+Open data
-Basic information
Entry | Database: PDB / ID: 7raz | |||||||||
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Title | Small conductance mechanosensitive channel MscS | |||||||||
Components | Small-conductance mechanosensitive channel | |||||||||
Keywords | TRANSPORT PROTEIN / small conductance mechanosensitive channel MscS / NCMN / ion channel / membrane protein | |||||||||
Function / homology | Function and homology information intracellular water homeostasis / mechanosensitive monoatomic ion channel activity / monoatomic ion transmembrane transport / protein homooligomerization / membrane / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Escherichia coli 'BL21-GoldpLysS AG' | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / negative staining / cryo EM / Resolution: 3.4 Å | |||||||||
Authors | Catalano, C. / Ben-Hail, D. / Qiu, W. / des Georges, A. / Guo, Y. | |||||||||
Funding support | United States, 2items
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Citation | Journal: To Be Published Title: Small conductance mechanosensitive channel MscS Authors: Catalano, C. / Ben-Hail, D. / Qiu, W. / des Georges, A. / Guo, Y. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7raz.cif.gz | 236.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7raz.ent.gz | 193.1 KB | Display | PDB format |
PDBx/mmJSON format | 7raz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ra/7raz ftp://data.pdbj.org/pub/pdb/validation_reports/ra/7raz | HTTPS FTP |
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-Related structure data
Related structure data | 24390MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 30922.898 Da / Num. of mol.: 7 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) Strain: BL21-Gold(DE3)pLysS AG / Gene: mscS, yggB, b2924, JW2891 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) Strain (production host): BL21-Gold(DE3)pLysS AG / References: UniProt: P0C0S1 #2: Chemical | ChemComp-PTY / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Small conductance mechanosensitive channel MscS / Type: CELL / Entity ID: #1 / Source: NATURAL |
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Source (natural) | Organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) |
Source (recombinant) | Organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) |
Buffer solution | pH: 7.8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: YES / Vitrification applied: YES |
EM staining | Type: NEGATIVE / Material: 2% uranyl acetate |
Specimen support | Grid type: UltrAuFoil R0.6/1 |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE-PROPANE / Humidity: 100 % / Chamber temperature: 277.15 K |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy |
Image recording | Average exposure time: 10 sec. / Electron dose: 61.05 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 1352 |
Image scans | Movie frames/image: 40 |
-Processing
Software | Name: PHENIX / Version: 1.19_4092: / Classification: refinement | ||||||||||||||||||||||||||||
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EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||
Particle selection | Num. of particles selected: 500000 | ||||||||||||||||||||||||||||
Symmetry | Point symmetry: C7 (7 fold cyclic) | ||||||||||||||||||||||||||||
3D reconstruction | Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 275000 / Symmetry type: POINT | ||||||||||||||||||||||||||||
Atomic model building | Protocol: OTHER / Space: REAL | ||||||||||||||||||||||||||||
Atomic model building | PDB-ID: 2OAU | ||||||||||||||||||||||||||||
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