330.80820.30910.43-0.62330.47-0.12710.0026-01.53120.2214-0.06311.49720.29310.7165-39.890728.5398-14.5323
60.5819-0.08960.55290.3013-0.21420.407-0.1153-0.06590.56730.0929-0.15040.093-0.32890.0279-01.41050.06740.20180.85540.02381.3077-32.007328.223410.841
70.3043-0.530.01890.20650.08250.16620.23670.0966-0.2973-0.1251-0.2991-0.2543-0.20670.1978-01.28180.01720.11770.87370.07161.0028-31.646815.48444.3428
80.32830.06420.20370.203-0.05690.03730.2276-0.1476-0.46570.227-0.2478-1.0542-0.2535-0.4967-01.3085-0.05770.15650.8589-0.01521.2277-17.995521.576217.4319
90.06440.0149-0.33920.09980.33780.8850.281-0.32730.33260.0177-0.42260.3331-0.09390.497501.6126-0.44080.15251.3139-0.07881.5164-2.536633.799426.8447
100.216-0.00670.40770.92340.5616-1.60050.23870.4409-1.21540.11990.2175-0.5101-1.0251-0.6314-00.6354-0.1597-0.26740.7511-0.39912.1288-31.2387-38.02577.8271
110.3086-0.194-0.27480.31070.1350.63570.0034-0.1752-0.3740.0111-0.0147-0.1188-0.33560.041100.883-0.0359-0.36710.78150.16431.2086-15.3727-26.327936.662
120.15350.2061-0.12820.1041-0.18750.09740.1927-0.1864-0.7885-0.15290.0151-0.0754-0.1619-0.200201.26750.0001-0.1681.31310.2051.6525-3.1984-33.578534.9373
13-0.1310.24280.1381-0.0947-0.0350.04070.3775-0.0418-0.610.1714-0.6011-0.803-0.03190.508901.43660.0479-0.48171.38520.20261.97187.081-31.566541.9778
140.0857-0.0339-0.01460.0721-0.07010.14180.0789-0.5602-0.4786-0.3333-0.41110.3431-0.0291-0.3869-01.1334-0.1162-0.02851.8254-0.41951.0801-77.4992-16.604624.4736
150.8791-0.64250.74160.83980.42620.45040.0052-0.4389-0.17990.24-0.1750.35280.1475-0.09501.0724-0.21580.25982.2212-0.1580.7848-78.6384-6.503151.7716
160.015-0.1735-0.73741.5471-0.1360.11670.1635-0.41760.1232-0.2106-0.07550.12410.1442-0.3389-01.1161-0.13550.0411.77670.00660.927-64.3719-5.630146.0843
17-0.09730.30710.12580.1932-0.23930.29250.5693-1.5527-0.4216-0.41241.12335.02740.14680.0177-01.1406-0.19821.02262.498-3.041-7.6435-40.34964.228865.9473
180.071-0.0591-0.14190.0171-0.13930.0808-0.7799-0.28250.2566-0.2837-0.2715-0.3177-0.0171-0.605101.41190.24260.07071.5844-0.2241.4545-75.69925.341414.4054
190.22960.1558-0.02490.2783-0.24860.10760.33410.19990.7476-0.0728-0.06160.5903-0.3426-0.615101.26660.2330.13531.4841-0.00621.211-77.84456.769719.8007
200.0619-0.05450.04530.0135-0.0237-0.03910.9666-0.03240.473-0.7495-0.60230.811-0.1263-0.3779-01.51070.48350.3311.6666-0.36431.5553-73.536927.808832.2161
210.8211-0.10360.54030.0091-0.50510.68690.0028-0.28960.37070.0380.09840.0384-0.144-0.552-01.14610.15320.31111.246-0.38761.0625-56.292524.300432.0779
220.1613-0.46951.31450.0183-0.43080.30220.06060.36180.27830.0083-0.21460.192-0.1617-0.2234-01.1582-0.04020.16691.163-0.48291.1606-34.398926.730345.5485
230.0427-0.06810.00450.1801-0.04080.0196-0.56390.33-0.08280.35910.4435-0.07590.5908-0.22901.59290.18270.16011.4389-0.13210.9998-46.8209-3.1242-11.3256
24-0.0138-0.05940.0960.0381-0.0810.08950.0049-0.149-0.1797-0.1678-0.1799-0.31710.0167-0.048501.33180.28090.09631.31980.04691.1997-45.246-2.1989-7.9762
250.79240.00250.09321.1651-0.11990.3251-0.00360.5734-0.3391-0.0961-0.1257-0.2476-0.34650.144700.88720.03160.1771.0321-0.33420.9007-14.8751-7.086-4.1032
26-0.00510.0485-0.1192-0.00660.0492-0.0355-0.30870.9392-0.0055-0.4217-0.03140.0431-0.66710.274601.18420.05240.31551.3697-0.34591.5404-14.61216.2453-4.335
270.66040.0765-0.3995-0.24040.42150.73630.08320.0112-0.2074-0.06370.186-0.1648-0.0515-0.1171-00.92950.04730.06880.7174-0.14940.9863-18.1994-8.73136.7742
280.29310.14940.22590.3834-0.00540.14610.05250.00780.19650.25680.2288-0.5666-0.17430.035701.05760.0876-0.09081.2649-0.39041.84896.9388-8.055421.9243
290.23130.0086-0.04540.097-0.03230.10340.2009-0.9231-0.1155-0.7841-0.0826-0.55340.3624-0.381501.4891-0.2204-0.16971.2475-0.1341.9997-63.3579-34.674313.1038
300.06880.1303-0.0299-0.11940.35650.26350.50350.645-0.98870.3615-0.7809-0.2009-0.2262-0.234701.1643-0.1292-0.03271.18-0.21081.2696-61.573-33.450215.276
310.5068-0.03260.3472-0.03410.03910.53230.2651-0.7298-1.2484-0.0096-0.3745-0.39680.215-0.278201.1271-0.2815-0.16441.35780.77211.6581-48.7288-38.476943.2354
320.7923-0.0066-0.60570.38580.1760.2083-0.3046-0.421-0.47-0.1866-0.08430.0164-0.4041-0.062800.9758-0.2077-0.12181.18620.43091.0098-49.3078-26.753538.3192
33-0.04770.07540.3341-0.1860.3316-0.1950.5085-1.6832-1.1136-0.7475-1.08412.10040.2121.4061-01.1989-0.4529-0.03572.50520.7097-0.2213-32.9286-17.272159.8254
34-0.098-0.03640.2602-0.06150.09530.0261.1264-1.2797-0.609-0.1334-0.5159-0.29450.271.1676-01.8425-0.2314-0.20143.15831.8108-2.8857-34.0767-23.267767.3294
35-0.1354-0.24260.54940.84220.8775-0.27140.5411.0347-0.21080.4997-0.1501-0.52530.1712-0.0752-01.4741-0.1543-0.55481.56160.05061.3124-8.28795.89150.8452
36-0.3083-0.53780.41640.4755-0.0197-0.15060.49441.39650.75431.0588-0.5559-0.6736-0.1145-1.078102.1975-0.6043-0.84930.9820.2131.88762.96683.503268.5108
370.07250.0864-0.12960.18470.25330.14460.3353-0.0484-0.2632-0.4150.0391-0.3743-0.0060.2525-01.2948-0.02740.19711.1870.09011.2157-37.586-4.885426.3765
38-0.05230.01-0.0353-0.0173-0.00270.07230.8343-0.1333-0.40411.06910.4853-0.3137-0.3644-0.344902.649-0.2159-0.69142.44170.50991.96749.0508-4.958881.3422
39-0.0064-0.00490.00360.006-0.00410.00010.10640.0784-0.1792-0.16910.1465-0.32870.1422-0.2087-03.941-0.2583-0.51452.46270.02982.32910.3441-13.351976.2396
400.0041-0.0152-0.0216-0.0354-0.0085-0.0299-0.06720.0486-0.0320.29640.4381-0.1321-0.2491-0.113903.3054-1.0289-0.5222.56950.5542.71212.0232-3.469176.2908
410.0268-0.00270.0177-0.0040.02940.08450.0088-0.221-0.39030.51120.6948-0.2624-0.5943-0.917202.234-0.3425-0.57721.8028-0.24242.14862.9969-7.764668.1482
42-0.008-0.0010.00920.0085-0.00640.02290.1915-0.26840.1112-0.06810.10190.1765-0.15390.1563-0.00012.40480.3786-0.35031.7443-0.02083.540118.5231-6.454267.811
430.01810.0104-0.00320.00030.0050.00330.3640.1417-0.25440.002-0.0551-0.1843-0.2352-0.1653-01.7064-0.1586-0.12882.1507-0.3932.59096.7467-0.204153.7688
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 0 through 26 )A0 - 26
2X-RAY DIFFRACTION2chain 'A' and (resid 27 through 63 )A27 - 63
3X-RAY DIFFRACTION3chain 'A' and (resid 64 through 90 )A64 - 90
4X-RAY DIFFRACTION4chain 'A' and (resid 91 through 162 )A91 - 162
5X-RAY DIFFRACTION5chain 'A' and (resid 163 through 202 )A163 - 202
6X-RAY DIFFRACTION6chain 'A' and (resid 203 through 344 )A203 - 344
7X-RAY DIFFRACTION7chain 'A' and (resid 345 through 390 )A345 - 390
8X-RAY DIFFRACTION8chain 'A' and (resid 391 through 452 )A391 - 452
9X-RAY DIFFRACTION9chain 'A' and (resid 453 through 587 )A453 - 587
10X-RAY DIFFRACTION10chain 'B' and (resid -6 through 390 )B-6 - 390
11X-RAY DIFFRACTION11chain 'B' and (resid 391 through 499 )B391 - 499
12X-RAY DIFFRACTION12chain 'B' and (resid 500 through 536 )B500 - 536
13X-RAY DIFFRACTION13chain 'B' and (resid 537 through 586 )B537 - 586
14X-RAY DIFFRACTION14chain 'C' and (resid 1 through 63 )C1 - 63
15X-RAY DIFFRACTION15chain 'C' and (resid 64 through 248 )C64 - 248
16X-RAY DIFFRACTION16chain 'C' and (resid 249 through 416 )C249 - 416
17X-RAY DIFFRACTION17chain 'C' and (resid 417 through 579 )C417 - 579
18X-RAY DIFFRACTION18chain 'D' and (resid 4 through 28 )D4 - 28
19X-RAY DIFFRACTION19chain 'D' and (resid 29 through 77 )D29 - 77
20X-RAY DIFFRACTION20chain 'D' and (resid 78 through 106 )D78 - 106
21X-RAY DIFFRACTION21chain 'D' and (resid 107 through 305 )D107 - 305
22X-RAY DIFFRACTION22chain 'D' and (resid 306 through 455 )D306 - 455
23X-RAY DIFFRACTION23chain 'E' and (resid 4 through 38 )E4 - 38
24X-RAY DIFFRACTION24chain 'E' and (resid 39 through 68 )E39 - 68
25X-RAY DIFFRACTION25chain 'E' and (resid 69 through 187 )E69 - 187
26X-RAY DIFFRACTION26chain 'E' and (resid 188 through 217 )E188 - 217
27X-RAY DIFFRACTION27chain 'E' and (resid 218 through 364 )E218 - 364
28X-RAY DIFFRACTION28chain 'E' and (resid 365 through 455 )E365 - 455
29X-RAY DIFFRACTION29chain 'F' and (resid 1 through 38 )F1 - 38
30X-RAY DIFFRACTION30chain 'F' and (resid 39 through 77 )F39 - 77
31X-RAY DIFFRACTION31chain 'F' and (resid 78 through 217 )F78 - 217
32X-RAY DIFFRACTION32chain 'F' and (resid 218 through 340 )F218 - 340
33X-RAY DIFFRACTION33chain 'F' and (resid 341 through 410 )F341 - 410
34X-RAY DIFFRACTION34chain 'F' and (resid 411 through 453 )F411 - 453
35X-RAY DIFFRACTION35chain 'G' and (resid 6 through 63 )G6 - 63
36X-RAY DIFFRACTION36chain 'G' and (resid 64 through 172 )G64 - 172
37X-RAY DIFFRACTION37chain 'G' and (resid 173 through 206 )G173 - 206
38X-RAY DIFFRACTION38chain 'H' and (resid 2 through 29 )H2 - 29
39X-RAY DIFFRACTION39chain 'H' and (resid 30 through 42 )H30 - 42
40X-RAY DIFFRACTION40chain 'H' and (resid 43 through 51 )H43 - 51
41X-RAY DIFFRACTION41chain 'H' and (resid 52 through 76 )H52 - 76
42X-RAY DIFFRACTION42chain 'H' and (resid 77 through 84 )H77 - 84
43X-RAY DIFFRACTION43chain 'H' and (resid 85 through 101 )H85 - 101

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