+Open data
-Basic information
Entry | Database: PDB / ID: 7ucr | ||||||
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Title | Joint X-ray/neutron structure of the Sarcin-Ricin loop RNA | ||||||
Components | Sarcin-Ricin loop RNA | ||||||
Keywords | RNA / Sarcin-Ricin loop | ||||||
Function / homology | DEUTERATED WATER / RNA / RNA (> 10) Function and homology information | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / NEUTRON DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1 Å | ||||||
Authors | Harp, J.M. / Egli, M.E. / Pallan, P.S. / Coates, L. | ||||||
Funding support | 1items
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Citation | Journal: Nucleic Acids Res. / Year: 2022 Title: Cryo neutron crystallography demonstrates influence of RNA 2'-OH orientation on conformation, sugar pucker and water structure. Authors: Harp, J.M. / Lybrand, T.P. / Pallan, P.S. / Coates, L. / Sullivan, B. / Egli, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7ucr.cif.gz | 62.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ucr.ent.gz | 42.7 KB | Display | PDB format |
PDBx/mmJSON format | 7ucr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7ucr_validation.pdf.gz | 368.6 KB | Display | wwPDB validaton report |
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Full document | 7ucr_full_validation.pdf.gz | 371.3 KB | Display | |
Data in XML | 7ucr_validation.xml.gz | 2.1 KB | Display | |
Data in CIF | 7ucr_validation.cif.gz | 3.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uc/7ucr ftp://data.pdbj.org/pub/pdb/validation_reports/uc/7ucr | HTTPS FTP |
-Related structure data
Related structure data | 3dvzS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: RNA chain | Mass: 8744.255 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-DOD / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment |
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-Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.37 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.233 mM RNA, 1.0 M ammonium sulfate, 2 mM magnesium chloride, 2 mM manganese chloride, 16.5 mM -3-(N-morpholino)propanesulfonic acid. |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Biso Wilson estimate: 8.3 Å2 / Entry-ID: 7UCR
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Reflection shell |
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-Processing
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Refinement | SU ML: 0.1008 / Cross valid method: FREE R-VALUE / Method to determine structure: MOLECULAR REPLACEMENT / Phase error: 23.1382 / Shrinkage radii: 0.5 Å / VDW probe radii: 0.9 Å / Starting model: 3DVZ / Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 / Solvent model: FLAT BULK SOLVENT MODEL
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Refinement step | Cycle: LAST / Resolution: 1→16.05 Å
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Refine LS restraints |
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LS refinement shell |
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