+Open data
-Basic information
Entry | Database: PDB / ID: 7s55 | ||||||
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Title | NMR Solution Structure of Cter 27 | ||||||
Components | Cliotide T10 | ||||||
Keywords | ANTIMICROBIAL PROTEIN / cyclotide peptide bracelet / cyclic cysteine knot | ||||||
Function / homology | Cyclotide, bracelet, conserved site / Cyclotides bracelet subfamily signature. / Cyclotides profile. / Cyclotide / Cyclotide superfamily / Cyclotide family / defense response / Cliotide T10 Function and homology information | ||||||
Biological species | Clitoria ternatea (plant) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Harvey, P.J. / Dang, T.T. / Craik, D.J. | ||||||
Funding support | 1items
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Citation | Journal: Peptide Science / Year: 2022 Title: Mutagenesis of cyclotide Cter 27 exemplifies a robust folding strategy for bracelet cyclotides Authors: Dang, T.T. / Harvey, P.J. / Chan, L.Y. / Huang, Y.-H. / Kaas, Q. / Craik, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7s55.cif.gz | 171.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7s55.ent.gz | 144.2 KB | Display | PDB format |
PDBx/mmJSON format | 7s55.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s5/7s55 ftp://data.pdbj.org/pub/pdb/validation_reports/s5/7s55 | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3271.913 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Clitoria ternatea (plant) / References: UniProt: C0HKG2 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: nd Not defined / Label: conditions_1 / pH: 3.0 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III HD / Manufacturer: Bruker / Model: AVANCE III HD / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |