+Open data
-Basic information
Entry | Database: PDB / ID: 7vge | ||||||
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Title | Structure of the PDZ deleted variant of HtrA2 protease (S306A) | ||||||
Components | (Serine protease HTRA2, mitochondrial) x 3 | ||||||
Keywords | HYDROLASE / Serine protease / HtrA / PDZ / oligomer | ||||||
Function / homology | Function and homology information HtrA2 peptidase / pentacyclic triterpenoid metabolic process / negative regulation of mitophagy in response to mitochondrial depolarization / positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway / ceramide metabolic process / regulation of autophagy of mitochondrion / mitochondrial protein catabolic process / CD40 receptor complex / : / programmed cell death ...HtrA2 peptidase / pentacyclic triterpenoid metabolic process / negative regulation of mitophagy in response to mitochondrial depolarization / positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway / ceramide metabolic process / regulation of autophagy of mitochondrion / mitochondrial protein catabolic process / CD40 receptor complex / : / programmed cell death / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / serine-type endopeptidase complex / adult walking behavior / response to herbicide / positive regulation of protein targeting to mitochondrion / execution phase of apoptosis / positive regulation of execution phase of apoptosis / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / protein autoprocessing / negative regulation of cell cycle / regulation of multicellular organism growth / neuron development / cellular response to interferon-beta / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / cellular response to retinoic acid / forebrain development / Mitochondrial protein degradation / serine-type peptidase activity / mitochondrial membrane / protein catabolic process / mitochondrial intermembrane space / cytoplasmic side of plasma membrane / cellular response to growth factor stimulus / intrinsic apoptotic signaling pathway in response to DNA damage / unfolded protein binding / peptidase activity / cellular response to heat / cellular response to oxidative stress / neuron apoptotic process / negative regulation of neuron apoptotic process / cytoskeleton / positive regulation of apoptotic process / serine-type endopeptidase activity / chromatin / endoplasmic reticulum membrane / endoplasmic reticulum / mitochondrion / proteolysis / identical protein binding / membrane / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 4 Å | ||||||
Authors | Parui, A.L. / Mishra, V. / Bhaumik, P. / Bose, K. | ||||||
Funding support | India, 1items
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Citation | Journal: Structure / Year: 2022 Title: Inter-subunit crosstalk via PDZ synergistically governs allosteric activation of proapoptotic HtrA2. Authors: Parui, A.L. / Mishra, V. / Dutta, S. / Bhaumik, P. / Bose, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7vge.cif.gz | 419.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7vge.ent.gz | 352.4 KB | Display | PDB format |
PDBx/mmJSON format | 7vge.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7vge_validation.pdf.gz | 471.8 KB | Display | wwPDB validaton report |
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Full document | 7vge_full_validation.pdf.gz | 482.5 KB | Display | |
Data in XML | 7vge_validation.xml.gz | 36.7 KB | Display | |
Data in CIF | 7vge_validation.cif.gz | 50.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vg/7vge ftp://data.pdbj.org/pub/pdb/validation_reports/vg/7vge | HTTPS FTP |
-Related structure data
Related structure data | 1lcyS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 21668.580 Da / Num. of mol.: 3 / Mutation: S306A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HTRA2, OMI, PRSS25 / Production host: Escherichia coli (E. coli) / References: UniProt: O43464, HtrA2 peptidase #2: Protein | Mass: 21555.422 Da / Num. of mol.: 2 / Mutation: S306A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HTRA2, OMI, PRSS25 / Production host: Escherichia coli (E. coli) / References: UniProt: O43464, HtrA2 peptidase #3: Protein | | Mass: 21408.248 Da / Num. of mol.: 1 / Mutation: S306A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HTRA2, OMI, PRSS25 / Production host: Escherichia coli (E. coli) / References: UniProt: O43464, HtrA2 peptidase |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.58 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.5 M Sodium acetate trihydrate pH 6.0, 2.0 M Sodium formate, 3% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Dec 31, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 4→40 Å / Num. obs: 11300 / % possible obs: 90.7 % / Redundancy: 4.24 % / Biso Wilson estimate: 76.05 Å2 / CC1/2: 0.967 / Net I/σ(I): 4.36 |
Reflection shell | Resolution: 4→4.1 Å / Redundancy: 3.78 % / Mean I/σ(I) obs: 1.27 / Num. unique obs: 1441 / CC1/2: 0.251 / % possible all: 90.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1LCY Resolution: 4→39.53 Å / Cor.coef. Fo:Fc: 0.866 / Cor.coef. Fo:Fc free: 0.754 / SU B: 189.057 / SU ML: 1.065 / Cross valid method: THROUGHOUT / ESU R Free: 1.183 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 108.477 Å2
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Refinement step | Cycle: 1 / Resolution: 4→39.53 Å
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