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- PDB-7ult: Crystal Structure of SARS-CoV-2 Nsp16/10 Heterodimer Apo-Form. -

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Basic information

Entry
Database: PDB / ID: 7ult
TitleCrystal Structure of SARS-CoV-2 Nsp16/10 Heterodimer Apo-Form.
Components
  • 2'-O-methyltransferase
  • Non-structural protein 10
KeywordsTRANSFERASE / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID / SARS-CoV-2 / nsp16/10
Function / homology
Function and homology information


protein guanylyltransferase activity / RNA endonuclease activity producing 3'-phosphomonoesters, hydrolytic mechanism / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Maturation of replicase proteins / TRAF3-dependent IRF activation pathway / ISG15-specific peptidase activity ...protein guanylyltransferase activity / RNA endonuclease activity producing 3'-phosphomonoesters, hydrolytic mechanism / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Maturation of replicase proteins / TRAF3-dependent IRF activation pathway / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs / snRNP Assembly / Translation of Replicase and Assembly of the Replication Transcription Complex / Replication of the SARS-CoV-2 genome / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / double membrane vesicle viral factory outer membrane / host cell endoplasmic reticulum-Golgi intermediate compartment / SARS coronavirus main proteinase / 5'-3' DNA helicase activity / 3'-5'-RNA exonuclease activity / host cell endosome / symbiont-mediated degradation of host mRNA / mRNA guanylyltransferase / symbiont-mediated suppression of host ISG15-protein conjugation / G-quadruplex RNA binding / symbiont-mediated suppression of host toll-like receptor signaling pathway / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / omega peptidase activity / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / SARS-CoV-2 modulates host translation machinery / host cell Golgi apparatus / symbiont-mediated suppression of host NF-kappaB cascade / symbiont-mediated perturbation of host ubiquitin-like protein modification / DNA helicase / methyltransferase cap1 activity / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / single-stranded RNA binding / regulation of autophagy / viral protein processing / host cell perinuclear region of cytoplasm / lyase activity / host cell endoplasmic reticulum membrane / RNA helicase / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont-mediated suppression of host gene expression / copper ion binding / viral translational frameshifting / symbiont-mediated activation of host autophagy / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / RNA-directed RNA polymerase activity / DNA-templated transcription / lipid binding / host cell nucleus / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding / membrane
Similarity search - Function
RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / NSP12 RNA-dependent RNA polymerase, coronavirus / : ...RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / NSP12 RNA-dependent RNA polymerase, coronavirus / : / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Coronavirus Nsp12 Interface domain profile. / : / : / Coronavirus Nonstructural protein 13, 1B domain / Coronavirus Non-structural protein 13, zinc-binding domain / Coronavirus Nonstructural protein 13, stalk domain / Nidovirus 2-O-methyltransferase / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Non-structural protein NSP15, N-terminal domain superfamily, coronavirus / Non-structural protein NSP15, middle domain superfamily / Coronavirus replicase NSP15, N-terminal oligomerization / Nonstructural protein 15, middle domain, coronavirus / Nonstructural protein 13, 1B domain, coronavirus / Coronavirus replicase NSP15, middle domain / Coronavirus replicase NSP15, N-terminal oligomerisation / Nidovirus 3'-5' exoribonuclease domain / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Non-structural protein NSP16, coronavirus-like / Non-structural protein 14, coronavirus / RNA polymerase, N-terminal, coronavirus / Coronavirus 2'-O-methyltransferase / Coronavirus proofreading exoribonuclease / Coronavirus RNA-dependent RNA polymerase, N-terminal / Nonstructural protein 13, zinc-binding domain, coronavirus-like / Coronaviridae zinc-binding (CV ZBD) domain profile. / Arterivirus Nsp11 N-terminal/Coronavirus NSP15 middle domain / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. / Nidoviral uridylate-specific endoribonuclease (NendoU) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Endoribonuclease EndoU-like / NendoU domain, nidovirus / Coronavirus replicase NSP15, uridylate-specific endoribonuclease / : / Lipocalin signature. / DNA2/NAM7 helicase-like, C-terminal / AAA domain / (+) RNA virus helicase core domain / (+)RNA virus helicase core domain profile. / Non-structural protein NSP3, SUD-N (Mac2) domain, betacoronavirus / Sarbecovirus Nsp3c-N domain profile. / Non-structural protein NSP3, N-terminal, betacoronavirus / Polyprotein cleavage domain PL2pro superfamily, betacoronavirus / Non-structural protein NSP3, SUD-N (Mac2) domain superfamily, betacoronavirus / Betacoronavirus SUD-C domain / Betacoronavirus replicase NSP3, N-terminal / NSP1 globular domain superfamily, betacoronavirus / Non-structural protein 2, SARS-CoV-like / Betacoronavirus single-stranded poly(A) binding domain / Betacoronavirus Nsp3c-M domain profile. / NSP1, globular domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain superfamily, betacoronavirus / NSP1, C-terminal domain, betacoronavirus / Betacoronavirus replicase NSP1 / Betacoronavirus (BetaCoV) Nsp1 C-terminal domain profile. / : / Betacoronavirus Nsp3e group 2-specific marker (G2M) domain profile. / Betacoronavirus Nsp3c-C domain profile. / Betacoronavirus Nsp3e nucleic acid-binding (NAB) domain profile. / DPUP/SUD, C-terminal, betacoronavirus / Non-structural protein NSP3, nucleic acid-binding domain, betacoronavirus / Non-structural protein NSP3A domain-like superfamily / Non-structural protein NSP3, nucleic acid-binding domain superfamily, betacoronavirus / Non-structural protein 6, betacoronavirus / Betacoronavirus nucleic acid-binding (NAB) / Papain-like viral protease, palm and finger domains, coronavirus / Papain-like protease, N-terminal domain superfamily, coronavirus / Carbamoyl-phosphate synthase subdomain signature 2. / Coronavirus replicase NSP2, N-terminal / : / Coronavirus replicase NSP2, C-terminal / Coronavirus (CoV) Nsp2 middle domain profile. / NSP1, globular domain, alpha/betacoronavirus / Coronavirus (CoV) Nsp1 globular domain profile. / Coronavirus (CoV) Nsp2 N-terminal domain profile. / Coronavirus (CoV) Nsp2 C-terminal domain profile. / Nonstructural protein 2, N-terminal domain, coronavirus / Non-structural protein 2, C-terminal domain, coronavirus / NSP3, second ubiquitin-like (Ubl) domain, coronavirus / Coronavirus Nsp3a Ubl domain profile. / Coronavirus Nsp3d Ubl domain profile. / NSP3, first ubiquitin-like (Ubl) domain, coronavirus / Peptidase family C16 domain profile. / : / : / Coronavirus replicase NSP7 / Coronavirus 3Ecto domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp7 cofactor domain profile.
Similarity search - Domain/homology
FORMIC ACID / Replicase polyprotein 1ab
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å
AuthorsMinasov, G. / Shuvalova, L. / Brunzelle, J.S. / Rosas-Lemus, M. / Kiryukhina, O. / Satchell, K.J.F. / Center for Structural Genomics of Infectious Diseases (CSGID)
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Crystal Structure of SARS-CoV-2 Nsp16/10 Heterodimer Apo-Form.
Authors: Minasov, G. / Shuvalova, L. / Brunzelle, J.S. / Rosas-Lemus, M. / Kiryukhina, O. / Satchell, K.J.F. / Center for Structural Genomics of Infectious Diseases (CSGID)
History
DepositionApr 5, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 13, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 2'-O-methyltransferase
B: Non-structural protein 10
C: 2'-O-methyltransferase
D: Non-structural protein 10
hetero molecules


Theoretical massNumber of molelcules
Total (without water)98,34933
Polymers97,1214
Non-polymers1,22829
Water13,836768
1
A: 2'-O-methyltransferase
B: Non-structural protein 10
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,10515
Polymers48,5612
Non-polymers54513
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3490 Å2
ΔGint-46 kcal/mol
Surface area20390 Å2
MethodPISA
2
C: 2'-O-methyltransferase
D: Non-structural protein 10
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,24418
Polymers48,5612
Non-polymers68316
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4090 Å2
ΔGint-51 kcal/mol
Surface area19630 Å2
MethodPISA
Unit cell
Length a, b, c (Å)166.681, 166.681, 98.550
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number154
Space group name H-MP3221

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Components

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Protein , 2 types, 4 molecules ACBD

#1: Protein 2'-O-methyltransferase / NSP16


Mass: 33556.426 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Plasmid: pMCSG53 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): magic
References: UniProt: P0DTD1, Transferases; Transferring one-carbon groups; Methyltransferases
#2: Protein Non-structural protein 10 / NSP10


Mass: 15004.114 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Plasmid: pMCSG53 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P0DTD1

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Non-polymers , 4 types, 797 molecules

#3: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Na
#4: Chemical
ChemComp-FMT / FORMIC ACID


Mass: 46.025 Da / Num. of mol.: 17 / Source method: obtained synthetically / Formula: CH2O2
#5: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn / Feature type: SUBJECT OF INVESTIGATION
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 768 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.1 Å3/Da / Density % sol: 70 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: Protein: 5.3 mg/ml (nsp10/nsp16 1:1), 0.15M Sodium chloride, 0.01M Tris pH 7.5 , 5% Glycerol; Screen: ComPAS (F10), 0.4M Potassium/Sodium tartrate.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 1.12723 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Oct 7, 2021
RadiationMonochromator: DIAMOND / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.12723 Å / Relative weight: 1
ReflectionResolution: 1.9→30 Å / Num. obs: 123235 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 11.1 % / Biso Wilson estimate: 35.8 Å2 / CC1/2: 0.998 / CC star: 0.999 / Rmerge(I) obs: 0.13 / Rpim(I) all: 0.041 / Rrim(I) all: 0.137 / Rsym value: 0.13 / Χ2: 1.024 / Net I/σ(I): 19.1
Reflection shellResolution: 1.9→1.93 Å / Redundancy: 10 % / Rmerge(I) obs: 1.403 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 5985 / CC1/2: 0.577 / CC star: 0.855 / Rpim(I) all: 0.458 / Rsym value: 1.403 / Χ2: 1.005 / % possible all: 97.8

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Processing

Software
NameVersionClassification
REFMAC5.8.0267refinement
PDB_EXTRACT3.27data extraction
HKL-3000data reduction
HKL-3000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6w4h

6w4h


Resolution: 1.9→29.9 Å / Cor.coef. Fo:Fc: 0.967 / Cor.coef. Fo:Fc free: 0.956 / SU B: 4.593 / SU ML: 0.067 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.093 / ESU R Free: 0.091 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
RfactorNum. reflection% reflectionSelection details
Rfree0.1875 6205 5 %RANDOM
Rwork0.1644 ---
obs0.1656 117003 99.66 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso max: 135.34 Å2 / Biso mean: 35.067 Å2 / Biso min: 17.74 Å2
Baniso -1Baniso -2Baniso -3
1-0.31 Å20.15 Å20 Å2
2--0.31 Å2-0 Å2
3----1 Å2
Refinement stepCycle: final / Resolution: 1.9→29.9 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6390 0 63 784 7237
Biso mean--50.33 44.97 -
Num. residues----827
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0050.0136804
X-RAY DIFFRACTIONr_bond_other_d0.0010.0156311
X-RAY DIFFRACTIONr_angle_refined_deg1.3251.649242
X-RAY DIFFRACTIONr_angle_other_deg0.3421.58314565
X-RAY DIFFRACTIONr_dihedral_angle_1_deg3.1415872
X-RAY DIFFRACTIONr_dihedral_angle_2_deg28.424.013304
X-RAY DIFFRACTIONr_dihedral_angle_3_deg8.899151134
X-RAY DIFFRACTIONr_dihedral_angle_4_deg7.2251521
X-RAY DIFFRACTIONr_chiral_restr0.060.2893
X-RAY DIFFRACTIONr_gen_planes_refined0.0550.027912
X-RAY DIFFRACTIONr_gen_planes_other0.0480.021566
LS refinement shellResolution: 1.901→1.95 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.279 528 -
Rwork0.264 8329 -
all-8857 -
obs--98.33 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.4163-0.33391.22230.7605-0.21573.2025-0.1175-0.13350.35040.09240.007-0.0535-0.27780.1230.11050.1599-0.01230.00080.0247-0.01460.104393.15535.73049.7375
21.324-0.01210.51761.44220.26690.9595-0.02390.0282-0.1276-0.13180.07590.05750.1219-0.052-0.05210.1826-0.00780.00110.03680.01080.052584.964619.1836-4.2721
31.12710.21150.02971.32450.61342.0326-0.0479-0.0516-0.07140.00150.1161-0.12450.16020.2245-0.06820.13360.02360.00750.0758-0.00340.074396.665923.67484.0557
41.1404-0.23510.26621.64790.34914.1146-0.15560.00720.2704-0.18250.12410.1217-0.32590.11630.03150.1743-0.0149-0.04620.03090.03260.113982.646235.6609-6.4261
56.2471-0.28691.15434.0472-1.55074.6318-0.02570.3285-0.1128-0.43350.0186-0.76610.03240.61710.00710.2164-0.01040.150.2728-0.05970.1883108.597322.4032-14.9944
61.67643.3732-0.63197.6141-1.95440.8136-0.01760.0819-0.1548-0.13530.1103-0.13570.08630.0462-0.09270.24480.03640.05850.0896-0.03820.10293.94115.2736-10.4125
71.1747-0.9763-2.22998.7731-0.17224.84210.08640.0602-0.1833-0.4087-0.23990.53380.0188-0.1650.15340.1138-0.0882-0.12350.13340.02150.361655.29056.6642-12.3998
82.4032-0.1020.35822.6374-0.09031.485-0.0370.1774-0.0472-0.28150.0810.31020.0099-0.1106-0.0440.1954-0.0396-0.09020.06180.01590.077169.369422.8538-14.9891
94.07894.518-0.645511.3355-0.39870.93930.00550.3474-0.4072-0.6065-0.090.05820.19120.15850.08450.30610.0313-0.08040.1688-0.07450.207571.26487.4011-19.0305
101.56790.3490.05581.6846-0.68663.1467-0.00790.2205-0.1327-0.2940.06280.38490.1993-0.1836-0.05490.236-0.0499-0.14670.08110.00150.157663.239320.4315-19.4115
113.0627-2.08015.07595.9806-1.27179.58730.1885-0.0405-0.0395-0.6725-0.16290.40070.2653-0.1276-0.02560.3233-0.0167-0.21050.183-0.00170.140860.222425.0483-30.7886
1211.91581.05763.498624.377-8.02184.66460.5844-1.3144-0.6751-0.2952-0.5547-0.08640.6211-0.0398-0.02980.29710.1105-0.00690.28080.07530.091161.960337.3926-43.3927
133.9503-0.3098-0.43441.34950.68591.630.02090.24-0.37690.0033-0.0318-0.10030.14950.2770.01090.21060.04510.01060.1179-0.06080.134691.3215-37.9057-24.4052
141.26440.2723-0.07870.91450.20791.44480.02030.03520.103-0.0076-0.00630.0898-0.0915-0.0027-0.01390.15790.0140.0160.02790.00220.070181.8787-20.6062-9.8027
153.0822-3.2093-3.09333.58282.04179.28190.30320.4125-0.0243-0.2904-0.32650.0223-0.7-0.78270.02330.24220.02080.02570.23090.01890.011889.7573-18.6855-32.6911
161.0679-0.0606-0.17710.59370.33491.7688-0.01020.1259-0.0506-0.0710.0672-0.05690.01120.237-0.05710.15440.02170.0210.0833-0.01040.065192.7874-27.8524-17.5732
179.90753.33084.20732.41251.9383.3590.0073-0.0351-0.34450.16970.1663-0.1720.10550.2479-0.17350.12710.02810.01020.0723-0.01550.020892.4717-26.4573.5066
180.8694-1.3980.89395.4281-2.06783.1179-0.0525-0.03990.15550.1556-0.0204-0.3466-0.15560.39020.07290.0758-0.03750.00330.0955-0.02810.039997.6114-16.8024-1.223
190.57442.0023-0.06527.7523-1.25821.39670.0884-0.02480.07550.3976-0.08420.263-0.1598-0.0285-0.00420.09070.05860.00430.059-0.00460.233655.3744-0.93-7.3249
2010.72064.7329-2.74864.2679-0.07514.730.05060.21340.1914-0.2841-0.14890.34380.0994-0.08260.09830.06850.0441-0.04720.08790.03780.162351.4421-11.0705-15.8161
211.68830.23340.21323.029-0.11081.2030.0494-0.11340.16620.0999-0.02120.3781-0.0433-0.2234-0.02820.11570.01230.03880.0694-0.02240.147565.2614-21.9307-4.5716
223.9819-0.4846-0.25869.639-2.37813.2566-0.0441-0.31190.36870.5959-0.03580.3227-0.2626-0.05630.07990.19450.01560.03920.0484-0.09370.241867.6039-5.3815-0.6875
232.12550.4072-1.74072.9066-1.2593.470.0316-0.06930.13170.15170.11690.5497-0.2146-0.3279-0.14850.12490.04030.07490.091-0.03620.222159.5846-18.4879-1.335
248.192.4384-0.55765.1245-2.40829.49610.3858-0.40280.19690.4646-0.10680.6055-0.1606-0.3736-0.2790.18220.00840.16380.1614-0.04150.190253.7054-21.82028.4461
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A6799 - 6826
2X-RAY DIFFRACTION2A6827 - 6947
3X-RAY DIFFRACTION3A6948 - 7032
4X-RAY DIFFRACTION4A7033 - 7052
5X-RAY DIFFRACTION5A7053 - 7077
6X-RAY DIFFRACTION6A7078 - 7096
7X-RAY DIFFRACTION7B4271 - 4289
8X-RAY DIFFRACTION8B4290 - 4332
9X-RAY DIFFRACTION9B4333 - 4348
10X-RAY DIFFRACTION10B4349 - 4374
11X-RAY DIFFRACTION11B4375 - 4386
12X-RAY DIFFRACTION12B4387 - 4392
13X-RAY DIFFRACTION13C6799 - 6827
14X-RAY DIFFRACTION14C6828 - 6930
15X-RAY DIFFRACTION15C6931 - 6945
16X-RAY DIFFRACTION16C6946 - 7046
17X-RAY DIFFRACTION17C7047 - 7063
18X-RAY DIFFRACTION18C7064 - 7096
19X-RAY DIFFRACTION19D4271 - 4282
20X-RAY DIFFRACTION20D4283 - 4289
21X-RAY DIFFRACTION21D4290 - 4332
22X-RAY DIFFRACTION22D4333 - 4346
23X-RAY DIFFRACTION23D4347 - 4374
24X-RAY DIFFRACTION24D4375 - 4385

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