PDB-7s5o: Crystal structure of Cytochrome c' beta from Nitrosomonas europae...

ID or keywords:

Entry

Database: PDB / ID: 7s5o

Title

Crystal structure of Cytochrome c' beta from Nitrosomonas europaea ATCC 19718

Components

Cytochrome_P460 domain-containing protein

Keywords

METAL BINDING PROTEIN / SSGCID / Cytochrome_P460 domain-containing protein / Nitrosomonas europaea / NE0824 / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease

Function / homology

Cytochrome P460 / Cytochrome P460 superfamily / Cytochrome P460 / ACETATE ION / HEME C / Cytochrome_P460 domain-containing protein

Function and homology information

Biological species

Nitrosomonas europaea (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

SAD / Resolution: 1.8 Å

Authors

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Funding support

United States, 1items

Citation

Journal: Biochemistry / Year: 2022
Title: Structural Characterization of Cytochrome c ' beta-Met from an Ammonia-Oxidizing Bacterium.
Authors: Abendroth, J. / Buchko, G.W. / Liew, F.N. / Nguyen, J.N. / Kim, H.J.

History

Deposition	Sep 11, 2021	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Mar 30, 2022	Provider: repository / Type: Initial release
Revision 1.1	Apr 13, 2022	Group: Structure summary / Category: struct / Item: _struct.title
Revision 1.2	Apr 20, 2022	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7s5o.cif.gz	215.1 KB	Display	PDBx/mmCIF format
PDB format	pdb7s5o.ent.gz	179 KB	Display	PDB format
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Validation report

Summary document	7s5o_validation.pdf.gz	501 KB	Display	wwPDB validaton report
Full document	7s5o_full_validation.pdf.gz	502.7 KB	Display
Data in XML	7s5o_validation.xml.gz	1.8 KB	Display
Data in CIF	7s5o_validation.cif.gz	10.3 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/s5/7s5o ftp://data.pdbj.org/pub/pdb/validation_reports/s5/7s5o	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - HomologyF&H Search
Other databases	TargetTrack: NieuA.20425.a

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#36 - Dec 2002 Cytochrome c similarity (1) #111 - Mar 2009 Hydrogenase similarity (1) #137 - May 2011 Cytochrome bc1 similarity (1) #22 - Oct 2001 Photosystem I similarity (1) #232 - Apr 2019 Proteins and Biominerals similarity (1) #59 - Nov 2004 Photosystem II similarity (1) #89 - May 2007 Aconitase and Iron Regulatory Protein 1 similarity (1)

Deposited unit

A: Cytochrome_P460 domain-containing protein

B: Cytochrome_P460 domain-containing protein

C: Cytochrome_P460 domain-containing protein

hetero molecules

59.8 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Cytochrome_P460 domain-containing protein

hetero molecules

defined by author
Evidence: gel filtration
19.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Cytochrome_P460 domain-containing protein

hetero molecules

defined by author
19.8 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

C: Cytochrome_P460 domain-containing protein

hetero molecules

defined by author
20 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	41.790, 88.170, 75.850
Angle α, β, γ (deg.)	90.000, 101.620, 90.000
Int Tables number	4
Space group name H-M	P12₁1

#1: Protein	Cytochrome_P460 domain-containing protein / NieuA.20425.a.K12 Mass: 19054.297 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) (bacteria) Strain: ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298 / Gene: NE0824 / Plasmid: NieuA.20425.a.K12 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q82W71
#2: Chemical	ChemComp-HEC / HEME C Mass: 618.503 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₃₄H₃₄FeN₄O₄ / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical	ChemComp-SO4 / SULFATE ION Mass: 96.063 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: SO₄
#4: Chemical	ChemComp-ACT / ACETATE ION Mass: 59.044 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₂H₃O₂
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 597 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.4 Å³/Da / Density % sol: 48.9 % / Mosaicity: 0.123 °
Crystal grow	Temperature: 287 K / Method: vapor diffusion, sitting drop / pH: 4.77 Details: Calibre Anatrace Top96 screen, condition #30 optimization screen: 100mM sodium acetate HCl pH 4.77, 2245mM ammonium sulfate) + 100mM sodium malonate pH 7.0; NieuA.20425.a.K12.PB00109 at ...Details: Calibre Anatrace Top96 screen, condition #30 optimization screen: 100mM sodium acetate HCl pH 4.77, 2245mM ammonium sulfate) + 100mM sodium malonate pH 7.0; NieuA.20425.a.K12.PB00109 at 10mg/ml, tray: 319288 F8; cryo: 7ul reservoir + 14ul 4M ammonium sulfate, 60sec soak; puck hyq3-1, For phasing: Anatrace Top96 screen, optimization screen around condition #30: 100mM Sodium acetate / HCl pH 5.0, 2245mM ammonium sulfate NieuA.20425.a.K12.PB00109 at 10mg/ml; tray: 318611 h8; puck rwi1-1. 3x 360deg were collected at the in-house system at Cu-Kalpha, 1.5418A

Crystal

Density Matthews: 2.4 Å³/Da / Density % sol: 48.9 % / Mosaicity: 0.123 °

Crystal grow

Temperature: 287 K / Method: vapor diffusion, sitting drop / pH: 4.77
Details: Calibre Anatrace Top96 screen, condition #30 optimization screen: 100mM sodium acetate HCl pH 4.77, 2245mM ammonium sulfate) + 100mM sodium malonate pH 7.0; NieuA.20425.a.K12.PB00109 at ...

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Data collection

Diffraction

ID	Mean temperature (K)	Crystal-ID	Serial crystal experiment
1	100	1	N
2	100	1	N

Diffraction source

Source	Site	Beamline	Type	ID	Wavelength (Å)
SYNCHROTRON	APS	21-ID-F		1	0.97872
ROTATING ANODE			RIGAKU FR-E+ SUPERBRIGHT	2	1.5418

Detector

Type	ID	Detector	Date	Details
RAYONIX MX-300	1	CCD	Mar 11, 2021	Beryllium Lenses
RIGAKU SATURN 944+	2	CCD	Jan 6, 2021

Radiation

ID	Monochromator	Protocol	Monochromatic (M) / Laue (L)	Scattering type	Wavelength-ID
1	Diamond [111]	SINGLE WAVELENGTH	M	x-ray	1
2	RIGAKU VARIMAX	SINGLE WAVELENGTH	M	x-ray	2

Radiation wavelength

ID	Wavelength (Å)	Relative weight
1	0.97872	1
2	1.5418	1

Reflection

Resolution: 1.8→50 Å / Num. obs: 48663 / % possible obs: 97.5 % / Observed criterion σ(I): -3 / Redundancy: 4.8 % / CC_1/2: 0.998 / R_merge(I) obs: 0.074 / R_rim(I) all: 0.083 / Χ²: 0.955 / Net I/σ(I): 14.69

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. unique obs	CC_1/2	R_rim(I) all	% possible all
1.8-1.85	3.1	0.355	2.91	2968	0.865	0.429	80.4
1.85-1.9	3.35	0.327	3.53	3216	0.893	0.389	89.7
1.9-1.95	3.91	0.276	4.8	3323	0.941	0.319	96.2
1.95-2.01	4.79	0.242	6.3	3407	0.965	0.272	99.8
2.01-2.08	5.27	0.213	7.8	3288	0.974	0.237	100
2.08-2.15	5.28	0.176	9.24	3139	0.982	0.195	100
2.15-2.23	5.27	0.149	10.73	3087	0.986	0.166	99.9
2.23-2.32	5.24	0.126	12.16	2985	0.988	0.14	99.9
2.32-2.43	5.29	0.114	13.37	2797	0.991	0.126	100
2.43-2.55	5.26	0.102	14.86	2695	0.993	0.113	99.8
2.55-2.68	5.26	0.09	16.55	2591	0.994	0.1	100
2.68-2.85	5.24	0.077	18.84	2433	0.995	0.086	99.8
2.85-3.04	5.22	0.068	21.48	2319	0.996	0.076	100
3.04-3.29	5.17	0.056	24.76	2125	0.997	0.062	99.9
3.29-3.6	5.14	0.047	28.33	1972	0.998	0.052	100
3.6-4.02	5.1	0.041	31.58	1786	0.998	0.046	99.9
4.02-4.65	5.12	0.037	33.93	1586	0.998	0.041	99.9
4.65-5.69	5.15	0.038	33.65	1321	0.998	0.042	99.9
5.69-8.05	5.01	0.04	32.57	1050	0.998	0.044	100
8.05-50	4.72	0.031	35.04	575	0.999	0.034	98.1

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Processing

Software

Name	Version	Classification
XDS		data reduction
XSCALE		data scaling
PHENIX	1.19	refinement
PDB_EXTRACT	3.27	data extraction
CRANK2		phasing
Coot		model building
SHELXD		phasing
PARROT		phasing
BUCCANEER		model building

Refinement

Method to determine structure:

SAD / Resolution: 1.8→35.94 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 18.39 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.1901	2095	4.31 %	0
R_work	0.1546	46557	-	-
obs	0.1561	48652	97.52 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 81.94 Å² / B_iso mean: 22.8387 Å² / B_iso min: 6.56 Å²

Refinement step

Cycle: final / Resolution: 1.8→35.94 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	3632	0	171	612	4415
B_iso mean	-	-	19.31	30.98	-
Num. residues	-	-	-	-	472

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 15

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
1.8-1.84	0.2455	102	0.2085	2524	2626	80
1.84-1.89	0.2465	150	0.1947	2790	2940	89
1.89-1.94	0.2611	127	0.1833	3011	3138	95
1.94-2	0.2002	152	0.1667	3168	3320	100
2-2.06	0.1985	149	0.1549	3137	3286	100
2.06-2.13	0.2032	148	0.1491	3165	3313	100
2.13-2.22	0.1695	140	0.1515	3198	3338	100
2.22-2.32	0.1901	129	0.1531	3220	3349	100
2.32-2.44	0.2157	153	0.153	3136	3289	100
2.44-2.6	0.1912	136	0.162	3188	3324	100
2.6-2.8	0.2011	156	0.1593	3181	3337	100
2.8-3.08	0.1832	138	0.1608	3170	3308	100
3.08-3.52	0.1721	139	0.1519	3199	3338	100
3.52-4.44	0.1492	137	0.1235	3227	3364	100
4.44-35.94	0.1979	139	0.1597	3243	3382	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	7.2881	1.209	-0.1906	3.6904	-0.0398	3.5859	-0.0222	0.9184	-0.3568	-0.4175	0.0796	-0.1518	0.1997	0.1363	-0.0101	0.2411	0.0196	0.0205	0.2305	-0.0039	0.0995	4.519	0.827	48.602
2	1.9657	-0.5404	-0.3359	1.7539	-0.3351	1.5194	-0.0057	0.0959	-0.1256	-0.0451	-0.0243	0.2161	0.0972	-0.2555	0.0526	0.0921	-0.0118	-0.0206	0.1505	-0.0359	0.1182	-9.345	4.111	58.783
3	4.9776	-3.8286	-0.6573	6.5782	-5.0847	9.4518	-0.0048	-0.093	-0.4207	-0.1037	0.2481	-0.1395	0.2811	-0.2504	-0.2275	0.1623	-0.0376	0.0231	0.0967	-0.0019	0.2461	-3.14	-11.055	64.376
4	1.9244	-2.4621	0.3061	5.7984	-2.136	2.8253	-0.1462	-0.0306	-0.0128	0.3688	-0.0467	0.0616	0.0424	-0.1683	0.1944	0.1181	-0.0126	-0.0151	0.0874	-0.0398	0.1192	-2.435	-0.974	59.987
5	6.2072	-1.1535	-0.1939	4.7307	1.1234	7.296	0.1147	0.4128	0.6479	-0.9082	-0.0297	0.6231	-1.2595	-0.7223	-0.0623	0.3519	0.1237	-0.07	0.3108	0.0099	0.2886	-16.51	22.303	55.261
6	7.4701	-0.8163	-0.4735	4.2839	0.8594	0.3297	0.0516	-0.1509	-0.3232	-0.0527	-0.1215	0.4277	-0.2623	-0.7107	0.0878	0.1363	0.0571	-0.044	0.2909	-0.0537	0.247	-19.115	13.94	59.864
7	2.7869	-0.002	-0.224	2.2452	0.0791	2.5231	0.0341	0.1286	-0.0573	-0.2592	-0.1667	-0.3567	0.2457	0.2376	0.1032	0.122	0.0185	0.0307	0.1007	-0.0164	0.1241	5.725	-0.499	54.614
8	4.1107	-1.0986	1.2785	4.0862	-2.0058	6.7346	-0.0979	-0.0081	-0.1014	0.1723	-0.0685	-0.4058	-0.1447	0.5708	0.1494	0.1156	0.0116	0.0078	0.121	-0.006	0.1379	8.802	2.076	57.933
9	4.1503	1.4015	1.5915	4.0989	1.6046	5.4779	-0.1345	0.4721	0.3611	-0.3439	-0.1089	0.0289	-0.7491	0.1364	0.2086	0.2038	0.0068	-0.0067	0.1615	0.0336	0.1277	2.111	10.863	48.776
10	3.7746	0.4035	-0.8463	4.018	-1.114	5.3989	-0.0332	-0.1922	-0.0873	0.1972	-0.0504	-0.2717	-0.1223	0.2665	0.0601	0.1156	-0.0043	-0.0154	0.0876	-0.0188	0.1069	4.594	4.406	67.371
11	8.2823	4.387	2.0575	5.7817	0.484	7.5318	0.0789	-0.3296	-0.502	0.1247	0.0237	0.4562	0.6056	-0.7549	-0.1007	0.1534	-0.0477	0.023	0.1975	0.0162	0.2557	-10.09	-5.523	68.275
12	2.5266	1.6726	0.4634	4.3978	1.1383	4.1769	0.0964	-0.2465	0.549	0.6717	-0.1324	0.2565	-0.3859	-0.3047	0.1059	0.2272	0.0166	0.0369	0.1335	-0.0471	0.1794	-3.149	25.038	86.131
13	3.4913	-0.5379	-1.0689	2.6066	-1.3447	1.2169	-0.0468	-0.3732	0.1479	0.3841	0.0619	-0.2355	-0.2122	0.4531	-0.0179	0.1679	-0.0203	-0.0346	0.1396	-0.0136	0.1403	12.436	19.919	82.587
14	2.0179	0.0235	-0.561	1.9994	-1.4743	2.1997	0.079	0.1456	0.0581	-0.0615	0.0153	-0.0683	-0.0629	-0.0297	-0.0255	0.1123	-0.0077	0.0128	0.0716	-0.0155	0.1144	8.887	19.746	73.246
15	2.2547	-0.4882	-1.798	8.0356	-2.0095	5.7435	0.0622	-0.0004	0.0409	0.5982	-0.2358	-0.4565	-0.0447	0.159	0.1503	0.1435	-0.0093	-0.0329	0.1856	0.0054	0.1072	8.647	8.419	90.383
16	1.5073	0.3897	-0.753	4.0553	-3.5446	4.1762	-0.0244	-0.122	-0.0186	-0.0356	0.0318	-0.0425	0.2069	0.0771	-0.1127	0.1498	-0.0042	0.0061	0.0917	-0.0166	0.0922	5.056	15.501	82.394
17	3.3345	-0.4377	-0.0031	4.9291	-2.7874	9.3204	0.0369	0.9162	0.7033	-0.2971	-0.102	-0.0473	-0.3457	0.3257	-0.0106	0.1901	-0.0034	0.046	0.2314	0.0869	0.2312	13.208	29.51	59.575
18	2.3115	0.0028	-0.8095	2.346	0.0413	2.2407	0.1564	0.0376	0.203	-0.0153	-0.0398	-0.1854	-0.2054	0.1383	-0.0711	0.1098	0.0008	-0.0115	0.088	-0.0239	0.0909	10.596	23.314	73.303
19	1.6239	-0.2151	-0.2289	2.2866	0.1074	1.6792	-0.1632	-1.0753	-0.4105	0.6736	0.2941	0.719	0.2396	-0.7024	0.044	0.2207	0.0302	0.1219	0.3878	0.0799	0.2724	-10.516	15.234	89.784
20	3.6486	0.4112	-0.4363	4.4842	-0.3837	2.5015	-0.0129	-0.2305	-0.1374	0.2595	-0.0324	0.5402	-0.0054	-0.2369	0.0346	0.1082	0.0164	0.0251	0.1499	-0.0132	0.1414	-6.439	16.602	81.604
21	2.5892	0.3317	-0.0404	3.5422	0.8988	1.9037	-0.4207	0.0389	0.6409	-0.8261	-0.1415	0.6525	-0.8924	-0.2971	-0.046	0.2171	0.0577	-0.0718	0.1509	-0.0342	0.2636	-2.799	29.267	76.638
22	3.4033	-0.9687	-0.2423	3.7487	0.293	2.517	-0.0454	-0.0281	-0.2309	-0.1778	-0.0599	0.3232	0.1061	-0.2027	0.0583	0.1213	-0.0141	-0.0158	0.1203	-0.0202	0.1023	-1.621	9.031	75.697
23	8.3428	1.7408	4.0518	8.1744	-1.6179	2.8619	0.0212	-0.4138	-0.1866	0.385	0.0112	-0.768	0.3525	0.2235	-0.073	0.1681	0.0448	-0.0187	0.188	-0.0295	0.1995	14.422	7.835	82.533
24	4.1211	-0.4696	-0.3653	2.6435	1.0506	2.9712	0.0321	0.0167	0.0372	0.0208	-0.0555	-0.0107	-0.0911	-0.1431	0.0201	0.1492	-0.0141	0.0066	0.1213	-0.0019	0.0531	3.767	0.015	100.718
25	6.0739	-1.7145	1.6917	3.4945	1.494	3.2603	-0.0466	-1.0228	0.2404	0.7111	-0.0084	-0.0348	0.2028	0.008	0.0406	0.2277	-0.0432	-0.0083	0.2747	-0.0294	0.1311	5.28	5.322	116.357
26	2.0836	-1.9675	-2.0937	4.4486	3.2946	4.6566	-0.0255	0.1291	-0.144	-0.0362	0.0755	-0.2111	0.2061	0.1245	-0.0087	0.1381	-0.0107	-0.0025	0.1715	-0.0225	0.1495	11.593	-9.472	91.587
27	5.0974	-0.7439	-3.3088	1.0738	0.2319	2.6093	0.1399	-0.0253	0.004	0.0615	-0.2071	0.2129	-0.2137	-0.4197	0.0299	0.1424	-0.0031	0.0236	0.1917	-0.0416	0.1355	-5.625	1.203	106.085
28	3.3333	0.7707	-0.496	2.7824	-0.8214	1.9136	0.0671	-0.2157	-0.2041	0.0192	-0.0106	0.219	0.523	-0.4804	-0.0614	0.1969	-0.0594	0.0225	0.1664	-0.0361	0.1656	-3.8	-5.703	105.711
29	6.9965	1.8343	1.0857	2.8071	0.9803	4.8312	-0.1236	0.0545	0.6425	0.132	-0.1128	-0.1983	-0.4316	0.3722	0.1548	0.1805	-0.0176	-0.0595	0.2248	0.0186	0.1751	14.445	3.316	112.34

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 22:33 )	A	22 - 33
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 34:58 )	A	34 - 58
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 59:67 )	A	59 - 67
4	X-RAY DIFFRACTION	4	( CHAIN A AND RESID 68:83 )	A	68 - 83
5	X-RAY DIFFRACTION	5	( CHAIN A AND RESID 84:93 )	A	84 - 93
6	X-RAY DIFFRACTION	6	( CHAIN A AND RESID 94:103 )	A	94 - 103
7	X-RAY DIFFRACTION	7	( CHAIN A AND RESID 104:122 )	A	104 - 122
8	X-RAY DIFFRACTION	8	( CHAIN A AND RESID 123:135 )	A	123 - 135
9	X-RAY DIFFRACTION	9	( CHAIN A AND RESID 136:147 )	A	136 - 147
10	X-RAY DIFFRACTION	10	( CHAIN A AND RESID 148:163 )	A	148 - 163
11	X-RAY DIFFRACTION	11	( CHAIN A AND RESID 164:176 )	A	164 - 176
12	X-RAY DIFFRACTION	12	( CHAIN B AND RESID 22:33 )	B	22 - 33
13	X-RAY DIFFRACTION	13	( CHAIN B AND RESID 34:45 )	B	34 - 45
14	X-RAY DIFFRACTION	14	( CHAIN B AND RESID 46:58 )	B	46 - 58
15	X-RAY DIFFRACTION	15	( CHAIN B AND RESID 59:67 )	B	59 - 67
16	X-RAY DIFFRACTION	16	( CHAIN B AND RESID 68:83 )	B	68 - 83
17	X-RAY DIFFRACTION	17	( CHAIN B AND RESID 84:93 )	B	84 - 93
18	X-RAY DIFFRACTION	18	( CHAIN B AND RESID 94:113 )	B	94 - 113
19	X-RAY DIFFRACTION	19	( CHAIN B AND RESID 114:122 )	B	114 - 122
20	X-RAY DIFFRACTION	20	( CHAIN B AND RESID 123:135 )	B	123 - 135
21	X-RAY DIFFRACTION	21	( CHAIN B AND RESID 136:147 )	B	136 - 147
22	X-RAY DIFFRACTION	22	( CHAIN B AND RESID 148:163 )	B	148 - 163
23	X-RAY DIFFRACTION	23	( CHAIN B AND RESID 164:176 )	B	164 - 176
24	X-RAY DIFFRACTION	24	( CHAIN C AND RESID 0:58 )	C	0 - 58
25	X-RAY DIFFRACTION	25	( CHAIN C AND RESID 59:75 )	C	59 - 75
26	X-RAY DIFFRACTION	26	( CHAIN C AND RESID 76:103 )	C	76 - 103
27	X-RAY DIFFRACTION	27	( CHAIN C AND RESID 104:122 )	C	104 - 122
28	X-RAY DIFFRACTION	28	( CHAIN C AND RESID 123:163 )	C	123 - 163
29	X-RAY DIFFRACTION	29	( CHAIN C AND RESID 164:180 )	C	164 - 180

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