Entry Database : PDB / ID : 7u2p Structure visualization Downloads & linksTitle Structure of TcdA GTD in complex with RhoA ComponentsGlucosyltransferase TcdA Transforming protein RhoA DetailsKeywords TOXIN / GTDFunction / homology Function and homology informationFunction Domain/homology Component
RHO GTPases Activate ROCKs / SLIT2:ROBO1 increases RHOA activity / Sema4D mediated inhibition of cell attachment and migration / Sema4D induced cell migration and growth-cone collapse / RHO GTPases Activate Rhotekin and Rhophilins / RHO GTPases activate PKNs / Axonal growth stimulation / PCP/CE pathway / RHO GTPases activate KTN1 / Axonal growth inhibition (RHOA activation) ... RHO GTPases Activate ROCKs / SLIT2:ROBO1 increases RHOA activity / Sema4D mediated inhibition of cell attachment and migration / Sema4D induced cell migration and growth-cone collapse / RHO GTPases Activate Rhotekin and Rhophilins / RHO GTPases activate PKNs / Axonal growth stimulation / PCP/CE pathway / RHO GTPases activate KTN1 / Axonal growth inhibition (RHOA activation) / PI3K/AKT activation / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / EPHA-mediated growth cone collapse / ERBB2 Regulates Cell Motility / RHOC GTPase cycle / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / G beta:gamma signalling through PI3Kgamma / negative regulation of synapse assembly / GPVI-mediated activation cascade / EPHB-mediated forward signaling / RHO GTPases activate CIT / RHOA GTPase cycle / Ovarian tumor domain proteases / G alpha (12/13) signalling events / regulation of dendrite development / RHO GTPases Activate Formins / aortic valve formation / alpha-beta T cell lineage commitment / mitotic cleavage furrow formation / bone trabecula morphogenesis / regulation of modification of synaptic structure / endothelial tube lumen extension / skeletal muscle satellite cell migration / positive regulation of vascular associated smooth muscle contraction / negative regulation of vascular associated smooth muscle cell migration / angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure / Roundabout signaling pathway / negative regulation of intracellular steroid hormone receptor signaling pathway / regulation of neural precursor cell proliferation / cleavage furrow formation / regulation of modification of postsynaptic actin cytoskeleton / positive regulation of smooth muscle contraction / regulation of osteoblast proliferation / forebrain radial glial cell differentiation / cell junction assembly / apical junction assembly / regulation of systemic arterial blood pressure by endothelin / cellular response to chemokine / negative regulation of cell migration involved in sprouting angiogenesis / negative regulation of cell size / beta selection / establishment of epithelial cell apical/basal polarity / regulation of modification of postsynaptic structure / VEGFA-VEGFR2 Pathway / negative regulation of oxidative phosphorylation / negative regulation of motor neuron apoptotic process / stress-activated protein kinase signaling cascade / apolipoprotein A-I-mediated signaling pathway / GTP metabolic process / positive regulation of podosome assembly / negative regulation of cell-substrate adhesion / positive regulation of alpha-beta T cell differentiation / ossification involved in bone maturation / odontogenesis / motor neuron apoptotic process / positive regulation of leukocyte adhesion to vascular endothelial cell / wound healing, spreading of cells / microtubule depolymerization / host cell cytosol / apical junction complex / Transferases; Glycosyltransferases; Hexosyltransferases / regulation of neuron projection development / negative chemotaxis / Rho GDP-dissociation inhibitor binding / myosin binding / positive regulation of actin filament polymerization / stress fiber assembly / glycosyltransferase activity / positive regulation of cytokinesis / androgen receptor signaling pathway / cerebral cortex cell migration / cellular response to cytokine stimulus / negative regulation of vascular associated smooth muscle cell proliferation / cleavage furrow / regulation of calcium ion transport / semaphorin-plexin signaling pathway / Rho protein signal transduction / negative regulation of neuron differentiation / mitotic spindle assembly / endothelial cell migration / positive regulation of T cell migration / regulation of microtubule cytoskeleton organization / negative regulation of reactive oxygen species biosynthetic process / negative regulation of canonical NF-kappaB signal transduction / cytoplasmic microtubule organization / skeletal muscle tissue development / cysteine-type peptidase activity / regulation of cell migration / positive regulation of stress fiber assembly / cytoskeleton organization Similarity search - Function TcdA/TcdB toxin, N-terminal helical domain / TcdB toxin N-terminal helical domain / TcdA/TcdB toxin, catalytic glycosyltransferase domain / TcdA/TcdB catalytic glycosyltransferase domain / TcdA/TcdB toxin, pore forming domain / TcdA/TcdB pore forming domain / CGT/MARTX, cysteine protease (CPD) domain / CGT/MARTX, cysteine protease (CPD) domain superfamily / Peptidase C80 family / CGT/MARTX cysteine protease (CPD) domain profile. ... TcdA/TcdB toxin, N-terminal helical domain / TcdB toxin N-terminal helical domain / TcdA/TcdB toxin, catalytic glycosyltransferase domain / TcdA/TcdB catalytic glycosyltransferase domain / TcdA/TcdB toxin, pore forming domain / TcdA/TcdB pore forming domain / CGT/MARTX, cysteine protease (CPD) domain / CGT/MARTX, cysteine protease (CPD) domain superfamily / Peptidase C80 family / CGT/MARTX cysteine protease (CPD) domain profile. / Choline-binding repeat / Putative cell wall binding repeat / Cell wall/choline-binding repeat / Cell wall-binding repeat profile. / Small GTPase Rho / small GTPase Rho family profile. / Nucleotide-diphospho-sugar transferases / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homologyBiological species Clostridioides difficile (bacteria)Homo sapiens (human)Method X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution : 2.596 Å DetailsAuthors Baohua, C. / Zheng, L. / Kay, P. / Rongsheng, J. Funding support United States, 5items Details Hide detailsOrganization Grant number Country National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI125704 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI139087 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI158503 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R21AI156092 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) P30 GM124165 United States
CitationJournal : Sci Rep / Year : 2022Title : Structure of the glucosyltransferase domain of TcdA in complex with RhoA provides insights into substrate recognition.Authors : Chen, B. / Liu, Z. / Perry, K. / Jin, R. History Deposition Feb 24, 2022 Deposition site : RCSB / Processing site : RCSBRevision 1.0 May 4, 2022 Provider : repository / Type : Initial releaseRevision 1.1 May 11, 2022 Group : Structure summary / Category : struct / Item : _struct.titleRevision 1.2 Jun 8, 2022 Group : Database references / Category : citation / citation_authorItem : _citation.country / _citation.journal_abbrev ... _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.name Revision 1.3 Oct 18, 2023 Group : Data collection / Derived calculations / Refinement descriptionCategory : atom_type / chem_comp_atom ... atom_type / chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model Item : _atom_type.pdbx_N_electrons / _atom_type.pdbx_scat_Z
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