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- PDB-7u8k: Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled o... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7u8k | ||||||||||||
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Title | Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments | ||||||||||||
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![]() | CONTRACTILE PROTEIN / CELL CYCLE / actin filament binding / actin filament severing / cytoskeletal assembly | ||||||||||||
Function / homology | ![]() actin filament fragmentation / positive regulation of actin filament depolymerization / actin filament severing / actin filament depolymerization / I band / cytoskeletal motor activator activity / sarcomere organization / muscle cell cellular homeostasis / tropomyosin binding / mesenchyme migration ...actin filament fragmentation / positive regulation of actin filament depolymerization / actin filament severing / actin filament depolymerization / I band / cytoskeletal motor activator activity / sarcomere organization / muscle cell cellular homeostasis / tropomyosin binding / mesenchyme migration / troponin I binding / myosin heavy chain binding / filamentous actin / actin filament bundle / skeletal muscle thin filament assembly / striated muscle thin filament / actin filament bundle assembly / skeletal muscle myofibril / actin monomer binding / skeletal muscle fiber development / stress fiber / skeletal muscle tissue development / titin binding / actin filament polymerization / filopodium / actin filament / cell motility / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / Z disc / nuclear matrix / calcium-dependent protein binding / actin filament binding / actin cytoskeleton / lamellipodium / cell body / hydrolase activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / magnesium ion binding / extracellular space / extracellular exosome / ATP binding / identical protein binding / cytoplasm Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||
Method | SOLID-STATE NMR / molecular dynamics | ||||||||||||
![]() | Kraus, J. / Russell, R. / Kudryashova, E. / Xu, C. / Katyal, N. / Kudryashov, D. / Perilla, J.R. / Polenova, T. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Magic angle spinning NMR structure of human cofilin-2 assembled on actin filaments reveals isoform-specific conformation and binding mode. Authors: Kraus, J. / Russell, R.W. / Kudryashova, E. / Xu, C. / Katyal, N. / Perilla, J.R. / Kudryashov, D.S. / Polenova, T. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 6.1 MB | Display | ![]() |
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PDB format | ![]() | 5.1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7m0gC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 41886.660 Da / Num. of mol.: 10 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P68135, Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement #2: Protein | Mass: 18789.672 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLID-STATE NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 50 mM / Label: cofilin condition / pH: 6.6 / Pressure: 1 atm / Temperature: 273 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 850 MHz |
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Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 9 | ||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 4 / Conformers submitted total number: 4 |