Japan Agency for Medical Research and Development (AMED)
JP20am0101329
Japan
Citation
Journal: Febs J. / Year: 2022 Title: Structure-based engineering of a short-chain cis-prenyltransferase to biosynthesize nonnatural all-cis-polyisoprenoids: molecular mechanisms for primer substrate recognition and ultimate ...Title: Structure-based engineering of a short-chain cis-prenyltransferase to biosynthesize nonnatural all-cis-polyisoprenoids: molecular mechanisms for primer substrate recognition and ultimate product chain-length determination. Authors: Kutsukawa, R. / Imaizumi, R. / Suenaga-Hiromori, M. / Takeshita, K. / Sakai, N. / Misawa, S. / Yamamoto, M. / Yamaguchi, H. / Miyagi-Inoue, Y. / Waki, T. / Kataoka, K. / Nakayama, T. / ...Authors: Kutsukawa, R. / Imaizumi, R. / Suenaga-Hiromori, M. / Takeshita, K. / Sakai, N. / Misawa, S. / Yamamoto, M. / Yamaguchi, H. / Miyagi-Inoue, Y. / Waki, T. / Kataoka, K. / Nakayama, T. / Yamashita, S. / Takahashi, S.
Resolution: 1.94→49.36 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.94 / SU B: 6.004 / SU ML: 0.159 / Cross valid method: THROUGHOUT / ESU R: 0.161 / ESU R Free: 0.159 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.25502
2260
5.3 %
RANDOM
Rwork
0.19999
-
-
-
obs
0.20298
40769
99.91 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK