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- PDB-7sqw: Structure of the KcsA-W67F mutant with the activation gate in the... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7sqw | |||||||||
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Title | Structure of the KcsA-W67F mutant with the activation gate in the closed conformation | |||||||||
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![]() | MEMBRANE PROTEIN / channel / Fab complex / C-type inactivation | |||||||||
Function / homology | Chem-1EM / NONAN-1-OL / : ![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Cuello, L.G. / Labro, A.J. | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: The nonconducting W434F mutant adopts upon membrane depolarization an inactivated-like state that differs from wild-type Shaker-IR potassium channels. Authors: Coonen, L. / Martinez-Morales, E. / Van De Sande, D.V. / Snyders, D.J. / Cortes, D.M. / Cuello, L.G. / Labro, A.J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 218.1 KB | Display | ![]() |
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PDB format | ![]() | 174.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 666.9 KB | Display | ![]() |
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Full document | ![]() | 676.4 KB | Display | |
Data in XML | ![]() | 21.4 KB | Display | |
Data in CIF | ![]() | 28.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1k4cS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 10939.700 Da / Num. of mol.: 1 / Mutation: W67F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Non-polymers , 3 types, 7 molecules ![](data/chem/img/F09.gif)
![](data/chem/img/1EM.gif)
![](data/chem/img/K.gif)
![](data/chem/img/1EM.gif)
![](data/chem/img/K.gif)
#4: Chemical | ChemComp-F09 / |
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#5: Chemical | ChemComp-1EM / ( |
#6: Chemical | ChemComp-K / |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.89 Å3/Da / Density % sol: 68.36 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop Details: PEG400 , 50 mM magnesium acetate, 50 mM sodium acetate, 300 mM KCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 19, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2 Å / Relative weight: 1 |
Reflection | Resolution: 3.21→48.69 Å / Num. obs: 14014 / % possible obs: 99.8 % / Redundancy: 3.1 % / Biso Wilson estimate: 81.33 Å2 / CC1/2: 0.75 / Net I/σ(I): 14.7 |
Reflection shell | Resolution: 3.21→48.69 Å / Num. unique obs: 14012 / CC1/2: 0.5 / % possible all: 99.8 |
-Phasing
Phasing | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 1K4C Resolution: 3.21→48.69 Å / SU ML: 0.46 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 30.67 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 165.2 Å2 / Biso mean: 89.551 Å2 / Biso min: 34.18 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.21→48.69 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Origin x: -27.6765 Å / Origin y: -19.5068 Å / Origin z: -27.4419 Å
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Refinement TLS group |
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