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Yorodumi- PDB-7vu7: The Solution structure of the C-terminal domain from flagelliform... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7vu7 | |||||||||
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Title | The Solution structure of the C-terminal domain from flagelliform spidroin | |||||||||
Components | Flagelliform fibroin | |||||||||
Keywords | STRUCTURAL PROTEIN / flagelliform spidroin / CTD | |||||||||
Function / homology | Spidroin, C-terminal domain superfamily / Flagelliform fibroin Function and homology information | |||||||||
Biological species | Araneus ventricosus (diadem spider) | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Authors | Fan, J.S. / Yang, D. | |||||||||
Funding support | Singapore, 2items
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Citation | Journal: Biomacromolecules / Year: 2022 Title: C-Terminal Domains of Spider Silk Proteins Having Divergent Structures but Conserved Functional Roles. Authors: Li, X. / Fan, J.S. / Shi, M. / Lai, C.C. / Li, J. / Meng, Q. / Yang, D. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7vu7.cif.gz | 993.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7vu7.ent.gz | 839 KB | Display | PDB format |
PDBx/mmJSON format | 7vu7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7vu7_validation.pdf.gz | 489.6 KB | Display | wwPDB validaton report |
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Full document | 7vu7_full_validation.pdf.gz | 965.7 KB | Display | |
Data in XML | 7vu7_validation.xml.gz | 96.3 KB | Display | |
Data in CIF | 7vu7_validation.cif.gz | 109.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vu/7vu7 ftp://data.pdbj.org/pub/pdb/validation_reports/vu/7vu7 | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11014.216 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Araneus ventricosus (diadem spider) / Production host: Escherichia coli (E. coli) / References: UniProt: A0FK72 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 1.5 mM [U-100% 13C; U-100% 15N] CTD, 95% H2O/5% D2O Label: CN_sample / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 1.5 mM / Component: CTD / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 150 mM / Ionic strength err: 0.2 / Label: con_1 / pH: 7 / PH err: 0.05 / Pressure: 1 atm / Pressure err: 0.01 / Temperature: 298 K / Temperature err: 0.1 |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz / Details: cryoprobe |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |