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- PDB-7tds: Labrum-interacting protein from saliva LIPS-2 (34K-2) from Aedes ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7tds | ||||||||||||||||||
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Title | Labrum-interacting protein from saliva LIPS-2 (34K-2) from Aedes albopictus, native data | ||||||||||||||||||
![]() | 34k2 salivary protein | ||||||||||||||||||
![]() | SIGNALING PROTEIN / mosquito / saliva / feeding behaviour / protein-protein interaction / proboscis / cuticle | ||||||||||||||||||
Function / homology | NITRATE ION / Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2![]() | ||||||||||||||||||
Biological species | ![]() ![]() | ||||||||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||||||||
![]() | Gabrieli, P. / Forneris, F. | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum. Authors: Arnoldi, I. / Mancini, G. / Fumagalli, M. / Gastaldi, D. / D'Andrea, L. / Bandi, C. / Di Venere, M. / Iadarola, P. / Forneris, F. / Gabrieli, P. | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.5 KB | Display | ![]() |
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PDB format | ![]() | 105.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.2 KB | Display | ![]() |
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Full document | ![]() | 450.1 KB | Display | |
Data in XML | ![]() | 13.3 KB | Display | |
Data in CIF | ![]() | 17.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7tdrC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34899.723 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: The construct was deprived of the signal sequence (uniprot residues 1-20) and cloning introduced an additional N-terminal GLY and three C-terminal ALA residues. Source: (gene. exp.) ![]() ![]() Tissue: Saliva / Plasmid: pET-SUMO / Production host: ![]() ![]() | ||||||||
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#2: Chemical | ChemComp-SO4 / #3: Chemical | #4: Chemical | ChemComp-NA / | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 6.13 Å3/Da / Density % sol: 79.94 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: 1 M (NH4)2SO4, 1 M LI2SO4, 0.1 M KNO3 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Feb 2, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.966 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→47.26 Å / Num. obs: 43932 / % possible obs: 98.5 % / Redundancy: 7.9 % / Biso Wilson estimate: 49.41 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.072 / Rpim(I) all: 0.035 / Net I/σ(I): 14.2 |
Reflection shell | Resolution: 2.2→2.27 Å / Redundancy: 8 % / Rmerge(I) obs: 1.426 / Mean I/σ(I) obs: 1.3 / Num. unique obs: 3689 / CC1/2: 0.616 / Rpim(I) all: 0.728 / % possible all: 97.8 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 64.95 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→46.43 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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