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Open data
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Basic information
Entry | Database: PDB / ID: 7sog | ||||||
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Title | Thermostable actophorin | ||||||
![]() | Actophorin | ||||||
![]() | MOTOR PROTEIN / MUTAGENESIS | ||||||
Function / homology | ![]() actin filament fragmentation / actin filament severing / actin filament binding / actin cytoskeleton / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lieberman, R.L. / Quirk, S. | ||||||
Funding support | 1items
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![]() | ![]() Title: Structure and activity of a thermally stable mutant of Acanthamoeba actophorin. Authors: Quirk, S. / Lieberman, R.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 142.4 KB | Display | ![]() |
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PDB format | ![]() | 91.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 440.7 KB | Display | ![]() |
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Full document | ![]() | 441.8 KB | Display | |
Data in XML | ![]() | 14 KB | Display | |
Data in CIF | ![]() | 19.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7rtxS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15183.150 Da / Num. of mol.: 2 Mutation: S2P, H21K, Q22K, T27I, M30L, A32D, S33D, H41K, K53L, S54A, R59N, Q71E, T81V, T99A, T101S, S104A, V110D, A122D, S130K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-NI / #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / Details: PEG 8000, NISO4, MES pH6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 28, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→36.58 Å / Num. obs: 24315 / % possible obs: 94.92 % / Redundancy: 3.4 % / Biso Wilson estimate: 22.85 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.05504 / Rrim(I) all: 0.0655 / Net I/σ(I): 13.1 |
Reflection shell | Resolution: 1.74→1.802 Å / Rmerge(I) obs: 0.6171 / Mean I/σ(I) obs: 2.49 / Num. unique obs: 2410 / CC1/2: 0.769 / Rrim(I) all: 0.7272 / % possible all: 89.89 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7RTX Resolution: 1.74→36.58 Å / SU ML: 0.2074 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 23.696 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.12 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.74→36.58 Å
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Refine LS restraints |
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LS refinement shell |
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