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Yorodumi- PDB-7vma: The X-ray crystallographic structure of amylo-alpha-1,6-glucosida... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7vma | ||||||||||||
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Title | The X-ray crystallographic structure of amylo-alpha-1,6-glucosidase from Thermococcus gammatolerans STB12 | ||||||||||||
Components | Amylo-alpha-1,6-glucosidase, putative archaeal type glycogen debranching enzyme (Gde) | ||||||||||||
Keywords | HYDROLASE / Amylo-alpha-1 / 6-glucosidase | ||||||||||||
Function / homology | Function and homology information amylo-alpha-1,6-glucosidase / amylo-alpha-1,6-glucosidase activity / carbohydrate metabolic process Similarity search - Function | ||||||||||||
Biological species | Thermococcus gammatolerans EJ3 (archaea) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.804 Å | ||||||||||||
Authors | Li, Z.F. / Ban, X.F. / Wang, Y.M. / Li, C.M. / Gu, Z.B. | ||||||||||||
Funding support | China, 3items
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Citation | Journal: To Be Published Title: The X-ray Crystallographic Structure of Amylo-alpha-1,6-glucosidase from Thermococcus gannatilerans STB12 Authors: Li, Z.F. / Ban, X.F. / Wang, Y.M. / Li, C.M. / Gu, Z.B. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7vma.cif.gz | 245.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7vma.ent.gz | 197.8 KB | Display | PDB format |
PDBx/mmJSON format | 7vma.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7vma_validation.pdf.gz | 449.9 KB | Display | wwPDB validaton report |
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Full document | 7vma_full_validation.pdf.gz | 494.2 KB | Display | |
Data in XML | 7vma_validation.xml.gz | 48.3 KB | Display | |
Data in CIF | 7vma_validation.cif.gz | 65.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vm/7vma ftp://data.pdbj.org/pub/pdb/validation_reports/vm/7vma | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 68705.500 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermococcus gammatolerans EJ3 (archaea) Strain: DSM 15229 / JCM 11827 / EJ3 / Gene: gde, TGAM_1568 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: C5A758, amylo-alpha-1,6-glucosidase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.71 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / Details: 25%tertiary butanol; 0.1M Tris |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.9785 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 7, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 2.52→48.77 Å / Num. obs: 44230 / % possible obs: 99.8 % / Redundancy: 6.6 % / Rmerge(I) obs: 0.087 / Net I/σ(I): 12.3 |
Reflection shell | Resolution: 2.52→2.6 Å / Rmerge(I) obs: 0.022 / Num. unique obs: 4569 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: predict using alpha-fold Resolution: 2.804→38.998 Å / SU ML: 0.6 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 42.57 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 150.94 Å2 / Biso mean: 94.3893 Å2 / Biso min: 57.17 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.804→38.998 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 11
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