+
Open data
-
Basic information
Entry | Database: PDB / ID: 7sxd | ||||||
---|---|---|---|---|---|---|---|
Title | NMR solution structure TnC-TnI chimera | ||||||
![]() | Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle chimera | ||||||
![]() | METAL BINDING PROTEIN / cardiac troponin / calcium sensitizer / troponin activator | ||||||
Function / homology | ![]() regulation of systemic arterial blood pressure by ischemic conditions / troponin C binding / regulation of muscle filament sliding speed / troponin T binding / diaphragm contraction / regulation of ATP-dependent activity / cardiac Troponin complex / cardiac myofibril / troponin complex / regulation of smooth muscle contraction ...regulation of systemic arterial blood pressure by ischemic conditions / troponin C binding / regulation of muscle filament sliding speed / troponin T binding / diaphragm contraction / regulation of ATP-dependent activity / cardiac Troponin complex / cardiac myofibril / troponin complex / regulation of smooth muscle contraction / regulation of muscle contraction / transition between fast and slow fiber / negative regulation of ATP-dependent activity / Striated Muscle Contraction / regulation of cardiac muscle contraction by calcium ion signaling / response to metal ion / ventricular cardiac muscle tissue morphogenesis / heart contraction / troponin I binding / skeletal muscle contraction / vasculogenesis / calcium channel inhibitor activity / cardiac muscle contraction / Ion homeostasis / sarcomere / intracellular calcium ion homeostasis / calcium-dependent protein binding / actin filament binding / heart development / actin binding / protein domain specific binding / calcium ion binding / protein kinase binding / protein homodimerization activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Poppe, L. / Hartman, J.J. / Romero, A. / Reagan, J.D. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Structural and Thermodynamic Model for the Activation of Cardiac Troponin. Authors: Poppe, L. / Hartman, J.J. / Romero, A. / Reagan, J.D. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 445.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 378.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 7supC ![]() 7svcC ![]() 7swgC ![]() 7swiC ![]() 7sxcC C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
Other databases |
|
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: Protein | Mass: 14222.121 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-
Sample preparation
Details | Type: solution Contents: 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O Label: sample_1 / Solvent system: 95% H2O/5% D2O |
---|---|
Sample | Conc.: 1 mM / Component: TnC-TnI chimera / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 50 mM / Label: sample_1 / pH: 7.2 / Pressure: 1 atm / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III HD / Manufacturer: Bruker / Model: AVANCE III HD / Field strength: 800 MHz |
---|
-
Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 4 | ||||||||||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 10 |