+Open data
-Basic information
Entry | Database: PDB / ID: 7ulw | ||||||
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Title | CryoEM structure of human LACTB filament | ||||||
Components | Serine beta-lactamase-like protein LACTB, mitochondrial | ||||||
Keywords | MEMBRANE PROTEIN / serine protease / filament / tumor suppressor / membrane / mitochondria / beta lactamase | ||||||
Function / homology | Function and homology information Hydrolases; Acting on peptide bonds (peptidases) / regulation of lipid metabolic process / lipid metabolic process / peptidase activity / mitochondrion / proteolysis / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.1 Å | ||||||
Authors | Bennett, J.A. / Steward, L.R. / Aydin, H. | ||||||
Funding support | United States, 1items
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Citation | Journal: PLoS Biol / Year: 2022 Title: The structure of the human LACTB filament reveals the mechanisms of assembly and membrane binding. Authors: Jeremy A Bennett / Lottie R Steward / Johannes Rudolph / Adam P Voss / Halil Aydin / Abstract: Mitochondria are complex organelles that play a central role in metabolism. Dynamic membrane-associated processes regulate mitochondrial morphology and bioenergetics in response to cellular demand. ...Mitochondria are complex organelles that play a central role in metabolism. Dynamic membrane-associated processes regulate mitochondrial morphology and bioenergetics in response to cellular demand. In tumor cells, metabolic reprogramming requires active mitochondrial metabolism for providing key metabolites and building blocks for tumor growth and rapid proliferation. To counter this, the mitochondrial serine beta-lactamase-like protein (LACTB) alters mitochondrial lipid metabolism and potently inhibits the proliferation of a variety of tumor cells. Mammalian LACTB is localized in the mitochondrial intermembrane space (IMS), where it assembles into filaments to regulate the efficiency of essential metabolic processes. However, the structural basis of LACTB polymerization and regulation remains incompletely understood. Here, we describe how human LACTB self-assembles into micron-scale filaments that increase their catalytic activity. The electron cryo-microscopy (cryoEM) structure defines the mechanism of assembly and reveals how highly ordered filament bundles stabilize the active state of the enzyme. We identify and characterize residues that are located at the filament-forming interface and further show that mutations that disrupt filamentation reduce enzyme activity. Furthermore, our results provide evidence that LACTB filaments can bind lipid membranes. These data reveal the detailed molecular organization and polymerization-based regulation of human LACTB and provide new insights into the mechanism of mitochondrial membrane organization that modulates lipid metabolism. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7ulw.cif.gz | 410.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ulw.ent.gz | 337.5 KB | Display | PDB format |
PDBx/mmJSON format | 7ulw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7ulw_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 7ulw_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 7ulw_validation.xml.gz | 72.1 KB | Display | |
Data in CIF | 7ulw_validation.cif.gz | 106.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ul/7ulw ftp://data.pdbj.org/pub/pdb/validation_reports/ul/7ulw | HTTPS FTP |
-Related structure data
Related structure data | 26595MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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-Components
#1: Protein | Mass: 51318.648 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: LACTB, MRPL56, UNQ843/PRO1781 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: P83111, Hydrolases; Acting on peptide bonds (peptidases) |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-Sample preparation
Component | Name: LACTB / Type: COMPLEX Details: Uniprot ID: P83111 - HUMAN LACTB, Serine beta-lactamase-like protein LACTB, mitochondrial Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Value: 0.51 MDa / Experimental value: NO |
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Escherichia coli BL21(DE3) (bacteria) |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 0.3 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Specimen support | Grid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R1.2/1.3 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1500 nm / Nominal defocus min: 500 nm |
Image recording | Average exposure time: 3.985 sec. / Electron dose: 59.58 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.19.2_4158: / Classification: refinement | ||||||||||||||||||||||||
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EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Helical symmerty | Angular rotation/subunit: -48.258 ° / Axial rise/subunit: 21.582 Å / Axial symmetry: C1 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 248548 / Symmetry type: HELICAL | ||||||||||||||||||||||||
Refine LS restraints |
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