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Open data
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Basic information
Entry | Database: PDB / ID: 7t19 | ||||||
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Title | Rev1 Ternary Complex with dGTP and Ca2+ | ||||||
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![]() | TRANSFERASE/DNA / ![]() ![]() | ||||||
Function / homology | ![]() deoxycytidyl transferase activity / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / Termination of translesion DNA synthesis / error-free translesion synthesis / error-prone translesion synthesis / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() | ||||||
![]() | Freudenthal, B.D. / Weaver, T.M. | ||||||
Funding support | 1items
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![]() | ![]() Title: Mechanism of nucleotide discrimination by the translesion synthesis polymerase Rev1. Authors: Weaver, T.M. / Click, T.H. / Khoang, T.H. / Todd Washington, M. / Agarwal, P.K. / Freudenthal, B.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 139.6 KB | Display | ![]() |
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PDB format | ![]() | 93.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7t18C ![]() 7t1aC ![]() 7t1bC ![]() 6x6zS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-DNA chain , 2 types, 2 molecules PT
#1: DNA chain | Mass: 3814.474 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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#2: DNA chain | Mass: 5052.290 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Protein , 1 types, 1 molecules A
#3: Protein | ![]() Mass: 51285.938 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: REV1, YOR346W, O6339 / Production host: ![]() ![]() ![]() References: UniProt: P12689, ![]() |
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-Non-polymers , 3 types, 215 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/DGT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/DGT.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | #5: Chemical | ChemComp-DGT / | ![]() #6: Water | ChemComp-HOH / | ![]() |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.79 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 15-23% PEG3350, 200 mM ammonium nitrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: DECTRIS PILATUS 200K / Detector: PIXEL / Date: Jan 13, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.01→25 Å / Num. obs: 36239 / % possible obs: 99.5 % / Redundancy: 4.4 % / Biso Wilson estimate: 30.54 Å2 / CC1/2: 0.993 / Net I/σ(I): 6.87 |
Reflection shell | Resolution: 2.03→2.06 Å / Num. unique obs: 1772 / CC1/2: 0.443 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB entry 6X6Z Resolution: 2.01→24.59 Å / SU ML: 0.2543 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.7351 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.52 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.01→24.59 Å
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Refine LS restraints |
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LS refinement shell |
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