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- PDB-7sx7: Crystal structure of broadly neutralizing antibody N49P9.3-FR3-3 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7sx7 | ||||||||||||
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Title | Crystal structure of broadly neutralizing antibody N49P9.3-FR3-3 Fab in complex with HIV-1 Clade A/E strain 93TH057 gp120 core | ||||||||||||
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![]() | IMMUNE SYSTEM/VIRAL PROTEIN / HIV-1 / VRC01-CLASS ANTIBODY / CD4 BINDING SITE / CLADE A/E 93TH057 GP120 / VIRAL PROTEIN-IMMUNE SYSTEM COMPLEX / N49P9.3-FR3-3 / IMMUNE SYSTEM / IMMUNE SYSTEM-VIRAL PROTEIN complex | ||||||||||||
Function / homology | Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / viral envelope / clade A/E 93TH057 HIV-1 gp120 core![]() | ||||||||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Tolbert, W.D. / Pazgier, M. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of broadly neutralizing antibody N49P9.3-FR3-3 Fab in complex with HIV-1 Clade A/E strain 93TH057 gp120 core Authors: Tolbert, W.D. / Pazgier, M. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 626 KB | Display | ![]() |
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PDB format | ![]() | 515.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 512.4 KB | Display | ![]() |
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Full document | ![]() | 544.5 KB | Display | |
Data in XML | ![]() | 61.5 KB | Display | |
Data in CIF | ![]() | 85.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6oz3S S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-Antibody , 2 types, 4 molecules HBLC
#2: Antibody | Mass: 25050.166 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #3: Antibody | Mass: 21763.316 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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-Protein / Sugars , 2 types, 25 molecules GA![](data/chem/img/NAG.gif)
![](data/chem/img/NAG.gif)
#1: Protein | Mass: 39356.613 Da / Num. of mol.: 2 / Mutation: H375S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Sugar | ChemComp-NAG / |
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-Non-polymers , 2 types, 446 molecules ![](data/chem/img/EPE.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.59 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 10% PEG 3350 0.1 M HEPES pH 7.0 0.1 M magnesium chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 19, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97946 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→50 Å / Num. obs: 73767 / % possible obs: 82.7 % / Redundancy: 1.8 % / CC1/2: 0.949 / Rmerge(I) obs: 0.135 / Rpim(I) all: 0.135 / Net I/σ(I): 6.9 |
Reflection shell | Resolution: 2.15→2.19 Å / Redundancy: 1.4 % / Rmerge(I) obs: 0.645 / Mean I/σ(I) obs: 1 / Num. unique obs: 2786 / CC1/2: 0.394 / Rpim(I) all: 0.645 / % possible all: 63.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6OZ3 Resolution: 2.15→46.66 Å / SU ML: 0.37 / Cross valid method: FREE R-VALUE / σ(F): 1.94 / Phase error: 30.88 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.15→46.66 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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