- PDB-7utt: Structure of Non-hydrolyzable ATP (ApCpp) binds to Cyclic GMP AMP... -
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基本情報
登録情報
データベース: PDB / ID: 7utt
タイトル
Structure of Non-hydrolyzable ATP (ApCpp) binds to Cyclic GMP AMP synthase (cGAS) through Mn coordination
要素
Cyclic GMP-AMP synthase
Palindromic DNA18
キーワード
Transferase/DNA / non-hydrolyzable ATP (ApCpp) / Transferase-DNA complex
機能・相同性
機能・相同性情報
regulation of type I interferon production / cyclic GMP-AMP synthase / 2',3'-cyclic GMP-AMP synthase activity / paracrine signaling / poly-ADP-D-ribose modification-dependent protein binding / negative regulation of DNA repair / regulation of immunoglobulin production / cGAS/STING signaling pathway / regulation of T cell activation / negative regulation of cGAS/STING signaling pathway ...regulation of type I interferon production / cyclic GMP-AMP synthase / 2',3'-cyclic GMP-AMP synthase activity / paracrine signaling / poly-ADP-D-ribose modification-dependent protein binding / negative regulation of DNA repair / regulation of immunoglobulin production / cGAS/STING signaling pathway / regulation of T cell activation / negative regulation of cGAS/STING signaling pathway / cGMP-mediated signaling / cellular response to exogenous dsRNA / regulation of immune response / positive regulation of type I interferon production / negative regulation of double-strand break repair via homologous recombination / nucleosome binding / positive regulation of defense response to virus by host / phosphatidylinositol-4,5-bisphosphate binding / activation of innate immune response / cAMP-mediated signaling / molecular condensate scaffold activity / determination of adult lifespan / positive regulation of cellular senescence / site of double-strand break / double-stranded DNA binding / defense response to virus / nuclear body / DNA repair / innate immune response / DNA damage response / chromatin binding / GTP binding / protein homodimerization activity / DNA binding / ATP binding / nucleus / metal ion binding / plasma membrane / cytosol 類似検索 - 分子機能
根拠: isothermal titration calorimetry, As a non-hydrolyzable ATP, ApCpp can interact with cGAS. Divalent metal Mn (or Mg) coordinates ApCpp tri-phosphate to negative charged catalytic residues. Zn ...根拠: isothermal titration calorimetry, As a non-hydrolyzable ATP, ApCpp can interact with cGAS. Divalent metal Mn (or Mg) coordinates ApCpp tri-phosphate to negative charged catalytic residues. Zn is involved in Zink finger formation at the cGAS dimer interface.
解像度: 2.04→29.67 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.956 / SU B: 7.214 / SU ML: 0.108 / 交差検証法: THROUGHOUT / ESU R: 0.17 / ESU R Free: 0.146 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : RESIDUAL ONLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2104
2000
2.9 %
RANDOM
Rwork
0.1811
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obs
0.1819
66963
99.23 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK