+
Open data
-
Basic information
Entry | Database: PDB / ID: 7tdq | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of KSHV KicGAS/ORF52 | ||||||||||||
![]() | Tegument protein ORF52 | ||||||||||||
![]() | VIRAL PROTEIN / tegument protein / oligomerization / DNA-binding | ||||||||||||
Function / homology | Herpesvirus BLRF2 / Herpesvirus BLRF2 protein / : / viral tegument / double-stranded DNA binding / host cell cytoplasm / Tegument protein ORF52![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Yin, Q. / Tian, Y. / Bhowmik, D. / Zhu, F. | ||||||||||||
Funding support | ![]()
| ||||||||||||
![]() | ![]() Title: Structural basis of higher order oligomerization of KSHV inhibitor of cGAS. Authors: Bhowmik, D. / Tian, Y. / Wang, B. / Zhu, F. / Yin, Q. | ||||||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 93.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 60.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.3 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 425.7 KB | Display | |
Data in XML | ![]() | 8.6 KB | Display | |
Data in CIF | ![]() | 11 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2oa5S S: Starting model for refinement |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
| ||||||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 9623.736 Da / Num. of mol.: 2 / Mutation: L62M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.81 Å3/Da / Density % sol: 56.28 % |
---|---|
Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1 microL of protein (10 mg/ml) + 1 microL of reservoir solution of 9% (v/v) PEG3350, 100mM Na-citrate pH 4.5 + 0.2microL of the detergent additive n-Octyl-N,N-dimethyl-3-ammonio-1-propanesulfonate |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jul 31, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 15886 / % possible obs: 98.3 % / Redundancy: 4.9 % / Biso Wilson estimate: 52.29 Å2 / CC1/2: 0.991 / CC star: 0.008 / Rmerge(I) obs: 0.128 / Rpim(I) all: 0.062 / Rrim(I) all: 0.142 / Rsym value: 0.102 / Χ2: 1.911 / Net I/σ(I): 19.78 |
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.595 / Mean I/σ(I) obs: 1.32 / Num. unique obs: 1481 / CC1/2: 0.751 / CC star: 0.926 / Rpim(I) all: 0.351 / Rrim(I) all: 0.696 / Rsym value: 0.497 / Χ2: 0.661 / % possible all: 90.4 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 2OA5 Resolution: 2.5→49.77 Å / SU ML: 0.3788 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 31.4291 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 64.27 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→49.77 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: 11.6371299098 Å / Origin y: 45.0483533591 Å / Origin z: 56.2487140621 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group | Selection details: all |